Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses
The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2017-01, Vol.2017 (2017), p.1-9 |
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| creator | Wang, Zheng-Tao Tsim, Karl Wah-Keung Gong, Amy G. W. Wu, Lei-Lei Lu, Ye Zhang, Cheng-Gang Dai, Jia-Rong Ye, Zi Xu, Hong |
| description | The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D. officinale and D. devonianum by comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage mass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1 lignan in D. officinale. Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D. officinale, and violanthin and isoviolanthin were identified to be specific for D. officinale compared with D. devonianum. Then, two representative components were used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied in distinguishing D. officinale from D. devonianum. The results of this work have demonstrated that these developed analytical methods can be used to discriminate D. officinale and D. devonianum effectively and conveniently. |
| doi_str_mv | 10.1155/2017/8647212 |
| format | Article |
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W. ; Wu, Lei-Lei ; Lu, Ye ; Zhang, Cheng-Gang ; Dai, Jia-Rong ; Ye, Zi ; Xu, Hong</creator><contributor>Tomczyk, Michał</contributor><creatorcontrib>Wang, Zheng-Tao ; Tsim, Karl Wah-Keung ; Gong, Amy G. W. ; Wu, Lei-Lei ; Lu, Ye ; Zhang, Cheng-Gang ; Dai, Jia-Rong ; Ye, Zi ; Xu, Hong ; Tomczyk, Michał</creatorcontrib><description>The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D. officinale and D. devonianum by comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage mass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1 lignan in D. officinale. Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D. officinale, and violanthin and isoviolanthin were identified to be specific for D. officinale compared with D. devonianum. Then, two representative components were used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied in distinguishing D. officinale from D. devonianum. The results of this work have demonstrated that these developed analytical methods can be used to discriminate D. officinale and D. devonianum effectively and conveniently.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2017/8647212</identifier><identifier>PMID: 28769988</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Analysis ; Cancer ; Chemicals ; Chinese medicine ; Flavonoids ; High performance liquid chromatography ; Identification ; Isoflavones ; Laboratories ; Medicine, Chinese ; Morphology ; Quality control ; R&D ; Research & development</subject><ispartof>Evidence-based complementary and alternative medicine, 2017-01, Vol.2017 (2017), p.1-9</ispartof><rights>Copyright © 2017 Zi Ye et al.</rights><rights>COPYRIGHT 2017 John Wiley & Sons, Inc.</rights><rights>Copyright © 2017 Zi Ye et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2017 Zi Ye et al. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-a4d5537e48c4b1f18b298782381c955706a80c0eefc81eb7bf76ddfec8b8e2ae3</citedby><cites>FETCH-LOGICAL-c499t-a4d5537e48c4b1f18b298782381c955706a80c0eefc81eb7bf76ddfec8b8e2ae3</cites><orcidid>0000-0001-6397-1603 ; 0000-0003-4808-1674</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5523191/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5523191/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28769988$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tomczyk, Michał</contributor><creatorcontrib>Wang, Zheng-Tao</creatorcontrib><creatorcontrib>Tsim, Karl Wah-Keung</creatorcontrib><creatorcontrib>Gong, Amy G. W.</creatorcontrib><creatorcontrib>Wu, Lei-Lei</creatorcontrib><creatorcontrib>Lu, Ye</creatorcontrib><creatorcontrib>Zhang, Cheng-Gang</creatorcontrib><creatorcontrib>Dai, Jia-Rong</creatorcontrib><creatorcontrib>Ye, Zi</creatorcontrib><creatorcontrib>Xu, Hong</creatorcontrib><title>Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D. officinale and D. devonianum by comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage mass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1 lignan in D. officinale. Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D. officinale, and violanthin and isoviolanthin were identified to be specific for D. officinale compared with D. devonianum. Then, two representative components were used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied in distinguishing D. officinale from D. devonianum. The results of this work have demonstrated that these developed analytical methods can be used to discriminate D. officinale and D. devonianum effectively and conveniently.</description><subject>Analysis</subject><subject>Cancer</subject><subject>Chemicals</subject><subject>Chinese medicine</subject><subject>Flavonoids</subject><subject>High performance liquid chromatography</subject><subject>Identification</subject><subject>Isoflavones</subject><subject>Laboratories</subject><subject>Medicine, Chinese</subject><subject>Morphology</subject><subject>Quality control</subject><subject>R&D</subject><subject>Research & development</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkk1v1DAQQCMEoqVw44wicUFiQ23Hju0L0mrbspUWUamtxM1ynPGuq8QucVK0v4C_jfeDbeHEyWPP0_OMPVn2FqNPGDN2ShDmp6KinGDyLDvGnOKCEiGeH2L-_Sh7FeMdQkRyzl9mR0TwSkohjrNfsxV0zug2P3PWQg9-cHpwwefB5mfgmz7UbuzSzjrjvG4h1755mmngIXinfQrrdX4bnV_m86vFLL9IEfT3vfNDnGyPivPry-Lr9WSrmF_dJGialOsI8XX2wuo2wpv9epLdXpzfzObF4tuXy9l0URgq5VBo2jBWcqDC0BpbLGoiBRekFNhIxjiqtEAGAVgjMNS8trxqGgtG1AKIhvIk-7zz3o91B41J_fa6VanKTvdrFbRTf2e8W6lleFCMkRJLnAQf9oI-_BghDqpz0UDbag9hjApLwoSQJUcJff8PehfGPjW8pQhFAiP5SC3T4yrnbUj3mo1UTRmrUEUruqEmO8r0IcYe7KFkjNRmDtRmDtR-DhL-7mmbB_jPxyfg4w5YOd_on-4_dZAYsPqRxowKgsrfI7LD-Q</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Wang, Zheng-Tao</creator><creator>Tsim, Karl Wah-Keung</creator><creator>Gong, Amy G. 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W.</au><au>Wu, Lei-Lei</au><au>Lu, Ye</au><au>Zhang, Cheng-Gang</au><au>Dai, Jia-Rong</au><au>Ye, Zi</au><au>Xu, Hong</au><au>Tomczyk, Michał</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>2017</volume><issue>2017</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D. officinale and D. devonianum by comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage mass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1 lignan in D. officinale. Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D. officinale, and violanthin and isoviolanthin were identified to be specific for D. officinale compared with D. devonianum. Then, two representative components were used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied in distinguishing D. officinale from D. devonianum. The results of this work have demonstrated that these developed analytical methods can be used to discriminate D. officinale and D. devonianum effectively and conveniently.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>28769988</pmid><doi>10.1155/2017/8647212</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6397-1603</orcidid><orcidid>https://orcid.org/0000-0003-4808-1674</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Analysis Cancer Chemicals Chinese medicine Flavonoids High performance liquid chromatography Identification Isoflavones Laboratories Medicine, Chinese Morphology Quality control R&D Research & development |
| title | Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses |
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