Differential microRNA expression profiling in primary tumors and matched liver metastasis of patients with colorectal cancer
Liver metastasis is common in patients with colorectal cancer (CRC), and is correlated with poor outcome. MicroRNAs (miRNAs) are small non-coding RNAs involved in cancer development and progression, but their role in CRC liver metastasis has not been extensively investigated. Thirteen miRNAs were de...
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Veröffentlicht in: | Oncotarget 2017-05, Vol.8 (22), p.35783-35791 |
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creator | Li, Wenhua Chang, Jinjia Tong, Duo Peng, Junjie Huang, Dan Guo, Weijian Zhang, Wen Li, Jin |
description | Liver metastasis is common in patients with colorectal cancer (CRC), and is correlated with poor outcome. MicroRNAs (miRNAs) are small non-coding RNAs involved in cancer development and progression, but their role in CRC liver metastasis has not been extensively investigated.
Thirteen miRNAs were deregulated in pCRCs compared to their matched liver metastases. Seventeen miRNAs were chosen for validation, which confirmed significantly reduced expression of miR-99b-5p, miR-377 and miR-200c and increased expression of miR-196b-5p in the tissue of liver metastasis. Furthermore, miR-200c and miR-196b-5p were positively correlated with shorter overall survival in pCRC patients with liver metastasis.
Firstly, affymetrix microarrays involving 1036 miRNAs were performed in two pairs of primary CRCs (pCRCs) and their matched liver metastases. Secondly, validation of the results was carried out on an independent cohort of 48 pairs of pCRCs and matched liver metastases using quantitative real-time polymerase chain reaction assay.
We discovered a pCRC liver metastasis-specific miRNA panel including miR-377, miR-99b-5p, miR-200c and miR-196b-5p through intensive validation. These miRNAs may function as prognostic factors in patients with metastatic CRC. |
doi_str_mv | 10.18632/oncotarget.16206 |
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Thirteen miRNAs were deregulated in pCRCs compared to their matched liver metastases. Seventeen miRNAs were chosen for validation, which confirmed significantly reduced expression of miR-99b-5p, miR-377 and miR-200c and increased expression of miR-196b-5p in the tissue of liver metastasis. Furthermore, miR-200c and miR-196b-5p were positively correlated with shorter overall survival in pCRC patients with liver metastasis.
Firstly, affymetrix microarrays involving 1036 miRNAs were performed in two pairs of primary CRCs (pCRCs) and their matched liver metastases. Secondly, validation of the results was carried out on an independent cohort of 48 pairs of pCRCs and matched liver metastases using quantitative real-time polymerase chain reaction assay.
We discovered a pCRC liver metastasis-specific miRNA panel including miR-377, miR-99b-5p, miR-200c and miR-196b-5p through intensive validation. These miRNAs may function as prognostic factors in patients with metastatic CRC.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.16206</identifier><identifier>PMID: 28415758</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Adult ; Aged ; Biomarkers, Tumor ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - mortality ; Colorectal Neoplasms - pathology ; Female ; Gene Expression Profiling ; Humans ; Kaplan-Meier Estimate ; Liver Neoplasms - genetics ; Liver Neoplasms - mortality ; Liver Neoplasms - secondary ; Male ; MicroRNAs - genetics ; Middle Aged ; Neoplasm Grading ; Neoplasm Staging ; Prognosis ; Research Paper ; Transcriptome</subject><ispartof>Oncotarget, 2017-05, Vol.8 (22), p.35783-35791</ispartof><rights>Copyright: © 2017 Li et al. 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-3f2f8816da6c073f3d3642f9b11f14647e987ea8203946dba597135d1e3fb7233</citedby><cites>FETCH-LOGICAL-c356t-3f2f8816da6c073f3d3642f9b11f14647e987ea8203946dba597135d1e3fb7233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482617/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482617/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28415758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Wenhua</creatorcontrib><creatorcontrib>Chang, Jinjia</creatorcontrib><creatorcontrib>Tong, Duo</creatorcontrib><creatorcontrib>Peng, Junjie</creatorcontrib><creatorcontrib>Huang, Dan</creatorcontrib><creatorcontrib>Guo, Weijian</creatorcontrib><creatorcontrib>Zhang, Wen</creatorcontrib><creatorcontrib>Li, Jin</creatorcontrib><title>Differential microRNA expression profiling in primary tumors and matched liver metastasis of patients with colorectal cancer</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>Liver metastasis is common in patients with colorectal cancer (CRC), and is correlated with poor outcome. MicroRNAs (miRNAs) are small non-coding RNAs involved in cancer development and progression, but their role in CRC liver metastasis has not been extensively investigated.
Thirteen miRNAs were deregulated in pCRCs compared to their matched liver metastases. Seventeen miRNAs were chosen for validation, which confirmed significantly reduced expression of miR-99b-5p, miR-377 and miR-200c and increased expression of miR-196b-5p in the tissue of liver metastasis. Furthermore, miR-200c and miR-196b-5p were positively correlated with shorter overall survival in pCRC patients with liver metastasis.
Firstly, affymetrix microarrays involving 1036 miRNAs were performed in two pairs of primary CRCs (pCRCs) and their matched liver metastases. Secondly, validation of the results was carried out on an independent cohort of 48 pairs of pCRCs and matched liver metastases using quantitative real-time polymerase chain reaction assay.
