Virus-induced inflammasome activation is suppressed by prostaglandin D₂/DP1 signaling

Prostaglandin D2 (PGD₂), an eicosanoid with both pro- and antiinflammatory properties, is the most abundantly expressed prostaglandin in the brain. Here we show that PGD₂ signaling through the D-prostanoid receptor 1 (DP1) receptor is necessary for optimal microglia/macrophage activation and IFN exp...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2017-07, Vol.114 (27), p.E5444-E5453
Hauptverfasser: Vijay, Rahul, Fehr, Anthony R., Janowski, Ann M., Athmer, Jeremiah, Wheeler, Dorthea L., Grunewald, Matthew, Sompallae, Ramakrishna, Kurup, Samarchith P., Meyerholz, David K., Sutterwala, Fayyaz S., Narumiya, Shuh, Perlman, Stanley
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container_end_page E5453
container_issue 27
container_start_page E5444
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 114
creator Vijay, Rahul
Fehr, Anthony R.
Janowski, Ann M.
Athmer, Jeremiah
Wheeler, Dorthea L.
Grunewald, Matthew
Sompallae, Ramakrishna
Kurup, Samarchith P.
Meyerholz, David K.
Sutterwala, Fayyaz S.
Narumiya, Shuh
Perlman, Stanley
description Prostaglandin D2 (PGD₂), an eicosanoid with both pro- and antiinflammatory properties, is the most abundantly expressed prostaglandin in the brain. Here we show that PGD₂ signaling through the D-prostanoid receptor 1 (DP1) receptor is necessary for optimal microglia/macrophage activation and IFN expression after infection with a neurotropic coronavirus. Genome-wide expression analyses indicated that PGD₂/DP1 signaling is required for up-regulation of a putative inflammasome inhibitor, PYDC3, in CD11b⁺ cells in the CNS of infected mice. Our results also demonstrated that, in addition to PGD₂/DP1 signaling, type 1 IFN (IFN-I) signaling is required for PYDC3 expression. In the absence of Pydc3 up-regulation, IL-1β expression and, subsequently, mortality were increased in infected DP1 −/− mice. Notably, survival was enhanced by IL1 receptor blockade, indicating that the effects of the absence of DP1 signaling on clinical outcomes were mediated, at least in part, by inflammasomes. Using bone marrow-derived macrophages in vitro, we confirmed that PYDC3 expression is dependent upon DP1 signaling and that IFN priming is critical for PYDC3 up-regulation. In addition, Pydc3 silencing or overexpression augmented or diminished IL-1β secretion, respectively. Furthermore, DP1 signaling in human macrophages also resulted in the up-regulation of a putative functional analog, POP3, suggesting that PGD₂ similarly modulates inflammasomes in human cells. These findings demonstrate a previously undescribed role for prostaglandin signaling in preventing excessive inflammasome activation and, together with previously published results, suggest that eicosanoids and inflammasomes are reciprocally regulated.
doi_str_mv 10.1073/pnas.1704099114
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PNAS Plus
title Virus-induced inflammasome activation is suppressed by prostaglandin D₂/DP1 signaling
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