Orthogonal Mass Spectrometry-Based Footprinting for Epitope Mapping and Structural Characterization: The IL‑6 Receptor upon Binding of Protein Therapeutics

Higher-order structure (HOS) is a crucial determinant for the biological functions and quality attributes of protein therapeutics. Mass spectrometry (MS)-based protein footprinting approaches play an important role in elucidating the relationship between protein biophysical properties and structure....

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Veröffentlicht in:	Analytical chemistry (Washington) 2017-07, Vol.89 (14), p.7742-7749
Hauptverfasser:	Li, Ke Sherry, Chen, Guodong, Mo, Jingjie, Huang, Richard Y.-C, Deyanova, Ekaterina G, Beno, Brett R, O’Neil, Steve R, Tymiak, Adrienne A, Gross, Michael L
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Binding sites Biophysics Carboxyl group Chemistry Deuterium Deuterium Exchange Measurement Epitope Mapping epitopes Footprinting Humans Hydrogen-deuterium exchange Interleukin 6 Interleukin 6 receptors Interleukins ligands Mapping Mass Spectrometry Mass spectroscopy moieties Oxidation Peptide mapping photochemistry Protein Binding Protein Conformation Protein Footprinting Protein structure Proteins Quality management Receptors, Interleukin-6 - chemistry Selectivity Spectroscopy Structural analysis therapeutics
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