Analysis of antibody diversity: V‐D‐J mRNA nucleotide sequence of four anti‐GAT monoclonal antibodies. A paucigene system using alternate D‐J recombinations to generate functionally similar hypervariable regions
The nucleotide sequence of four anti‐(Glu60‐Ala30‐Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2‐terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These s...
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| Veröffentlicht in: | The EMBO journal 1983, Vol.2 (6), p.867-872 |
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| creator | Rocca‐Serra, J. Matthes, H.W. Kaartinen, M. Milstein, C. Thèze, J. Fougereau, M. |
| description | The nucleotide sequence of four anti‐(Glu60‐Ala30‐Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2‐terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These sequences, which are highly conserved, indicate that anti‐GAT antibodies expressing the same public idiotypic specificities represent a paucigene system, which uses at least two D‐J combinations leading to functionally similar hypervariable regions involved in the recognition of the dominant Glu‐Tyr determinant. D regions are encoded by D genes which are closely related either to the D‐SP2 or the D.FL16 germ line gene cores. |
| doi_str_mv | 10.1002/j.1460-2075.1983.tb01515.x |
| format | Article |
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A paucigene system using alternate D‐J recombinations to generate functionally similar hypervariable regions</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Rocca‐Serra, J. ; Matthes, H.W. ; Kaartinen, M. ; Milstein, C. ; Thèze, J. ; Fougereau, M.</creator><creatorcontrib>Rocca‐Serra, J. ; Matthes, H.W. ; Kaartinen, M. ; Milstein, C. ; Thèze, J. ; Fougereau, M.</creatorcontrib><description>The nucleotide sequence of four anti‐(Glu60‐Ala30‐Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2‐terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These sequences, which are highly conserved, indicate that anti‐GAT antibodies expressing the same public idiotypic specificities represent a paucigene system, which uses at least two D‐J combinations leading to functionally similar hypervariable regions involved in the recognition of the dominant Glu‐Tyr determinant. D regions are encoded by D genes which are closely related either to the D‐SP2 or the D.FL16 germ line gene cores.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1983.tb01515.x</identifier><identifier>PMID: 6416834</identifier><language>eng</language><publisher>England</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - genetics ; Antibody Diversity ; Base Sequence ; Genes ; Immunoglobulin Heavy Chains - genetics ; Immunoglobulin Idiotypes - genetics ; Immunoglobulin Variable Region - genetics ; Mice ; Mice, Inbred BALB C ; Recombination, Genetic ; RNA, Messenger - genetics</subject><ispartof>The EMBO journal, 1983, Vol.2 (6), p.867-872</ispartof><rights>1983 European Molecular Biology Organization</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4245-37e81b79b9a86d274b571e4a37646faae6edbcb279d034f70b16fb3711b557be3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC555202/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC555202/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,4026,27930,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6416834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rocca‐Serra, J.</creatorcontrib><creatorcontrib>Matthes, H.W.</creatorcontrib><creatorcontrib>Kaartinen, M.</creatorcontrib><creatorcontrib>Milstein, C.</creatorcontrib><creatorcontrib>Thèze, J.</creatorcontrib><creatorcontrib>Fougereau, M.</creatorcontrib><title>Analysis of antibody diversity: V‐D‐J mRNA nucleotide sequence of four anti‐GAT monoclonal antibodies. A paucigene system using alternate D‐J recombinations to generate functionally similar hypervariable regions</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>The nucleotide sequence of four anti‐(Glu60‐Ala30‐Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2‐terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These sequences, which are highly conserved, indicate that anti‐GAT antibodies expressing the same public idiotypic specificities represent a paucigene system, which uses at least two D‐J combinations leading to functionally similar hypervariable regions involved in the recognition of the dominant Glu‐Tyr determinant. D regions are encoded by D genes which are closely related either to the D‐SP2 or the D.FL16 germ line gene cores.