The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis
Mycoplasma pulmonis is a wall-less eubacterium belonging to the Mollicutes (trivial name, mycoplasmas) and responsible for murine respiratory diseases. The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported amo...
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| Veröffentlicht in: | Nucleic acids research 2001-05, Vol.29 (10), p.2145-2153 |
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| creator | Chambaud, I Heilig, R Ferris, S Barbe, V Samson, D Galisson, F Moszer, I Dybvig, K Wróblewski, H Viari, A Rocha, E P Blanchard, A |
| description | Mycoplasma pulmonis is a wall-less eubacterium belonging to the Mollicutes (trivial name, mycoplasmas) and responsible for murine respiratory diseases. The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glyco-protease are predicted virulence factors. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now. |
| doi_str_mv | 10.1093/nar/29.10.2145 |
| format | Article |
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The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glyco-protease are predicted virulence factors. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now.</description><identifier>ISSN: 1362-4962</identifier><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/29.10.2145</identifier><identifier>PMID: 11353084</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>England: Oxford Publishing Limited (England)</publisher><subject>Animals ; Antigens, Bacterial - genetics ; Antigens, Bacterial - immunology ; Base Composition ; Biochemistry ; Biochemistry, Molecular Biology ; Codon, Terminator - genetics ; Computational Biology ; Evolution, Molecular ; Genetic Code ; Genome ; Genomic Library ; Humans ; Internet ; Life Sciences ; Lipoproteins - genetics ; Mice ; Molecular Sequence Data ; Mutation - genetics ; Mycoplasma - genetics ; Mycoplasma - immunology ; Mycoplasma - pathogenicity ; Mycoplasma pulmonis ; Open Reading Frames - genetics ; Polymorphism, Genetic - genetics ; Recombination, Genetic - genetics ; Repetitive Sequences, Nucleic Acid - genetics ; Replication Origin - genetics ; Respiratory System - microbiology ; RNA, Bacterial - genetics ; Virulence - genetics</subject><ispartof>Nucleic acids research, 2001-05, Vol.29 (10), p.2145-2153</ispartof><rights>Copyright Oxford University Press(England) May 15, 2001</rights><rights>Attribution - NonCommercial</rights><rights>Copyright © 2001 Oxford University Press 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-a0a8d5bdf5c5dd753b2c402bb3a3a1aad5fb410b6a607895c18bb71614c7a9ce3</citedby><orcidid>0000-0003-4223-5185 ; 0000-0002-8162-3343 ; 0000-0001-7704-822X ; 0000-0002-1329-7777</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC55444/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC55444/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11353084$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02679458$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Chambaud, I</creatorcontrib><creatorcontrib>Heilig, R</creatorcontrib><creatorcontrib>Ferris, S</creatorcontrib><creatorcontrib>Barbe, V</creatorcontrib><creatorcontrib>Samson, D</creatorcontrib><creatorcontrib>Galisson, F</creatorcontrib><creatorcontrib>Moszer, I</creatorcontrib><creatorcontrib>Dybvig, K</creatorcontrib><creatorcontrib>Wróblewski, H</creatorcontrib><creatorcontrib>Viari, A</creatorcontrib><creatorcontrib>Rocha, E P</creatorcontrib><creatorcontrib>Blanchard, A</creatorcontrib><title>The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>Mycoplasma pulmonis is a wall-less eubacterium belonging to the Mollicutes (trivial name, mycoplasmas) and responsible for murine respiratory diseases. The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glyco-protease are predicted virulence factors. Surprisingly, several of the genes previously reported to be essential for a self-replicating minimal cell are missing in the M.pulmonis genome although this one is larger than the other mycoplasma genomes fully sequenced until now.