We discovered a pCRC liver metastasis-specific miRNA panel including miR-377, miR-99b-5p, miR-200c and miR-196b-5p through intensive validation. These miRNAs may function as prognostic factors in patients with metastatic CRC.</description><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - mortality</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - mortality</subject><subject>Liver Neoplasms - secondary</subject><subject>Male</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Staging</subject><subject>Prognosis</subject><subject>Research Paper</subject><subject>Transcriptome</subject><issn>1949-2553</issn><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUUtLBDEMLqKoqD_Ai_ToZXXaTjvtRRDfIAqi59LtpLuVmenadlcFf7xV11cIJCHJl8eH0C6pDogUjB6GwYZs4gTyARG0Eitok6hajSjnbPWPv4F2UnqsivC6kVStow0qa8IbLjfR26l3DiIM2ZsO997GcHdzjOFlFiElHwY8i8H5zg8T7D8C35v4ivO8DzFhM7S4N9lOocWdX0DEPWSTivqEg8Mzk32BTvjZ5ym2oQsRbC6DrBksxG205kyXYGdpt9DD-dn9yeXo-vbi6uT4emQZF3nEHHVSEtEaYauGOdYyUVOnxoQ4Uou6ASUbMJJWTNWiHRuuGsJ4S4C5cUMZ20JHX7iz-biH1paVoun08hgdjNf_M4Of6klYaF5LKkhTAPaXADE8zSFl3ftkoevMAGGeNJFSMcmrSpVS8lVaPplSBPczhlT6kzj9S5z-JK707P3d76fjmyb2Dl69mrY</recordid><startdate>20170530</startdate><enddate>20170530</enddate><creator>Li, Wenhua</creator><creator>Chang, Jinjia</creator><creator>Tong, Duo</creator><creator>Peng, Junjie</creator><creator>Huang, Dan</creator><creator>Guo, Weijian</creator><creator>Zhang, Wen</creator><creator>Li, Jin</creator><general>Impact Journals LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170530</creationdate><title>Differential microRNA expression profiling in primary tumors and matched liver metastasis of patients with colorectal cancer</title><author>Li, Wenhua ; Chang, Jinjia ; Tong, Duo ; Peng, Junjie ; Huang, Dan ; Guo, Weijian ; Zhang, Wen ; Li, Jin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-3f2f8816da6c073f3d3642f9b11f14647e987ea8203946dba597135d1e3fb7233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - mortality</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Liver Neoplasms - genetics</topic><topic>Liver Neoplasms - mortality</topic><topic>Liver Neoplasms - secondary</topic><topic>Male</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Staging</topic><topic>Prognosis</topic><topic>Research Paper</topic><topic>Transcriptome</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Wenhua</creatorcontrib><creatorcontrib>Chang, Jinjia</creatorcontrib><creatorcontrib>Tong, Duo</creatorcontrib><creatorcontrib>Peng, Junjie</creatorcontrib><creatorcontrib>Huang, Dan</creatorcontrib><creatorcontrib>Guo, Weijian</creatorcontrib><creatorcontrib>Zhang, Wen</creatorcontrib><creatorcontrib>Li, Jin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Wenhua</au><au>Chang, Jinjia</au><au>Tong, Duo</au><au>Peng, Junjie</au><au>Huang, Dan</au><au>Guo, Weijian</au><au>Zhang, Wen</au><au>Li, Jin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential microRNA expression profiling in primary tumors and matched liver metastasis of patients with colorectal cancer</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2017-05-30</date><risdate>2017</risdate><volume>8</volume><issue>22</issue><spage>35783</spage><epage>35791</epage><pages>35783-35791</pages><issn>1949-2553</issn><eissn>1949-2553</eissn><abstract>Liver metastasis is common in patients with colorectal cancer (CRC), and is correlated with poor outcome. MicroRNAs (miRNAs) are small non-coding RNAs involved in cancer development and progression, but their role in CRC liver metastasis has not been extensively investigated.
Thirteen miRNAs were deregulated in pCRCs compared to their matched liver metastases. Seventeen miRNAs were chosen for validation, which confirmed significantly reduced expression of miR-99b-5p, miR-377 and miR-200c and increased expression of miR-196b-5p in the tissue of liver metastasis. Furthermore, miR-200c and miR-196b-5p were positively correlated with shorter overall survival in pCRC patients with liver metastasis.
Firstly, affymetrix microarrays involving 1036 miRNAs were performed in two pairs of primary CRCs (pCRCs) and their matched liver metastases. Secondly, validation of the results was carried out on an independent cohort of 48 pairs of pCRCs and matched liver metastases using quantitative real-time polymerase chain reaction assay.
We discovered a pCRC liver metastasis-specific miRNA panel including miR-377, miR-99b-5p, miR-200c and miR-196b-5p through intensive validation. These miRNAs may function as prognostic factors in patients with metastatic CRC.</abstract><cop>United States</cop><pub>Impact Journals LLC</pub><pmid>28415758</pmid><doi>10.18632/oncotarget.16206</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Aged Biomarkers, Tumor Colorectal Neoplasms - genetics Colorectal Neoplasms - mortality Colorectal Neoplasms - pathology Female Gene Expression Profiling Humans Kaplan-Meier Estimate Liver Neoplasms - genetics Liver Neoplasms - mortality Liver Neoplasms - secondary Male MicroRNAs - genetics Middle Aged Neoplasm Grading Neoplasm Staging Prognosis Research Paper Transcriptome |
title | Differential microRNA expression profiling in primary tumors and matched liver metastasis of patients with colorectal cancer |
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