Individuals with obesity and type 2 diabetes have additional immune dysfunction compared with obese individuals who are metabolically healthy

Individuals with obesity and type 2 diabetes have additional immune dysfunction compared with obese individuals who are metabolically healthy

ObjectiveThe objective of the current study was to compare the responses to different ex vivo immunogenic challenges between immune cells derived from metabolically healthy subjects with obesity and subjects with obesity and type 2 diabetes.Research design and methodsWe recruited 10 metabolically he...

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Veröffentlicht in:BMJ open diabetes research & care 2017-05, Vol.5 (1), p.e000379-e000379
Hauptverfasser: Richard, Caroline, Wadowski, Michael, Goruk, Susan, Cameron, Lisa, Sharma, Arya M, Field, Catherine J
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Immunology and Transplantation
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container_issue 1
container_start_page e000379
container_title BMJ open diabetes research & care
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creator Richard, Caroline
Wadowski, Michael
Goruk, Susan
Cameron, Lisa
Sharma, Arya M
Field, Catherine J
description ObjectiveThe objective of the current study was to compare the responses to different ex vivo immunogenic challenges between immune cells derived from metabolically healthy subjects with obesity and subjects with obesity and type 2 diabetes.Research design and methodsWe recruited 10 metabolically healthy subjects with obesity (Edmonton Obesity Staging System (EOSS) stage 0) and 9 subjects with obesity and type 2 diabetes (EOSS stage 2) aged between 21 years and 70 years and matched for body mass index. Peripheral blood mononuclear cells (PBMCs) were isolated and immune cell phenotypes and ex vivo cytokine production after phytohaemagglutinin (PHA, a T cell mitogen) stimulation were determined. Neutrophil oxidative burst activity was assessed in whole blood.ResultsPBMCs from subjects with stage 2 obesity produced significantly less interleukin (IL)-2, IL-6 and tumour necrosis factor α after PHA stimulation than PBMCs from subjects with stage 0 obesity (all, p
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Peripheral blood mononuclear cells (PBMCs) were isolated and immune cell phenotypes and ex vivo cytokine production after phytohaemagglutinin (PHA, a T cell mitogen) stimulation were determined. Neutrophil oxidative burst activity was assessed in whole blood.ResultsPBMCs from subjects with stage 2 obesity produced significantly less interleukin (IL)-2, IL-6 and tumour necrosis factor α after PHA stimulation than PBMCs from subjects with stage 0 obesity (all, p&lt;0.05). Subjects with stage 2 obesity also had higher proportions of cytotoxic T cells, activated helper T cells (CD4+CD278+) and inflammatory monocytes (CD14+CRTh2+, all p&lt;0.05). Poststimulation, neutrophils from subjects with stage 2 obesity produced significantly more free radicals, were larger and more granular and had a lower stimulation index (all p&lt;0.05).ConclusionsOur results suggest that compared with obese individuals metabolically healthy individuals with obesity and type 2 diabetes have an impaired neutrophil function and T cell response on challenge despite having a T cell population expressing more activation markers which may be partly responsible for the increased prevalence of infection reported in this population.</description><identifier>ISSN: 2052-4897</identifier><identifier>EISSN: 2052-4897</identifier><identifier>DOI: 10.1136/bmjdrc-2016-000379</identifier><identifier>PMID: 28761653</identifier><language>eng</language><publisher>England: BMJ Publishing Group LTD</publisher><subject>Immunology and Transplantation</subject><ispartof>BMJ open diabetes research &amp; care, 2017-05, Vol.5 (1), p.e000379-e000379</ispartof><rights>Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.</rights><rights>Copyright: 2017 (c) Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted.</rights><rights>Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No commercial use is permitted unless otherwise expressly granted. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b468t-501d2848e4d7f89fbc85075168ec5eb0bb0b136c634530b520ff36b92280ce6d3</citedby><cites>FETCH-LOGICAL-b468t-501d2848e4d7f89fbc85075168ec5eb0bb0b136c634530b520ff36b92280ce6d3</cites><orcidid>0000-0003-1775-6464</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttp://drc.bmj.com/content/5/1/e000379.full.pdf$$EPDF$$P50$$Gbmj$$Hfree_for_read</linktopdf><linktohtml>$$Uhttp://drc.bmj.com/content/5/1/e000379.full$$EHTML$$P50$$Gbmj$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27549,27550,27924,27925,53791,53793,77601,77632</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28761653$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Richard, Caroline</creatorcontrib><creatorcontrib>Wadowski, Michael</creatorcontrib><creatorcontrib>Goruk, Susan</creatorcontrib><creatorcontrib>Cameron, Lisa</creatorcontrib><creatorcontrib>Sharma, Arya M</creatorcontrib><creatorcontrib>Field, Catherine J</creatorcontrib><title>Individuals with obesity and type 2 diabetes have additional immune dysfunction compared with obese individuals who are metabolically healthy</title><title>BMJ open diabetes research &amp; care</title><addtitle>BMJ Open Diabetes Res Care</addtitle><description>ObjectiveThe objective of the current study was to compare the responses to different ex vivo immunogenic challenges between immune cells derived from metabolically healthy