Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer
Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multipl...
Gespeichert in:
Veröffentlicht in: | Oncotarget 2017-07, Vol.8 (28), p.45459-45469 |
---|---|
Hauptverfasser: | , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 45469 |
---|---|
container_issue | 28 |
container_start_page | 45459 |
container_title | Oncotarget |
container_volume | 8 |
creator | Zhang, Yixiang Yuan, Yeqing Liang, Pei Zhang, Zhaoxia Guo, Xiaojing Xia, Ligang Zhao, Yingying Shu, Xing-Sheng Sun, Shengkun Ying, Ying Cheng, Yingduan |
description | Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multiple PCa cell lines and primary PCa tissues. Knockdown of KIF14 in DU145 and PC3 prostate cancer cells suppressed cell proliferation, induced cell cycle arrest and apoptosis. Transcriptome analysis by RNA-sequencing demonstrated that KIF4 suppression led to transcriptional changes of genes involved in p53 and TGF-beta signaling pathway. In addition, upregulated expression of GADD45A, GADD45B, p21, PIDD and Shisa5, which contribute to growth arrest and apoptosis induction, and downregulated CCNB1 that promotes cell cycle progression were confirmed by quantitative real-time PCR after KIF4 knockdown. We further found that KIF14 protein level was positively correlated with T stage and Gleason Score. Patients with higher KIF14 expression had shorter overall survival time than those with lower KIF14 expression. Thus, our data indicate that KIF14 could act as a potential oncogene that contributes to tumor progression and poor prognosis in PCa, which may represent a novel and useful prognostic biomarker for PCa. |
doi_str_mv | 10.18632/oncotarget.17564 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5542200</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1900834239</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-359f5ce4a3070648e5e1eca33a3b706843932e8c0b0b07a6bf5a3cb471561e013</originalsourceid><addsrcrecordid>eNpVkctOQyEQhonRaFN9ADeGpZsq13PZmJjGqtGkG10TDp1TMadwBFovTy_VqhUWwMz83wz5ETqm5IxWBWfn3hmfdJhDOqOlLMQOGtBa1CMmJd_duh-goxifSV5SlBWr99EBqySTvGQD9DFdQYC3PkCM1jvsW6yx8yvosNFuZmc6AV53moMDfHc7oQIbHwJ0ORHxq01POC0XPuA--PkPJStx7zdB56ON2Lr1I6Y1L5MNhEO01-ouwtHmHKLHydXD-GZ0P72-HV_ejwyXRRpxWbfSgNCclKQQFUigYDTnmjc5UAlecwaVIU3epS6aVmpuGlFSWVAglA_RxTe3XzYLmBlwKehO9cEudHhXXlv1P-Psk5r7lZJSMEZIBpxuAMG_LCEmtbDRQNdpB34ZFa0JqbhgeZAhot-lJv81Bmh_21CivmxTf7apL9uy5mR7vl_Fj0n8EztumXQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1900834239</pqid></control><display><type>article</type><title>Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free E- Journals</source><creator>Zhang, Yixiang ; Yuan, Yeqing ; Liang, Pei ; Zhang, Zhaoxia ; Guo, Xiaojing ; Xia, Ligang ; Zhao, Yingying ; Shu, Xing-Sheng ; Sun, Shengkun ; Ying, Ying ; Cheng, Yingduan</creator><creatorcontrib>Zhang, Yixiang ; Yuan, Yeqing ; Liang, Pei ; Zhang, Zhaoxia ; Guo, Xiaojing ; Xia, Ligang ; Zhao, Yingying ; Shu, Xing-Sheng ; Sun, Shengkun ; Ying, Ying ; Cheng, Yingduan</creatorcontrib><description>Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multiple PCa cell lines and primary PCa tissues. Knockdown of KIF14 in DU145 and PC3 prostate cancer cells suppressed cell proliferation, induced cell cycle arrest and apoptosis. Transcriptome analysis by RNA-sequencing demonstrated that KIF4 suppression led to transcriptional changes of genes involved in p53 and TGF-beta signaling pathway. In addition, upregulated expression of GADD45A, GADD45B, p21, PIDD and Shisa5, which contribute to growth arrest and apoptosis induction, and downregulated CCNB1 that promotes cell cycle progression were confirmed by quantitative real-time PCR after KIF4 knockdown. We further found that KIF14 protein level was positively correlated with T stage and Gleason Score. Patients with higher KIF14 expression had shorter overall survival time than those with lower KIF14 expression. Thus, our data indicate that KIF14 could act as a potential oncogene that contributes to tumor progression and poor prognosis in PCa, which may represent a novel and useful prognostic biomarker for PCa.