Immune responses induced by heat killed Saccharomyces cerevisiae : A vaccine against fungal infection
Abstract Heat-killed Saccharomyces cerevisiae (HKY) used as a vaccine protects mice against systemic aspergillosis and coccidioidomycosis. Little is known about the immune response induced by HKY vaccination, consequently our goal was to do an analysis of HKY-induced immune responses involved in pro...
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| creator | Liu, Min Clemons, Karl V Bigos, Marty Medovarska, Izabela Brummer, Elmer Stevens, David A |
| description | Abstract Heat-killed Saccharomyces cerevisiae (HKY) used as a vaccine protects mice against systemic aspergillosis and coccidioidomycosis. Little is known about the immune response induced by HKY vaccination, consequently our goal was to do an analysis of HKY-induced immune responses involved in protection. BALB/c mice were vaccinated subcutaneously 3 times with HKY, a protective reagent, and bronchoalveolar lavage fluid, spleen, lymph nodes, and serum collected 2–5 weeks later. Cultured spleen or lymph node cells were stimulated with HKY. Proliferation of HKY-stimulated spleen or lymph node cells was tested by Alamar Blue reduction and flow cytometry. Cytokines from lymphocyte supernatants and antibody to glycans in serum collected from HKY-vaccinated mice were measured by ELISA. The results show that HKY promoted spleen cell and lymph node cell proliferation from HKY-vaccinated mice but not from PBS-vaccinated control mice (all P < 0.05). Cytokine measurement showed HKY significantly promoted IFNγ, IL-6 and IL-17A production by spleen cells and lymph node cells (all P < 0.05 and P < 0.01, respectively). Cytokine production by HKY-stimulated cells from PBS-vaccinated mice was lower than those from HKY-vaccinated ( P < 0.05). Cytokines in BAL from HKY-vaccinated were higher, 1.7-fold for IFNγ and 2.1-fold for TNFα, than in BAL from PBS-vaccinated. Flow cytometry of lymphocytes from HKY-vaccinated showed 52% of CD3+ or 56% of CD8+ cells exhibited cell division after stimulation with HKY, compared to non-stimulated controls (26 or 23%, respectively) or HKY-stimulated cells from PBS-vaccinated (31 or 34%). HKY also induced antibody against Saccharomyces glucan and mannan with titers 4- or 2-fold, respectively, above that in unvaccinated. Taken together, the results suggested that HKY vaccination induces significant and specific Th1 type cellular immune responses and antibodies to glucan and mannan. |
| doi_str_mv | 10.1016/j.vaccine.2010.12.119 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5508752</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0264410X10019067</els_id><sourcerecordid>864961697</sourcerecordid><originalsourceid>FETCH-LOGICAL-c687t-ffcef647605b9ca4b58d3fae5b3387367f53c662e909a4cd9f468fe29e98abb53</originalsourceid><addsrcrecordid>eNqFkl9v0zAUxSMEYmXwEYBICPHU4j-xY_MwNE0DJk3ioUzizXKc69ZdYhc7qdRvP0ctG-xlT5btn889vucWxVuMFhhh_nmz2GljnIcFQdMZWWAsnxUzLGo6JwyL58UMEV7NK4x-nxSvUtoghBjF8mVxQjDBUtZ8VsBV348eyghpG3yCVDrfjgbastmXa9BDeeu6Lm-Xudpax9DvTYYMRNi55DSUX8rz8mil1CvtfBpKO_qV7rKUBTO44F8XL6zuErw5rqfFzbfLXxc_5tc_v19dnF_PDRf1MLfWgOVVzRFrpNFVw0RLrQbWUJq_xWvLqOGcgERSV6aVtuLCApEghW4aRk-Ls4Pudmx6aA34IepObaPrddyroJ36_8a7tVqFnWIMiZqRLPDpKBDDnxHSoHqXDHSd9hDGpASvJMdc1k-TDDGOhJjID4_ITRijz31QuJICY8QpzRQ7UCaGlCLYe9cYqSlxtVHHNqspcYWJyonnd-_-_fL9q78RZ-DjEdDJ6M5G7Y1LD1zuLCYMZe79gbM6KL2KmblZ5koMIUyFIFOprwcCcoQ7B1El48DnYXEx56za4J40e_ZIwXTOu2zrFvaQHvqiElFILacBnuYXZw8S8ZreAe1268E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1498110633</pqid></control><display><type>article</type><title>Immune responses induced by heat killed Saccharomyces cerevisiae : A vaccine against fungal infection</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><source>ProQuest Central UK/Ireland</source><creator>Liu, Min ; Clemons, Karl V ; Bigos, Marty ; Medovarska, Izabela ; Brummer, Elmer ; Stevens, David A</creator><creatorcontrib>Liu, Min ; Clemons, Karl V ; Bigos, Marty ; Medovarska, Izabela ; Brummer, Elmer ; Stevens, David A</creatorcontrib><description>Abstract Heat-killed Saccharomyces cerevisiae (HKY) used as a vaccine protects mice against