Top-Down Proteomics of Large Proteins up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy

Mass spectrometry (MS)-based top-down proteomics is a powerful method for the comprehensive analysis of proteoforms that arise from genetic variations and post-translational modifications (PTMs). However, top-down MS analysis of high molecular weight (MW) proteins remains challenging mainly due to t...

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Veröffentlicht in:	Analytical chemistry (Washington) 2017-05, Vol.89 (10), p.5467-5475
Hauptverfasser:	Cai, Wenxuan, Tucholski, Trisha, Chen, Bifan, Alpert, Andrew J, McIlwain, Sean, Kohmoto, Takushi, Jin, Song, Ge, Ying
Format:	Artikel
Sprache:	eng
Schlagworte:	Abundance Chemistry Chromatography Coupling (molecular) Decay Efficiency Fractionation Genetic diversity High resolution Internet Mass spectrometry Mass spectroscopy Molecular weight Noise prediction Post-translation Protein purification Proteins Proteomics Purification Robustness Separation Signal to noise ratio Size exclusion chromatography Strategy Translation
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