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.17564</identifier><identifier>PMID: 28525372</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Adult ; Aged ; Apoptosis - genetics ; Biomarkers, Tumor ; Cell Line, Tumor ; Cell Proliferation ; Computational Biology - methods ; Disease Progression ; G2 Phase Cell Cycle Checkpoints - genetics ; Gene Expression ; Gene Expression Profiling ; Gene Knockdown Techniques ; Humans ; Kinesin - genetics ; Male ; Middle Aged ; Neoplasm Grading ; Neoplasm Staging ; Oncogene Proteins - genetics ; Oncogenes ; Prognosis ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - mortality ; Prostatic Neoplasms - pathology ; Research Paper</subject><ispartof>Oncotarget, 2017-07, Vol.8 (28), p.45459-45469</ispartof><rights>Copyright: © 2017 Zhang et al. 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-359f5ce4a3070648e5e1eca33a3b706843932e8c0b0b07a6bf5a3cb471561e013</citedby><cites>FETCH-LOGICAL-c356t-359f5ce4a3070648e5e1eca33a3b706843932e8c0b0b07a6bf5a3cb471561e013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542200/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5542200/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28525372$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yixiang</creatorcontrib><creatorcontrib>Yuan, Yeqing</creatorcontrib><creatorcontrib>Liang, Pei</creatorcontrib><creatorcontrib>Zhang, Zhaoxia</creatorcontrib><creatorcontrib>Guo, Xiaojing</creatorcontrib><creatorcontrib>Xia, Ligang</creatorcontrib><creatorcontrib>Zhao, Yingying</creatorcontrib><creatorcontrib>Shu, Xing-Sheng</creatorcontrib><creatorcontrib>Sun, Shengkun</creatorcontrib><creatorcontrib>Ying, Ying</creatorcontrib><creatorcontrib>Cheng, Yingduan</creatorcontrib><title>Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multiple PCa cell lines and primary PCa tissues. Knockdown of KIF14 in DU145 and PC3 prostate cancer cells suppressed cell proliferation, induced cell cycle arrest and apoptosis. Transcriptome analysis by RNA-sequencing demonstrated that KIF4 suppression led to transcriptional changes of genes involved in p53 and TGF-beta signaling pathway. In addition, upregulated expression of GADD45A, GADD45B, p21, PIDD and Shisa5, which contribute to growth arrest and apoptosis induction, and downregulated CCNB1 that promotes cell cycle progression were confirmed by quantitative real-time PCR after KIF4 knockdown. We further found that KIF14 protein level was positively correlated with T stage and Gleason Score. Patients with higher KIF14 expression had shorter overall survival time than those with lower KIF14 expression. Thus, our data indicate that KIF14 could act as a potential oncogene that contributes to tumor progression and poor prognosis in PCa, which may represent a novel and useful prognostic biomarker for PCa.</description><subject>Adult</subject><subject>Aged</subject><subject>Apoptosis - genetics</subject><subject>Biomarkers, Tumor</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Computational Biology - methods</subject><subject>Disease Progression</subject><subject>G2 Phase Cell Cycle Checkpoints - genetics</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Kinesin - genetics</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Staging</subject><subject>Oncogene Proteins - genetics</subject><subject>Oncogenes</subject><subject>Prognosis</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - mortality</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Research Paper</subject><issn>1949-2553</issn><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctOQyEQhonRaFN9ADeGpZsq13PZmJjGqtGkG10TDp1TMadwBFovTy_VqhUWwMz83wz5ETqm5IxWBWfn3hmfdJhDOqOlLMQOGtBa1CMmJd_duh-goxifSV5SlBWr99EBqySTvGQD9DFdQYC3PkCM1jvsW6yx8yvosNFuZmc6AV53moMDfHc7oQIbHwJ0ORHxq01POC0XPuA--PkPJStx7zdB56ON2Lr1I6Y1L5MNhEO01-ouwtHmHKLHydXD-GZ0P72-HV_ejwyXRRpxWbfSgNCclKQQFUigYDTnmjc5UAlecwaVIU3epS6aVmpuGlFSWVAglA_RxTe3XzYLmBlwKehO9cEudHhXXlv1P-Psk5r7lZJSMEZIBpxuAMG_LCEmtbDRQNdpB34ZFa0JqbhgeZAhot-lJv81Bmh_21CivmxTf7apL9uy5mR7vl_Fj0n8EztumXQ</recordid><startdate>20170711</startdate><enddate>20170711</enddate><creator>Zhang, Yixiang</creator><creator>Yuan, Yeqing</creator><creator>Liang, Pei</creator><creator>Zhang, Zhaoxia</creator><creator>Guo, Xiaojing</creator><creator>Xia, Ligang</creator><creator>Zhao, Yingying</creator><creator>Shu, Xing-Sheng</creator><creator>Sun, Shengkun</creator><creator>Ying, Ying</creator><creator>Cheng, Yingduan</creator><general>Impact Journals LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170711</creationdate><title>Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer</title><author>Zhang, Yixiang ; Yuan, Yeqing ; Liang, Pei ; Zhang, Zhaoxia ; Guo, Xiaojing ; Xia, Ligang ; Zhao, Yingying ; Shu, Xing-Sheng ; Sun, Shengkun ; Ying, Ying ; Cheng, Yingduan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-359f5ce4a3070648e5e1eca33a3b706843932e8c0b0b07a6bf5a3cb471561e013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Apoptosis - genetics</topic><topic>Biomarkers, Tumor</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Computational Biology - methods</topic><topic>Disease Progression</topic><topic>G2 Phase Cell Cycle Checkpoints - genetics</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>Kinesin - genetics</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Staging</topic><topic>Oncogene Proteins - genetics</topic><topic>Oncogenes</topic><topic>Prognosis</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - mortality</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Research Paper</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yixiang</creatorcontrib><creatorcontrib>Yuan, Yeqing</creatorcontrib><creatorcontrib>Liang, Pei</creatorcontrib><creatorcontrib>Zhang, Zhaoxia</creatorcontrib><creatorcontrib>Guo, Xiaojing</creatorcontrib><creatorcontrib>Xia, Ligang</creatorcontrib><creatorcontrib>Zhao, Yingying</creatorcontrib><creatorcontrib>Shu, Xing-Sheng</creatorcontrib><creatorcontrib>Sun, Shengkun</creatorcontrib><creatorcontrib>Ying, Ying</creatorcontrib><creatorcontrib>Cheng, Yingduan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yixiang</au><au>Yuan, Yeqing</au><au>Liang, Pei</au><au>Zhang, Zhaoxia</au><au>Guo, Xiaojing</au><au>Xia, Ligang</au><au>Zhao, Yingying</au><au>Shu, Xing-Sheng</au><au>Sun, Shengkun</au><au>Ying, Ying</au><au>Cheng, Yingduan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2017-07-11</date><risdate>2017</risdate><volume>8</volume><issue>28</issue><spage>45459</spage><epage>45469</epage><pages>45459-45469</pages><issn>1949-2553</issn><eissn>1949-2553</eissn><abstract>Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multiple PCa cell lines and primary PCa tissues. Knockdown of KIF14 in DU145 and PC3 prostate cancer cells suppressed cell proliferation, induced cell cycle arrest and apoptosis. Transcriptome analysis by RNA-sequencing demonstrated that KIF4 suppression led to transcriptional changes of genes involved in p53 and TGF-beta signaling pathway. In addition, upregulated expression of GADD45A, GADD45B, p21, PIDD and Shisa5, which contribute to growth arrest and apoptosis induction, and downregulated CCNB1 that promotes cell cycle progression were confirmed by quantitative real-time PCR after KIF4 knockdown. We further found that KIF14 protein level was positively correlated with T stage and Gleason Score. Patients with higher KIF14 expression had shorter overall survival time than those with lower KIF14 expression. Thus, our data indicate that KIF14 could act as a potential oncogene that contributes to tumor progression and poor prognosis in PCa, which may represent a novel and useful prognostic biomarker for PCa.</abstract><cop>United States</cop><pub>Impact Journals LLC</pub><pmid>28525372</pmid><doi>10.18632/oncotarget.17564</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1949-2553 |
ispartof | Oncotarget, 2017-07, Vol.8 (28), p.45459-45469 |
issn | 1949-2553 1949-2553 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5542200 |
source | MEDLINE; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free E- Journals |
subjects | Adult Aged Apoptosis - genetics Biomarkers, Tumor Cell Line, Tumor Cell Proliferation Computational Biology - methods Disease Progression G2 Phase Cell Cycle Checkpoints - genetics Gene Expression Gene Expression Profiling Gene Knockdown Techniques Humans Kinesin - genetics Male Middle Aged Neoplasm Grading Neoplasm Staging Oncogene Proteins - genetics Oncogenes Prognosis Prostatic Neoplasms - genetics Prostatic Neoplasms - mortality Prostatic Neoplasms - pathology Research Paper |
title | Overexpression of a novel candidate oncogene KIF14 correlates with tumor progression and poor prognosis in prostate cancer |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T18%3A03%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Overexpression%20of%20a%20novel%20candidate%20oncogene%20KIF14%20correlates%20with%20tumor%20progression%20and%20poor%20prognosis%20in%20prostate%20cancer&rft.jtitle=Oncotarget&rft.au=Zhang,%20Yixiang&rft.date=2017-07-11&rft.volume=8&rft.issue=28&rft.spage=45459&rft.epage=45469&rft.pages=45459-45469&rft.issn=1949-2553&rft.eissn=1949-2553&rft_id=info:doi/10.18632/oncotarget.17564&rft_dat=%3Cproquest_pubme%3E1900834239%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1900834239&rft_id=info:pmid/28525372&rfr_iscdi=true |