systemic aspergillosis and coccidioidomycosis. Little is known about the immune response induced by HKY vaccination, consequently our goal was to do an analysis of HKY-induced immune responses involved in protection. BALB/c mice were vaccinated subcutaneously 3 times with HKY, a protective reagent, and bronchoalveolar lavage fluid, spleen, lymph nodes, and serum collected 2–5 weeks later. Cultured spleen or lymph node cells were stimulated with HKY. Proliferation of HKY-stimulated spleen or lymph node cells was tested by Alamar Blue reduction and flow cytometry. Cytokines from lymphocyte supernatants and antibody to glycans in serum collected from HKY-vaccinated mice were measured by ELISA. The results show that HKY promoted spleen cell and lymph node cell proliferation from HKY-vaccinated mice but not from PBS-vaccinated control mice (all P < 0.05). Cytokine measurement showed HKY significantly promoted IFNγ, IL-6 and IL-17A production by spleen cells and lymph node cells (all P < 0.05 and P < 0.01, respectively). Cytokine production by HKY-stimulated cells from PBS-vaccinated mice was lower than those from HKY-vaccinated ( P < 0.05). Cytokines in BAL from HKY-vaccinated were higher, 1.7-fold for IFNγ and 2.1-fold for TNFα, than in BAL from PBS-vaccinated. Flow cytometry of lymphocytes from HKY-vaccinated showed 52% of CD3+ or 56% of CD8+ cells exhibited cell division after stimulation with HKY, compared to non-stimulated controls (26 or 23%, respectively) or HKY-stimulated cells from PBS-vaccinated (31 or 34%). HKY also induced antibody against Saccharomyces glucan and mannan with titers 4- or 2-fold, respectively, above that in unvaccinated. Taken together, the results suggested that HKY vaccination induces significant and specific Th1 type cellular immune responses and antibodies to glucan and mannan.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2010.12.119</identifier><identifier>PMID: 21219976</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Algae ; Allergy and Immunology ; Animals ; antibodies ; Antigens, Fungal - immunology ; Applied microbiology ; aspergillosis ; Aspergillosis - immunology ; Aspergillosis - prevention & control ; Aspergillus ; Biological and medical sciences ; blood serum ; cell division ; Cell growth ; cell proliferation ; cell-mediated immunity ; Chitinase ; coccidioidomycosis ; Coccidioidomycosis - immunology ; Coccidioidomycosis - prevention & control ; Cytokines ; enzyme-linked immunosorbent assay ; Experiments ; flow cytometry ; Fundamental and applied biological sciences. Psychology ; Fungal infection ; Fungal infections ; Fungal Vaccines - administration & dosage ; Fungal Vaccines - immunology ; fungi ; Gene expression ; glucans ; heat ; Hot Temperature ; Immune response ; Immune system ; interleukin-17 ; interleukin-6 ; Lymph nodes ; Lymph Nodes - cytology ; Lymph Nodes - immunology ; Lymph Nodes - microbiology ; Lymphocytes ; Male ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Mycology ; Proteins ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - immunology ; Spleen ; Spleen - cytology ; Spleen - immunology ; Spleen - microbiology ; splenocytes ; Studies ; tumor necrosis factor-alpha ; vaccination ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, Inactivated - administration & dosage ; Vaccines, Inactivated - immunology</subject><ispartof>Vaccine, 2011-02, Vol.29 (9), p.1745-1753</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Feb 17, 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c687t-ffcef647605b9ca4b58d3fae5b3387367f53c662e909a4cd9f468fe29e98abb53</citedby><cites>FETCH-LOGICAL-c687t-ffcef647605b9ca4b58d3fae5b3387367f53c662e909a4cd9f468fe29e98abb53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1498110633?