Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system
An increase in Lewis- and core-type fucosylation of haptoglobin has been reported in patients with pancreatic cancer (PC), suggesting that fucosylated haptoglobin is a candidate PC biomarker. Previously, we developed a Pholiota squarrosa lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA...
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| Veröffentlicht in: | Glycoconjugate journal 2017-08, Vol.34 (4), p.537-544 |
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| container_title | Glycoconjugate journal |
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| creator | Kusama, Ken Okamoto, Yuki Saito, Keiko Kasahara, Tsukasa Murata, Teizo Ueno, Yasushi Kobayashi, Yuka Kamada, Yoshihiro Miyoshi, Eiji |
| description | An increase in Lewis- and core-type fucosylation of haptoglobin has been reported in patients with pancreatic cancer (PC), suggesting that fucosylated haptoglobin is a candidate PC biomarker. Previously, we developed a
Pholiota squarrosa
lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection of core-fucosylated haptoglobin. However, with this methodology, positive results were only obtained for some patients with PC, demonstrating the need for a more sensitive detection system. In the current study, we developed an improved PhoSL-ELISA system with higher sensitivity to detect core-fucosylated haptoglobin using high-concentration urea as a denaturing agent with lectin to facilitate detection. We then reevaluated the performance of PhoSL reactive-core-fucosylated haptoglobin (PhoSL-HP) as a PC biomarker using the improved PhoSL-ELISA system. PhoSL-HP levels in the sera of patients with PC were significantly higher than those in healthy volunteers, with an area under the curve (AUC) value of 0.753. Furthermore, the AUC value of CA19–9 improved from 0.793 to 0.907 when combined with PhoSL-HP. Additionally, several CA19–9-negative cases among the patients with PC were diagnosed as positive for PhoSL-HP. In conclusion, PhoSL-HP detection using our improved ELISA system might allow PhoSL-HP to serve as a potential biomarker for PC and thus might be useful to complement the detection of CA19–9 in PC diagnosis. |
| doi_str_mv | 10.1007/s10719-017-9772-9 |
| format | Article |
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Pholiota squarrosa
lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection of core-fucosylated haptoglobin. However, with this methodology, positive results were only obtained for some patients with PC, demonstrating the need for a more sensitive detection system. In the current study, we developed an improved PhoSL-ELISA system with higher sensitivity to detect core-fucosylated haptoglobin using high-concentration urea as a denaturing agent with lectin to facilitate detection. We then reevaluated the performance of PhoSL reactive-core-fucosylated haptoglobin (PhoSL-HP) as a PC biomarker using the improved PhoSL-ELISA system. PhoSL-HP levels in the sera of patients with PC were significantly higher than those in healthy volunteers, with an area under the curve (AUC) value of 0.753. Furthermore, the AUC value of CA19–9 improved from 0.793 to 0.907 when combined with PhoSL-HP. Additionally, several CA19–9-negative cases among the patients with PC were diagnosed as positive for PhoSL-HP. In conclusion, PhoSL-HP detection using our improved ELISA system might allow PhoSL-HP to serve as a potential biomarker for PC and thus might be useful to complement the detection of CA19–9 in PC diagnosis.</description><identifier>ISSN: 0282-0080</identifier><identifier>EISSN: 1573-4986</identifier><identifier>DOI: 10.1007/s10719-017-9772-9</identifier><identifier>PMID: 28455724</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adult ; Aged ; Antigens, Tumor-Associated, Carbohydrate - metabolism ; Assaying ; Biochemistry ; Biomarkers ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - metabolism ; Biomedical and Life Sciences ; Biotinylation ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Haptoglobin ; Haptoglobins - metabolism ; Hep G2 Cells ; Humans ; Lectins ; Lectins - metabolism ; Life Sciences ; Male ; Middle Aged ; Original ; Original Article ; Pancreatic cancer ; Pancreatic Neoplasms - blood ; Pancreatic Neoplasms - metabolism ; Pathology ; Pholiota - metabolism ; Protein Denaturation ; Urea</subject><ispartof>Glycoconjugate journal, 2017-08, Vol.34 (4), p.537-544</ispartof><rights>The Author(s) 2017</rights><rights>Glycoconjugate Journal is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-d100b56f21e21da9a7ea418ad2ec4d6446956b1d7c2cb658f2fb355806fb8c363</citedby><cites>FETCH-LOGICAL-c536t-d100b56f21e21da9a7ea418ad2ec4d6446956b1d7c2cb658f2fb355806fb8c363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10719-017-9772-9$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10719-017-9772-9$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28455724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kusama, Ken</creatorcontrib><creatorcontrib>Okamoto, Yuki</creatorcontrib><creatorcontrib>Saito, Keiko</creatorcontrib><creatorcontrib>Kasahara, Tsukasa</creatorcontrib><creatorcontrib>Murata, Teizo</creatorcontrib><creatorcontrib>Ueno, Yasushi</creatorcontrib><creatorcontrib>Kobayashi, Yuka</creatorcontrib><creatorcontrib>Kamada, Yoshihiro</creatorcontrib><creatorcontrib>Miyoshi, Eiji</creatorcontrib><title>Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system</title><title>Glycoconjugate journal</title><addtitle>Glycoconj J</addtitle><addtitle>Glycoconj J</addtitle><description>An increase in Lewis- and core-type fucosylation of haptoglobin has been reported in patients with pancreatic cancer (PC), suggesting that fucosylated haptoglobin is a candidate PC biomarker. Previously, we developed a
Pholiota squarrosa
lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection of core-fucosylated haptoglobin. However, with this methodology, positive results were only obtained for some patients with PC, demonstrating the need for a more sensitive detection system. In the current study, we developed an improved PhoSL-ELISA system with higher sensitivity to detect core-fucosylated haptoglobin using high-concentration urea as a denaturing agent with lectin to facilitate detection. We then reevaluated the performance of PhoSL reactive-core-fucosylated haptoglobin (PhoSL-HP) as a PC biomarker using the improved PhoSL-ELISA system. PhoSL-HP levels in the sera of patients with PC were significantly higher than those in healthy volunteers, with an area under the curve (AUC) value of 0.753. Furthermore, the AUC value of CA19–9 improved from 0.793 to 0.907 when combined with PhoSL-HP. Additionally, several CA19–9-negative cases among the patients with PC were diagnosed as positive for PhoSL-HP. In conclusion, PhoSL-HP detection using our improved ELISA system might allow PhoSL-HP to serve as a potential biomarker for PC and thus might be useful to complement the detection of CA19–9 in PC diagnosis.</description><subject>Adult</subject><subject>Aged</subject><subject>Antigens, Tumor-Associated, Carbohydrate - metabolism</subject><subject>Assaying</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biotinylation</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Haptoglobin</subject><subject>Haptoglobins - metabolism</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Lectins</subject><subject>Lectins - metabolism</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original</subject><subject>Original Article</subject><subject>Pancreatic cancer</subject><subject>Pancreatic Neoplasms - blood</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Pathology</subject><subject>Pholiota - metabolism</subject><subject>Protein Denaturation</subject><subject>Urea</subject><issn>0282-0080</issn><issn>1573-4986</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1UU1rFTEUDaLY1-oPcCMBN25Gk8zkayOUUtvCA6XVdbiTybyXOpNMk5kH3fjbm8erpQqu7oXzkXtyEHpHySdKiPycKZFUV4TKSkvJKv0CrSiXddVoJV6iFWGKVYQocoSOc74lRdMw9RodMdVwLlmzQr-vndvBsMDsY8Cxx9-3cfBxBpzvFkgpZsCDs7MPVXJQ5s7hLUxz3Ayx9QFDxoAnCLags7fYltUl3Po4QvpVtiX7sMEQsB-nFHeuw-frq5tTnO_z7MY36FUPQ3ZvH-cJ-vn1_MfZZbX-dnF1drquLK_FXHUlbstFz6hjtAMN0kFDFXTM2aYTTSM0Fy3tpGW2FVz1rG9rzhURfatsLeoT9OXgOy3t6DrrwpxgMFPy5cx7E8Gbv5Hgt2YTd4ZzwghXxeDjo0GKd4vLsxl9tm4YILi4ZEOVrnmjhdhTP_xDvY1LCiWeoZpKShVXexY9sGz545xc_3QMJWbfrjm0a0q7Zt-u0UXz_nmKJ8WfOguBHQi5QGHj0rOn_-v6AEUKsss</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Kusama, Ken</creator><creator>Okamoto, Yuki</creator><creator>Saito, Keiko</creator><creator>Kasahara, Tsukasa</creator><creator>Murata, Teizo</creator><creator>Ueno, Yasushi</creator><creator>Kobayashi, Yuka</creator><creator>Kamada, Yoshihiro</creator><creator>Miyoshi, Eiji</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170801</creationdate><title>Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system</title><author>Kusama, Ken ; Okamoto, Yuki ; Saito, Keiko ; Kasahara, Tsukasa ; Murata, Teizo ; Ueno, Yasushi ; Kobayashi, Yuka ; Kamada, Yoshihiro ; Miyoshi, Eiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-d100b56f21e21da9a7ea418ad2ec4d6446956b1d7c2cb658f2fb355806fb8c363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antigens, Tumor-Associated, Carbohydrate - metabolism</topic><topic>Assaying</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - blood</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biotinylation</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Haptoglobin</topic><topic>Haptoglobins - metabolism</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Lectins</topic><topic>Lectins - metabolism</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original</topic><topic>Original Article</topic><topic>Pancreatic cancer</topic><topic>Pancreatic Neoplasms - blood</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Pathology</topic><topic>Pholiota - metabolism</topic><topic>Protein Denaturation</topic><topic>Urea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kusama, Ken</creatorcontrib><creatorcontrib>Okamoto, Yuki</creatorcontrib><creatorcontrib>Saito, Keiko</creatorcontrib><creatorcontrib>Kasahara, Tsukasa</creatorcontrib><creatorcontrib>Murata, Teizo</creatorcontrib><creatorcontrib>Ueno, Yasushi</creatorcontrib><creatorcontrib>Kobayashi, Yuka</creatorcontrib><creatorcontrib>Kamada, Yoshihiro</creatorcontrib><creatorcontrib>Miyoshi, Eiji</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Glycoconjugate journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kusama, Ken</au><au>Okamoto, Yuki</au><au>Saito, Keiko</au><au>Kasahara, Tsukasa</au><au>Murata, Teizo</au><au>Ueno, Yasushi</au><au>Kobayashi, Yuka</au><au>Kamada, Yoshihiro</au><au>Miyoshi, Eiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system</atitle><jtitle>Glycoconjugate journal</jtitle><stitle>Glycoconj J</stitle><addtitle>Glycoconj J</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>34</volume><issue>4</issue><spage>537</spage><epage>544</epage><pages>537-544</pages><issn>0282-0080</issn><eissn>1573-4986</eissn><abstract>An increase in Lewis- and core-type fucosylation of haptoglobin has been reported in patients with pancreatic cancer (PC), suggesting that fucosylated haptoglobin is a candidate PC biomarker. Previously, we developed a
Pholiota squarrosa
lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection of core-fucosylated haptoglobin. However, with this methodology, positive results were only obtained for some patients with PC, demonstrating the need for a more sensitive detection system. In the current study, we developed an improved PhoSL-ELISA system with higher sensitivity to detect core-fucosylated haptoglobin using high-concentration urea as a denaturing agent with lectin to facilitate detection. We then reevaluated the performance of PhoSL reactive-core-fucosylated haptoglobin (PhoSL-HP) as a PC biomarker using the improved PhoSL-ELISA system. PhoSL-HP levels in the sera of patients with PC were significantly higher than those in healthy volunteers, with an area under the curve (AUC) value of 0.753. Furthermore, the AUC value of CA19–9 improved from 0.793 to 0.907 when combined with PhoSL-HP. Additionally, several CA19–9-negative cases among the patients with PC were diagnosed as positive for PhoSL-HP. In conclusion, PhoSL-HP detection using our improved ELISA system might allow PhoSL-HP to serve as a potential biomarker for PC and thus might be useful to complement the detection of CA19–9 in PC diagnosis.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28455724</pmid><doi>10.1007/s10719-017-9772-9</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Antigens, Tumor-Associated, Carbohydrate - metabolism Assaying Biochemistry Biomarkers Biomarkers, Tumor - blood Biomarkers, Tumor - metabolism Biomedical and Life Sciences Biotinylation Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Female Haptoglobin Haptoglobins - metabolism Hep G2 Cells Humans Lectins Lectins - metabolism Life Sciences Male Middle Aged Original Original Article Pancreatic cancer Pancreatic Neoplasms - blood Pancreatic Neoplasms - metabolism Pathology Pholiota - metabolism Protein Denaturation Urea |
| title | Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system |
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