Preparation, Crystallization, and Structure Determination of Chromatin Enzyme/Nucleosome Complexes

Structural studies of chromatin complexes composed of chromatin factors or enzymes bound to the nucleosome have been constrained by the ability to produce high-quality complexes in the amounts appropriate for biophysical studies and by the difficulty of crystallizing these complexes. We describe her...

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Veröffentlicht in:	Methods in enzymology 2016, Vol.573, p.43-65
Hauptverfasser:	McGinty, R K, Makde, R D, Tan, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatin - chemistry Chromatography, Gel - methods Crystallization - methods DNA - chemistry Fluorescent Dyes - chemistry Humans Nucleosomes - chemistry
Online-Zugang:	Volltext
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