Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)
Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has yet to win broad acceptance in rout...
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| Veröffentlicht in: | Biomolecular detection and quantification 2017-06, Vol.12, p.1-6 |
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| creator | Forootan, Amin Sjöback, Robert Björkman, Jens Sjögreen, Björn Linz, Lucas Kubista, Mikael |
| description | Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has yet to win broad acceptance in routine practice. This requires a means to unambiguously assess the performance of specific qPCR analyses. Here we present methods to determine the limit of detection (LoD) and the limit of quantification (LoQ) as applicable to qPCR. These are based on standard statistical methods as recommended by regulatory bodies adapted to qPCR and complemented with a novel approach to estimate the precision of LoD. |
| doi_str_mv | 10.1016/j.bdq.2017.04.001 |
| format | Article |
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| subjects | Data analysis GenEx software Limit of detection Limit of quantification LoD LoQ MIQE qPCR Quality control Real-time PCR Replicates Research Paper Standardization |
| title | Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR) |
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