Illuminating developmental biology through photochemistry
Developmental biology has been continually shaped by technological advances, evolving from a descriptive science into one immersed in molecular and cellular mechanisms. Most recently, genome sequencing and 'omics' profiling have provided developmental biologists with a wealth of genetic an...
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| Veröffentlicht in: | Nature chemical biology 2017-05, Vol.13 (6), p.587-598 |
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| creator | Kowalik, Lukasz Chen, James K |
| description | Developmental biology has been continually shaped by technological advances, evolving from a descriptive science into one immersed in molecular and cellular mechanisms. Most recently, genome sequencing and 'omics' profiling have provided developmental biologists with a wealth of genetic and biochemical information; however, fully translating this knowledge into functional understanding will require new experimental capabilities. Photoactivatable probes have emerged as particularly valuable tools for investigating developmental mechanisms, as they can enable rapid, specific manipulations of DNA, RNA, proteins, and cells with spatiotemporal precision. In this Perspective, we describe optochemical and optogenetic systems that have been applied in multicellular organisms, insights gained through the use of these probes, and their current limitations. We also suggest how chemical biologists can expand the reach of photoactivatable technologies and bring new depth to our understanding of organismal development. |
| doi_str_mv | 10.1038/nchembio.2369 |
| format | Article |
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We also suggest how chemical biologists can expand the reach of photoactivatable technologies and bring new depth to our understanding of organismal development.</description><subject>13/95</subject><subject>14/63</subject><subject>38/70</subject><subject>631/136</subject><subject>631/136/334/1874</subject><subject>631/80/86</subject><subject>631/92/96</subject><subject>64/11</subject><subject>64/110</subject><subject>64/116</subject><subject>64/24</subject><subject>96/95</subject><subject>96/98</subject><subject>Biochemical Engineering</subject><subject>Biochemistry</subject><subject>Biological evolution</subject><subject>Biologists</subject><subject>Biology</subject><subject>Bioorganic Chemistry</subject><subject>Cell Biology</subject><subject>Chemistry</subject><subject>Chemistry/Food Science</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental biology</subject><subject>Developmental Biology - instrumentation</subject><subject>Developmental Biology - methods</subject><subject>Developmental Biology - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nature chemical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kowalik, Lukasz</au><au>Chen, James K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Illuminating developmental biology through photochemistry</atitle><jtitle>Nature chemical biology</jtitle><stitle>Nat Chem Biol</stitle><addtitle>Nat Chem Biol</addtitle><date>2017-05-17</date><risdate>2017</risdate><volume>13</volume><issue>6</issue><spage>587</spage><epage>598</epage><pages>587-598</pages><issn>1552-4450</issn><eissn>1552-4469</eissn><abstract>Developmental biology has been continually shaped by technological advances, evolving from a descriptive science into one immersed in molecular and cellular mechanisms. 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| subjects | 13/95 14/63 38/70 631/136 631/136/334/1874 631/80/86 631/92/96 64/11 64/110 64/116 64/24 96/95 96/98 Biochemical Engineering Biochemistry Biological evolution Biologists Biology Bioorganic Chemistry Cell Biology Chemistry Chemistry/Food Science Deoxyribonucleic acid Developmental biology Developmental Biology - instrumentation Developmental Biology - methods Developmental Biology - trends DNA DNA probes Gene sequencing Genomes Genomics Models, Biological Molecular Probes - metabolism Molecular Structure perspective Photochemistry Photochemistry - trends Probes Proteins Rhodopsin - chemistry Ribonucleic acid RNA |
| title | Illuminating developmental biology through photochemistry |
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