Cleavage‐site preferences of Sindbis virus polyproteins containing the non‐structural proteinase. Evidence for temporal regulation of polyprotein processing in vivo

The non‐structural proteins of Sindbis virus, nsP1, 2, 3 and 4, are produced upon cleavage of polyproteins P123 and P1234 by a proteinase residing in nsP2. We used cell free translation of SP6 transcripts to study the proteolytic activity of nsP2 and of nsP2‐containing polyproteins. To generate poly...

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Veröffentlicht in:	The EMBO journal 1990-08, Vol.9 (8), p.2631-2638
Hauptverfasser:	Groot, R. J., Hardy, W. R., Shirako, Y., Strauss, J. H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Capsid - genetics Capsid - metabolism Cells, Cultured Chick Embryo Codon - genetics Endopeptidases - metabolism Fibroblasts - enzymology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutation Oligonucleotide Probes Plasmids Polymerase Chain Reaction Protein Biosynthesis Protein Processing, Post-Translational RNA, Viral - genetics Sindbis Virus - enzymology Sindbis Virus - genetics Transcription, Genetic Translation. Translation factors. Protein processing Viral Core Proteins - genetics Viral Core Proteins - metabolism Viral Nonstructural Proteins
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