Characterization of aminoacyl-tRNA synthetase stability and substrate interaction by differential scanning fluorimetry

•AARS protein stability can be evaluated by differential scanning fluorimetry (DSF).•DSF can be used for determining the stability of AARS enzyme substitutions.•AARS amino acid and inhibitor binding studies can be conducted with DSF.•Thermal stability can change as a function of tRNA binding and be...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2017-01, Vol.113, p.64-71
Hauptverfasser:	Abbott, Jamie A., Livingston, Nathan M., Egri, Shawn B., Guth, Ethan, Francklyn, Christopher S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine-tRNA Ligase - antagonists & inhibitors Alanine-tRNA Ligase - chemistry Alanine-tRNA Ligase - genetics Alanine-tRNA Ligase - metabolism Amino acid Amino Acids - chemistry Amino Acids - metabolism Aminoacyl-tRNA synthetase Benzopyrans - chemistry Differential scanning fluorimetry Enzyme Inhibitors - chemistry Enzyme Stability Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Proteins - antagonists & inhibitors Escherichia coli Proteins - chemistry Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fluorescent Dyes - chemistry Fluorometry - methods Histidine-tRNA Ligase - antagonists & inhibitors Histidine-tRNA Ligase - chemistry Histidine-tRNA Ligase - genetics Histidine-tRNA Ligase - metabolism Histidyl-tRNA synthetase Muramidase - chemistry Muramidase - metabolism Phase Transition Protein Binding Protein Unfolding RNA, Transfer, Amino Acid-Specific - genetics RNA, Transfer, Amino Acid-Specific - metabolism Substrate Specificity SYPRO Orange Threonine-tRNA Ligase - antagonists & inhibitors Threonine-tRNA Ligase - chemistry Threonine-tRNA Ligase - genetics Threonine-tRNA Ligase - metabolism Transfer RNA Aminoacylation tRNA
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!