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27929,27930,46000,64390,64392,64394,72474</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23871250$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21219976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Min</creatorcontrib><creatorcontrib>Clemons, Karl V</creatorcontrib><creatorcontrib>Bigos, Marty</creatorcontrib><creatorcontrib>Medovarska, Izabela</creatorcontrib><creatorcontrib>Brummer, Elmer</creatorcontrib><creatorcontrib>Stevens, David A</creatorcontrib><title>Immune responses induced by heat killed Saccharomyces cerevisiae : A vaccine against fungal infection</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Heat-killed Saccharomyces cerevisiae (HKY) used as a vaccine protects mice against systemic aspergillosis and coccidioidomycosis. Little is known about the immune response induced by HKY vaccination, consequently our goal was to do an analysis of HKY-induced immune responses involved in protection. BALB/c mice were vaccinated subcutaneously 3 times with HKY, a protective reagent, and bronchoalveolar lavage fluid, spleen, lymph nodes, and serum collected 2–5 weeks later. Cultured spleen or lymph node cells were stimulated with HKY. Proliferation of HKY-stimulated spleen or lymph node cells was tested by Alamar Blue reduction and flow cytometry. Cytokines from lymphocyte supernatants and antibody to glycans in serum collected from HKY-vaccinated mice were measured by ELISA. The results show that HKY promoted spleen cell and lymph node cell proliferation from HKY-vaccinated mice but not from PBS-vaccinated control mice (all P < 0.05). Cytokine measurement showed HKY significantly promoted IFNγ, IL-6 and IL-17A production by spleen cells and lymph node cells (all P < 0.05 and P < 0.01, respectively). Cytokine production by HKY-stimulated cells from PBS-vaccinated mice was lower than those from HKY-vaccinated ( P < 0.05). Cytokines in BAL from HKY-vaccinated were higher, 1.7-fold for IFNγ and 2.1-fold for TNFα, than in BAL from PBS-vaccinated. Flow cytometry of lymphocytes from HKY-vaccinated showed 52% of CD3+ or 56% of CD8+ cells exhibited cell division after stimulation with HKY, compared to non-stimulated controls (26 or 23%, respectively) or HKY-stimulated cells from PBS-vaccinated (31 or 34%). HKY also induced antibody against Saccharomyces glucan and mannan with titers 4- or 2-fold, respectively, above that in unvaccinated. Taken together, the results suggested that HKY vaccination induces significant and specific Th1 type cellular immune responses and antibodies to glucan and mannan.</description><subject>Algae</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>antibodies</subject><subject>Antigens, Fungal - immunology</subject><subject>Applied microbiology</subject><subject>aspergillosis</subject><subject>Aspergillosis - immunology</subject><subject>Aspergillosis - prevention & control</subject><subject>Aspergillus</subject><subject>Biological and medical sciences</subject><subject>blood serum</subject><subject>cell division</subject><subject>Cell growth</subject><subject>cell proliferation</subject><subject>cell-mediated immunity</subject><subject>Chitinase</subject><subject>coccidioidomycosis</subject><subject>Coccidioidomycosis - immunology</subject><subject>Coccidioidomycosis - prevention & control</subject><subject>Cytokines</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Experiments</subject><subject>flow cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal infection</subject><subject>Fungal infections</subject><subject>Fungal Vaccines - administration & dosage</subject><subject>Fungal Vaccines - immunology</subject><subject>fungi</subject><subject>Gene expression</subject><subject>glucans</subject><subject>heat</subject><subject>Hot Temperature</subject><subject>Immune response</subject><subject>Immune system</subject><subject>interleukin-17</subject><subject>interleukin-6</subject><subject>Lymph nodes</subject><subject>Lymph Nodes - cytology</subject><subject>Lymph Nodes - immunology</subject><subject>Lymph Nodes - microbiology</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Proteins</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - immunology</subject><subject>Spleen</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Spleen - microbiology</subject><subject>splenocytes</subject><subject>Studies</subject><subject>tumor necrosis factor-alpha</subject><subject>vaccination</subject><subject>Vaccines</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccines, Inactivated - administration & dosage</subject><subject>Vaccines, Inactivated - immunology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkl9v0zAUxSMEYmXwEYBICPHU4j-xY_MwNE0DJk3ioUzizXKc69ZdYhc7qdRvP0ctG-xlT5btn889vucWxVuMFhhh_nmz2GljnIcFQdMZWWAsnxUzLGo6JwyL58UMEV7NK4x-nxSvUtoghBjF8mVxQjDBUtZ8VsBV348eyghpG3yCVDrfjgbastmXa9BDeeu6Lm-Xudpax9DvTYYMRNi55DSUX8rz8mil1CvtfBpKO_qV7rKUBTO44F8XL6zuErw5rqfFzbfLXxc_5tc_v19dnF_PDRf1MLfWgOVVzRFrpNFVw0RLrQbWUJq_xWvLqOGcgERSV6aVtuLCApEghW4aRk-Ls4Pudmx6aA34IepObaPrddyroJ36_8a7tVqFnWIMiZqRLPDpKBDDnxHSoHqXDHSd9hDGpASvJMdc1k-TDDGOhJjID4_ITRijz31QuJICY8QpzRQ7UCaGlCLYe9cYqSlxtVHHNqspcYWJyonnd-_-_fL9q78RZ-DjEdDJ6M5G7Y1LD1zuLCYMZe79gbM6KL2KmblZ5koMIUyFIFOprwcCcoQ7B1El48DnYXEx56za4J40e_ZIwXTOu2zrFvaQHvqiElFILacBnuYXZw8S8ZreAe1268E</recordid><startdate>20110217</startdate><enddate>20110217</enddate><creator>Liu, Min</creator><creator>Clemons, Karl V</creator><creator>Bigos, Marty</creator><creator>Medovarska, Izabela</creator><creator>Brummer, Elmer</creator><creator>Stevens, David A</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110217</creationdate><title>Immune responses induced by heat killed Saccharomyces cerevisiae : A vaccine against fungal infection</title><author>Liu, Min ; Clemons, Karl V ; Bigos, Marty ; Medovarska, Izabela ; Brummer, Elmer ; Stevens, David A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c687t-ffcef647605b9ca4b58d3fae5b3387367f53c662e909a4cd9f468fe29e98abb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Algae</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>antibodies</topic><topic>Antigens, Fungal - immunology</topic><topic>Applied microbiology</topic><topic>aspergillosis</topic><topic>Aspergillosis - immunology</topic><topic>Aspergillosis - prevention & control</topic><topic>Aspergillus</topic><topic>Biological and medical sciences</topic><topic>blood serum</topic><topic>cell division</topic><topic>Cell growth</topic><topic>cell proliferation</topic><topic>cell-mediated immunity</topic><topic>Chitinase</topic><topic>coccidioidomycosis</topic><topic>Coccidioidomycosis - immunology</topic><topic>Coccidioidomycosis - prevention & control</topic><topic>Cytokines</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Experiments</topic><topic>flow cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal infection</topic><topic>Fungal infections</topic><topic>Fungal Vaccines - administration & dosage</topic><topic>Fungal Vaccines - immunology</topic><topic>fungi</topic><topic>Gene expression</topic><topic>glucans</topic><topic>heat</topic><topic>Hot Temperature</topic><topic>Immune response</topic><topic>Immune system</topic><topic>interleukin-17</topic><topic>interleukin-6</topic><topic>Lymph nodes</topic><topic>Lymph Nodes - cytology</topic><topic>Lymph Nodes - immunology</topic><topic>Lymph Nodes - microbiology</topic><topic>Lymphocytes</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Proteins</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - immunology</topic><topic>Spleen</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Spleen - microbiology</topic><topic>splenocytes</topic><topic>Studies</topic><topic>tumor necrosis factor-alpha</topic><topic>vaccination</topic><topic>Vaccines</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccines, Inactivated - administration & dosage</topic><topic>Vaccines, Inactivated - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Min</creatorcontrib><creatorcontrib>Clemons, Karl V</creatorcontrib><creatorcontrib>Bigos, Marty</creatorcontrib><creatorcontrib>Medovarska, Izabela</creatorcontrib><creatorcontrib>Brummer, Elmer</creatorcontrib><creatorcontrib>Stevens, David A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Min</au><au>Clemons, Karl V</au><au>Bigos, Marty</au><au>Medovarska, Izabela</au><au>Brummer, Elmer</au><au>Stevens, David A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune responses induced by heat killed Saccharomyces cerevisiae : A vaccine against fungal infection</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2011-02-17</date><risdate>2011</risdate><volume>29</volume><issue>9</issue><spage>1745</spage><epage>1753</epage><pages>1745-1753</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Abstract