subjects with obesity and subjects with obesity and type 2 diabetes.Research design and methodsWe recruited 10 metabolically healthy subjects with obesity (Edmonton Obesity Staging System (EOSS) stage 0) and 9 subjects with obesity and type 2 diabetes (EOSS stage 2) aged between 21 years and 70 years and matched for body mass index. Peripheral blood mononuclear cells (PBMCs) were isolated and immune cell phenotypes and ex vivo cytokine production after phytohaemagglutinin (PHA, a T cell mitogen) stimulation were determined. Neutrophil oxidative burst activity was assessed in whole blood.ResultsPBMCs from subjects with stage 2 obesity produced significantly less interleukin (IL)-2, IL-6 and tumour necrosis factor α after PHA stimulation than PBMCs from subjects with stage 0 obesity (all, p&lt;0.05). Subjects with stage 2 obesity also had higher proportions of cytotoxic T cells, activated helper T cells (CD4+CD278+) and inflammatory monocytes (CD14+CRTh2+, all p&lt;0.05). Poststimulation, neutrophils from subjects with stage 2 obesity produced significantly more free radicals, were larger and more granular and had a lower stimulation index (all p&lt;0.05).ConclusionsOur results suggest that compared with obese individuals metabolically healthy individuals with obesity and type 2 diabetes have an impaired neutrophil function and T cell response on challenge despite having a T cell population expressing more activation markers which may be partly responsible for the increased prevalence of infection reported in this population.</description><subject>Immunology and Transplantation</subject><issn>2052-4897</issn><issn>2052-4897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>9YT</sourceid><sourceid>ACMMV</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNkctq3DAUhk1paEKSF-iiCLrpxq0ulixvCiX0Egh0k6yFLse1BtmaWvIUP0TfuRqcptOuCgIJne__j47-qnpJ8FtCmHhnxp2bbU0xETXGmLXds-qCYk7rRnbt85PzeXWd0q4wRUaY5C-qcypbQQRnF9XP28n5g3eLDgn98HlA0UDyeUV6ciive0AUOa8NZEho0AdA2jmffZx0QH4clwmQW1O_TPZ4iWwc93oG98cMkD_tMURU6miErE0M3uoQVjSADnlYr6qzvkBw_bhfVg-fPt7ffKnvvn6-vflwV5tGyFxzTByVjYTGtb3semMlxy0nQoLlYLApqwxrBWs4w4ZT3PdMmI5SiS0Ixy6r95vvfjEjOAtTnnVQ-9mPel5V1F79XZn8oL7Fg-LFj3JaDN48Gszx-wIpq9EnCyHoCeKSFOkKJVvGWUFf_4Pu4jKX3yuU7AQlkpGmUHSj7BxTmqF_egzB6hi42gJXx8DVFngRvTod40nyO94C1BtQxP9j-Asairly</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Richard, Caroline</creator><creator>Wadowski, Michael</creator><creator>Goruk, Susan</creator><creator>Cameron, Lisa</creator><creator>Sharma, Arya M</creator><creator>Field, Catherine J</creator><general>BMJ Publishing Group LTD</general><general>BMJ Open Diabetes Research &amp; 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care</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Richard, Caroline</au><au>Wadowski, Michael</au><au>Goruk, Susan</au><au>Cameron, Lisa</au><au>Sharma, Arya M</au><au>Field, Catherine J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Individuals with obesity and type 2 diabetes have additional immune dysfunction compared with obese individuals who are metabolically healthy</atitle><jtitle>BMJ open diabetes research &amp; care</jtitle><addtitle>BMJ Open Diabetes Res Care</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>5</volume><issue>1</issue><spage>e000379</spage><epage>e000379</epage><pages>e000379-e000379</pages><issn>2052-4897</issn><eissn>2052-4897</eissn><abstract>ObjectiveThe objective of the current study was to compare the responses to different ex vivo immunogenic challenges between immune cells derived from metabolically healthy subjects with obesity and subjects with obesity and type 2 diabetes.Research design and methodsWe recruited 10 metabolically healthy subjects with obesity (Edmonton Obesity Staging System (EOSS) stage 0) and 9 subjects with obesity and type 2 diabetes (EOSS stage 2) aged between 21 years and 70 years and matched for body mass index. Peripheral blood mononuclear cells (PBMCs) were isolated and immune cell phenotypes and ex vivo cytokine production after phytohaemagglutinin (PHA, a T cell mitogen) stimulation were determined. Neutrophil oxidative burst activity was assessed in whole blood.ResultsPBMCs from subjects with stage 2 obesity produced significantly less interleukin (IL)-2, IL-6 and tumour necrosis factor α after PHA stimulation than PBMCs from subjects with stage 0 obesity (all, p&lt;0.05). Subjects with stage 2 obesity also had higher proportions of cytotoxic T cells, activated helper T cells (CD4+CD278+) and inflammatory monocytes (CD14+CRTh2+, all p&lt;0.05). Poststimulation, neutrophils from subjects with stage 2 obesity produced significantly more free radicals, were larger and more granular and had a lower stimulation index (all p&lt;0.05).ConclusionsOur results suggest that compared with obese individuals metabolically healthy individuals with obesity and type 2 diabetes have an impaired neutrophil function and T cell response on challenge despite having a T cell population expressing more activation markers which may be partly responsible for the increased prevalence of infection reported in this population.</abstract><cop>England</cop><pub>BMJ Publishing Group LTD</pub><pmid>28761653</pmid><doi>10.1136/bmjdrc-2016-000379</doi><orcidid>https://orcid.org/0000-0003-1775-6464</orcidid><oa>free_for_read</oa></addata></record>
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subjects Immunology and Transplantation
title Individuals with obesity and type 2 diabetes have additional immune dysfunction compared with obese individuals who are metabolically healthy
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