Fast volumetric calcium imaging across multiple cortical layers using sculpted light

Two-photon scanning microscopy is inherently slow and thus limits volumetric calcium imaging. Prevedel et al. achieve increased volumetric imaging speed by tailoring the excitation volume via light sculpting. Although whole-organism calcium imaging in small and semi-transparent animals has been demo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nature methods 2016-12, Vol.13 (12), p.1021-1028
Hauptverfasser:	Prevedel, Robert, Verhoef, Aart J, Pernía-Andrade, Alejandro J, Weisenburger, Siegfried, Huang, Ben S, Nöbauer, Tobias, Fernández, Alma, Delcour, Jeroen E, Golshani, Peyman, Baltuska, Andrius, Vaziri, Alipasha
Format:	Artikel
Sprache:	eng
Schlagworte:	14/35 14/63 14/69 631/1647/245/2225 631/1647/328/2057 631/1647/328/2235 631/1647/334/1874/345 631/378 64/110 Analysis Animals Behavior, Animal - physiology Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology Calcium Calcium - metabolism Calcium ions Calcium Signaling - physiology Cerebral Cortex - metabolism Hippocampus - metabolism Lasers Life Sciences Light Mammals Medical imaging Mice Microscopy Microscopy, Confocal Molecular Imaging - methods Neurons Neurons - metabolism Photons Physiological aspects Proteomics Time Factors Volumetric analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!