Heat-killed Saccharomyces cerevisiae (HKY) used as a vaccine protects mice against systemic aspergillosis and coccidioidomycosis. Little is known about the immune response induced by HKY vaccination, consequently our goal was to do an analysis of HKY-induced immune responses involved in protection. BALB/c mice were vaccinated subcutaneously 3 times with HKY, a protective reagent, and bronchoalveolar lavage fluid, spleen, lymph nodes, and serum collected 2–5 weeks later. Cultured spleen or lymph node cells were stimulated with HKY. Proliferation of HKY-stimulated spleen or lymph node cells was tested by Alamar Blue reduction and flow cytometry. Cytokines from lymphocyte supernatants and antibody to glycans in serum collected from HKY-vaccinated mice were measured by ELISA. The results show that HKY promoted spleen cell and lymph node cell proliferation from HKY-vaccinated mice but not from PBS-vaccinated control mice (all P < 0.05). Cytokine measurement showed HKY significantly promoted IFNγ, IL-6 and IL-17A production by spleen cells and lymph node cells (all P < 0.05 and P < 0.01, respectively). Cytokine production by HKY-stimulated cells from PBS-vaccinated mice was lower than those from HKY-vaccinated ( P < 0.05). Cytokines in BAL from HKY-vaccinated were higher, 1.7-fold for IFNγ and 2.1-fold for TNFα, than in BAL from PBS-vaccinated. Flow cytometry of lymphocytes from HKY-vaccinated showed 52% of CD3+ or 56% of CD8+ cells exhibited cell division after stimulation with HKY, compared to non-stimulated controls (26 or 23%, respectively) or HKY-stimulated cells from PBS-vaccinated (31 or 34%). HKY also induced antibody against Saccharomyces glucan and mannan with titers 4- or 2-fold, respectively, above that in unvaccinated. Taken together, the results suggested that HKY vaccination induces significant and specific Th1 type cellular immune responses and antibodies to glucan and mannan.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21219976</pmid><doi>10.1016/j.vaccine.2010.12.119</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5508752 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); ProQuest Central UK/Ireland |
| subjects | Algae Allergy and Immunology Animals antibodies Antigens, Fungal - immunology Applied microbiology aspergillosis Aspergillosis - immunology Aspergillosis - prevention & control Aspergillus Biological and medical sciences blood serum cell division Cell growth cell proliferation cell-mediated immunity Chitinase coccidioidomycosis Coccidioidomycosis - immunology Coccidioidomycosis - prevention & control Cytokines enzyme-linked immunosorbent assay Experiments flow cytometry Fundamental and applied biological sciences. Psychology Fungal infection Fungal infections Fungal Vaccines - administration & dosage Fungal Vaccines - immunology fungi Gene expression glucans heat Hot Temperature Immune response Immune system interleukin-17 interleukin-6 Lymph nodes Lymph Nodes - cytology Lymph Nodes - immunology Lymph Nodes - microbiology Lymphocytes Male Mice Mice, Inbred BALB C Microbiology Miscellaneous Mycology Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - immunology Spleen Spleen - cytology Spleen - immunology Spleen - microbiology splenocytes Studies tumor necrosis factor-alpha vaccination Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Inactivated - administration & dosage Vaccines, Inactivated - immunology |
| title | Immune responses induced by heat killed Saccharomyces cerevisiae : A vaccine against fungal infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T02%3A05%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immune%20responses%20induced%20by%20heat%20killed%20Saccharomyces%20cerevisiae%20:%20A%20vaccine%20against%20fungal%20infection&rft.jtitle=Vaccine&rft.au=Liu,%20Min&rft.date=2011-02-17&rft.volume=29&rft.issue=9&rft.spage=1745&rft.epage=1753&rft.pages=1745-1753&rft.issn=0264-410X&rft.eissn=1873-2518&rft.coden=VACCDE&rft_id=info:doi/10.1016/j.vaccine.2010.12.119&rft_dat=%3Cproquest_pubme%3E864961697%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1498110633&rft_id=info:pmid/21219976&rft_els_id=S0264410X10019067&rfr_iscdi=true |