A Triple-Fluorophore-Labeled Nucleic Acid pH Nanosensor to Investigate Non-viral Gene Delivery

There is a need for new tools to better quantify intracellular delivery barriers in high-throughput and high-content ways. Here, we synthesized a triple-fluorophore-labeled nucleic acid pH nanosensor for measuring intracellular pH of exogenous DNA at specific time points in a high-throughput manner...

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Veröffentlicht in:	Molecular therapy 2017-07, Vol.25 (7), p.1697-1709
Hauptverfasser:	Wilson, David R., Routkevitch, Denis, Rui, Yuan, Mosenia, Arman, Wahlin, Karl J., Quinones-Hinojosa, Alfredo, Zack, Donald J., Green, Jordan J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acidification Animals Biosensing Techniques - methods Carbocyanines - chemistry Carboxylic Acids - chemistry Deoxyribonucleic acid DNA DNA - chemistry DNA - genetics DNA - metabolism Flow cytometry Flow Cytometry - methods Fluorescein - chemistry Fluorescent Dyes - chemistry Fluorophores gene delivery Gene Expression Gene transfer Gene Transfer Techniques Genes, Reporter Glycerol Humans Hydrogen-Ion Concentration Intracellular Investigations Luminescent Proteins - genetics Luminescent Proteins - metabolism Lysine Microscopy nanoparticle Nanoparticles Nanoparticles - chemistry non-viral Original Particle Size pH effects pH sensor Physiology Plasmids Poly-L-lysine Polyethyleneimine - chemistry Polylysine - chemistry polymer Polymers proton sponge Reporter gene Scholarships & fellowships Single-Cell Analysis - methods Staining and Labeling - methods Transfection
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