Sensitive Analysis of Protein Adsorption to Colloidal Gold by Differential Centrifugal Sedimentation

It is demonstrated that the adsorption of bovine serum albumin (BSA) to aqueous gold colloids can be quantified with molecular resolution by differential centrifugal sedimentation (DCS). This method separates colloidal particles of comparable density by mass. When proteins adsorb to the nanoparticle...

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Veröffentlicht in:	Analytical chemistry (Washington) 2017-06, Vol.89 (12), p.6807-6814
Hauptverfasser:	Davidson, Adam M, Brust, Mathias, Cooper, David L, Volk, Martin
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Analytical chemistry Animals Bovine serum albumin Cattle Chemistry Colloids Gold Gold Colloid - chemistry Ligands Nanoparticles Nanoparticles - chemistry Polyethylene glycol Polyethylene Glycols - chemistry Protein adsorption Proteins Sedimentation Serum albumin Serum Albumin, Bovine - chemistry Sulfhydryl Compounds - chemistry Surface chemistry Ultracentrifugation - methods
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