Nucleic acid detection with CRISPR-Cas13a/C2c2

Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a “collateral ef...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2017-04, Vol.356 (6336), p.438-442
Hauptverfasser:	Gootenberg, Jonathan S., Abudayyeh, Omar O., Lee, Jeong Wook, Essletzbichler, Patrick, Dy, Aaron J., Joung, Julia, Verdine, Vanessa, Donghia, Nina, Daringer, Nichole M., Freije, Catherine A., Myhrvold, Cameron, Bhattacharyya, Roby P., Livny, Jonathan, Regev, Aviv, Koonin, Eugene V., Hung, Deborah T., Sabeti, Pardis C., Collins, James J., Zhang, Feng
Format:	Artikel
Sprache:	eng
Schlagworte:	Activity recognition Amplification Bacteria Bacteria - isolation & purification Bacteria - pathogenicity Bacterial Proteins - chemistry Circulating Tumor DNA - analysis Circulating Tumor DNA - genetics Clustered Regularly Interspaced Short Palindromic Repeats CRISPR Dengue - diagnosis Dengue fever Dengue Virus - genetics Dengue Virus - isolation & purification Deoxyribonucleic acid DNA DNA, Bacterial - analysis Flaviviridae Gene sequencing Genotyping Human performance Humans Mutation Neoplasms - genetics Nucleic acids Nucleotide sequence Pathogens Point-of-Care Systems Reagents Recombinase Ribonuclease Ribonucleases - chemistry Ribonucleic acid Ribonucleic acids RNA RNA Cleavage RNA, Viral - analysis RNA, Viral - genetics Sensitivity Target recognition Vector-borne diseases Viruses Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus Infection - diagnosis
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