</description><subject>Animals</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Bacterial - immunology</subject><subject>Base Composition</subject><subject>Biochemistry</subject><subject>Biochemistry, Molecular Biology</subject><subject>Codon, Terminator - genetics</subject><subject>Computational Biology</subject><subject>Evolution, Molecular</subject><subject>Genetic Code</subject><subject>Genome</subject><subject>Genomic Library</subject><subject>Humans</subject><subject>Internet</subject><subject>Life Sciences</subject><subject>Lipoproteins - genetics</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Mutation - genetics</subject><subject>Mycoplasma - genetics</subject><subject>Mycoplasma - immunology</subject><subject>Mycoplasma - pathogenicity</subject><subject>Mycoplasma pulmonis</subject><subject>Open Reading Frames - genetics</subject><subject>Polymorphism, Genetic - genetics</subject><subject>Recombination, Genetic - genetics</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Replication Origin - genetics</subject><subject>Respiratory System - microbiology</subject><subject>RNA, Bacterial - genetics</subject><subject>Virulence - genetics</subject><issn>1362-4962</issn><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EoqVw5YgsDpV62K3HX3EkLlVFKdIiDpSzNXacbqokDnZSaf97vNoVlF44eez5vbH9HiHvga2B1eJyxHTJ61KvOUj1gpyC0Hwla81fPqlPyJucHxgDCUq-JicAQglm5Cn5cbcN1Mdh6sMc6H0Y4xBoDr-WMPpAY0vn0h-W1I2BppCnLuEc045OOG9jwem3nY9Tj3lAOi39EMcuvyWvWuxzeHdcz8jPm89317erzfcvX6-vNisvKz2vkKFplGta5VXTVEo47iXjzgkUCIiNap0E5jRqVplaeTDOVaBB-gprH8QZ-XSYOy1uCI0P45ywt1PqBkw7G7Gz_3bGbmvv46NVSkpZ5BcH-faZ6PZqY_dnjOuqlso8QmHPj1elWLzJsx267EPf4xjikm3FjDai_OF_IBjgkoEp4Mdn4ENc0lj8spwxLZTUVYHWB8inmHMK7Z93ArP7_G3J3_J6v93nXwQfnlryFz8GLn4DkCytWQ</recordid><startdate>20010515</startdate><enddate>20010515</enddate><creator>Chambaud, I</creator><creator>Heilig, R</creator><creator>Ferris, S</creator><creator>Barbe, V</creator><creator>Samson, D</creator><creator>Galisson, F</creator><creator>Moszer, I</creator><creator>Dybvig, K</creator><creator>Wróblewski, H</creator><creator>Viari, A</creator><creator>Rocha, E P</creator><creator>Blanchard, A</creator><general>Oxford Publishing Limited (England)</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-4223-5185</orcidid><orcidid>https://orcid.org/0000-0002-8162-3343</orcidid><orcidid>https://orcid.org/0000-0001-7704-822X</orcidid><orcidid>https://orcid.org/0000-0002-1329-7777</orcidid></search><sort><creationdate>20010515</creationdate><title>The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis</title><author>Chambaud, I ; 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The genome of strain UAB CTIP is composed of a single circular 963 879 bp chromosome with a G + C content of 26.6 mol%, i.e. the lowest reported among bacteria, Ureaplasma urealyticum apart. This genome contains 782 putative coding sequences (CDSs) covering 91.4% of its length and a function could be assigned to 486 CDSs whilst 92 matched the gene sequences of hypothetical proteins, leaving 204 CDSs without significant database match. The genome contains a single set of rRNA genes and only 29 tRNAs genes. The replication origin oriC was localized by sequence analysis and by using the G + C skew method. Sequence polymorphisms within stretches of repeated nucleotides generate phase-variable protein antigens whilst a recombinase gene is likely to catalyse the site-specific DNA inversions in major M.pulmonis surface antigens. Furthermore, a hemolysin, secreted nucleases and a glyco-protease are predicted virulence factors. 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| subjects | Animals Antigens, Bacterial - genetics Antigens, Bacterial - immunology Base Composition Biochemistry Biochemistry, Molecular Biology Codon, Terminator - genetics Computational Biology Evolution, Molecular Genetic Code Genome Genomic Library Humans Internet Life Sciences Lipoproteins - genetics Mice Molecular Sequence Data Mutation - genetics Mycoplasma - genetics Mycoplasma - immunology Mycoplasma - pathogenicity Mycoplasma pulmonis Open Reading Frames - genetics Polymorphism, Genetic - genetics Recombination, Genetic - genetics Repetitive Sequences, Nucleic Acid - genetics Replication Origin - genetics Respiratory System - microbiology RNA, Bacterial - genetics Virulence - genetics |
| title | The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis |
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