Improving target amino acid selectivity in a permissive aminoacyl tRNA synthetase through counter-selection

The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein dynamics, either alone or as part of a Förster resonance energy transfer (FRET) or photo-induced electron transfer (eT) probe pair. We have previously reported the genetic incorporation of Acd by an aminoacyl tRNA synthetase...

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Veröffentlicht in:	Organic & biomolecular chemistry 2017-05, Vol.15 (17), p.3603-3610
Hauptverfasser:	Sungwienwong, Itthipol, Hostetler, Zachary M, Blizzard, Robert J, Porter, Joseph J, Driggers, Camden M, Mbengi, Lea Z, Villegas, José A, Speight, Lee C, Saven, Jeffery G, Perona, John J, Kohli, Rahul M, Mehl, Ryan A, Petersson, E James
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - metabolism Amino Acyl-tRNA Synthetases - chemistry Amino Acyl-tRNA Synthetases - metabolism Catalytic Domain Fluorescence Resonance Energy Transfer Kinetics Models, Molecular Protein Binding
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