Line-scanning hyperspectral imaging based on structured illumination optical sectioning
Line-scanning hyperspectral imaging (LHSI) is known to have a higher acquisition rate but lower sectioning capability than point-scanning hyperspectral imaging. To further increase the axial imaging contrast of LHSI, structured illumination was integrated into line excitation to remove the off-focus...
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| Veröffentlicht in: | Biomedical optics express 2017-06, Vol.8 (6), p.3005-3016 |
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| container_title | Biomedical optics express |
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| creator | Hsu, Yu John Chen, Chih-Chiang Huang, Chien-Hsiang Yeh, Chia-Hua Liu, Li-Ying Chen, Szu-Yu |
| description | Line-scanning hyperspectral imaging (LHSI) is known to have a higher acquisition rate but lower sectioning capability than point-scanning hyperspectral imaging. To further increase the axial imaging contrast of LHSI, structured illumination was integrated into line excitation to remove the off-focus and scattered on-focus fluorescence signals. In an unsectioned leaf, the imaging contrast can be enhanced by 8 times, while in sectioned mouse skin tissues, a 4.5-fold enhancement can be achieved. With a spectral resolution of 1.15 nm, the fluorophores with seriously-overlapped spectra was proved to be separated without cross-talk by applying linear unmixing to the recorded spectral information. |
| doi_str_mv | 10.1364/BOE.8.003005 |
| format | Article |
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| title | Line-scanning hyperspectral imaging based on structured illumination optical sectioning |
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