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - genetics</subject><subject>Antibody Diversity</subject><subject>Base Sequence</subject><subject>Genes</subject><subject>Immunoglobulin Heavy Chains - genetics</subject><subject>Immunoglobulin Idiotypes - genetics</subject><subject>Immunoglobulin Variable Region - genetics</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Recombination, Genetic</subject><subject>RNA, Messenger - genetics</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkk9u1DAYxSMEKkPhCEgWC3YT7CSOnUosQimFqoCEClvLdr5MPXLsqZ0MzY4jcD92nISkM4xghVhYtvze7_mPXpI8IzglGGcv1ikpSrzMMKMpqXie9goTSmh6ey9ZHKT7yQJnJVkWhFcPk0cxrjHGlDNylByVBSl5XiySH7WTdowmIt8i6XqjfDOixmwhRNOPJ-jLz2_fX0_jAnWfPtTIDdqC700DKMLNAE7DTLZ-CHf45Dyvr1DnndfWT9m_Qw3EFNVoIwdtVuAmfIw9dGiIxq2QtD0EJ3tAu7MCaN8pM-0Y7yLqPZqZMBvawel5V1o7omg6Y2VA1-MGwlYGI5WFiV7N2OPkQStthCf7-Tj5_Obs6vTt8vLj-bvT-nKpi6ygy5wBJ4pVqpK8bDJWKMoIFDJnZVG2UkIJjdIqY1WD86JlWJGyVTkjRFHKFOTHyctd7mZQHTQaXB-kFZtgOhlG4aURfyvOXIuV3wpKaYaziX--54OfvjT2ojNRg7XSgR-i4JjllBP-TyPJOa8ILSbjyc6og48xQHu4DMFirpBYi7knYu6JmCsk9hUStxP89M_nHNB9Zya93ulfjYXxP5LF2ftXF3fr_BfDzOJG</recordid><startdate>1983</startdate><enddate>1983</enddate><creator>Rocca‐Serra, J.</creator><creator>Matthes, H.W.</creator><creator>Kaartinen, M.</creator><creator>Milstein, C.</creator><creator>Thèze, J.</creator><creator>Fougereau, M.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1983</creationdate><title>Analysis of antibody diversity: V‐D‐J mRNA nucleotide sequence of four anti‐GAT monoclonal antibodies. A paucigene system using alternate D‐J recombinations to generate functionally similar hypervariable regions</title><author>Rocca‐Serra, J. ; Matthes, H.W. ; Kaartinen, M. ; Milstein, C. ; Thèze, J. ; Fougereau, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4245-37e81b79b9a86d274b571e4a37646faae6edbcb279d034f70b16fb3711b557be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - genetics</topic><topic>Antibody Diversity</topic><topic>Base Sequence</topic><topic>Genes</topic><topic>Immunoglobulin Heavy Chains - genetics</topic><topic>Immunoglobulin Idiotypes - genetics</topic><topic>Immunoglobulin Variable Region - genetics</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Recombination, Genetic</topic><topic>RNA, Messenger - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rocca‐Serra, J.</creatorcontrib><creatorcontrib>Matthes, H.W.</creatorcontrib><creatorcontrib>Kaartinen, M.</creatorcontrib><creatorcontrib>Milstein, C.</creatorcontrib><creatorcontrib>Thèze, J.</creatorcontrib><creatorcontrib>Fougereau, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rocca‐Serra, J.</au><au>Matthes, H.W.</au><au>Kaartinen, M.</au><au>Milstein, C.</au><au>Thèze, J.</au><au>Fougereau, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of antibody diversity: V‐D‐J mRNA nucleotide sequence of four anti‐GAT monoclonal antibodies. A paucigene system using alternate D‐J recombinations to generate functionally similar hypervariable regions</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1983</date><risdate>1983</risdate><volume>2</volume><issue>6</issue><spage>867</spage><epage>872</epage><pages>867-872</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><abstract>The nucleotide sequence of four anti‐(Glu60‐Ala30‐Tyr10)n (GAT) monoclonal gamma 1 heavy chain mRNAs was determined from codon 10 to 120. This sequence overlaps with the NH2‐terminal amino acid sequence, allowing elucidation of the complete protein sequence encompassing regions VH, D and JH. These sequences, which are highly conserved, indicate that anti‐GAT antibodies expressing the same public idiotypic specificities represent a paucigene system, which uses at least two D‐J combinations leading to functionally similar hypervariable regions involved in the recognition of the dominant Glu‐Tyr determinant. D regions are encoded by D genes which are closely related either to the D‐SP2 or the D.FL16 germ line gene cores.</abstract><cop>England</cop><pmid>6416834</pmid><doi>10.1002/j.1460-2075.1983.tb01515.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0261-4189 |
| ispartof | The EMBO journal, 1983, Vol.2 (6), p.867-872 |
| issn | 0261-4189 1460-2075 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Animals Antibodies, Monoclonal - genetics Antibody Diversity Base Sequence Genes Immunoglobulin Heavy Chains - genetics Immunoglobulin Idiotypes - genetics Immunoglobulin Variable Region - genetics Mice Mice, Inbred BALB C Recombination, Genetic RNA, Messenger - genetics |
| title | Analysis of antibody diversity: V‐D‐J mRNA nucleotide sequence of four anti‐GAT monoclonal antibodies. A paucigene system using alternate D‐J recombinations to generate functionally similar hypervariable regions |
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