A toolkit for GFP-mediated tissue-specific protein degradation in C. elegans

Proteins that are essential for embryo production, cell division and early embryonic events are frequently reused later in embryogenesis, during organismal development or in the adult. Examining protein function across these different biological contexts requires tissue-specific perturbation. Here,...

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Veröffentlicht in:	Development (Cambridge) 2017-07, Vol.144 (14), p.2694-2701
Hauptverfasser:	Wang, Shaohe, Tang, Ngang Heok, Lara-Gonzalez, Pablo, Zhao, Zhiling, Cheerambathur, Dhanya K, Prevo, Bram, Chisholm, Andrew D, Desai, Arshad, Oegema, Karen
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Genetically Modified Axons - metabolism Body wall Caenorhabditis elegans - genetics Caenorhabditis elegans - growth & development Caenorhabditis elegans - metabolism Caenorhabditis elegans Proteins - genetics Caenorhabditis elegans Proteins - metabolism Carrier Proteins - genetics Carrier Proteins - metabolism Cell division CRISPR Embryogenesis Epidermis Genes, Helminth Genetic Techniques Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Intestine MAP Kinase Kinase Kinases - genetics MAP Kinase Kinase Kinases - metabolism Mutation Nanobodies Nematodes Proteasomes Proteins Proteolysis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sensory neurons Single-Domain Antibodies - genetics Single-Domain Antibodies - metabolism Techniques and Resources Tissue Distribution Ubiquitin Ubiquitin-protein ligase
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