Interferon priming is necessary but not sufficient to turn on a migratory dendritic cell program in lupus monocytes
Blood monocytes from children with systemic lupus erythematosus (SLE) behave like dendritic cells (DCs), and SLE serum induces healthy monocytes to differentiate into DCs in a type I Interferon (IFN)-dependent manner. Here, we found that these monocytes display significant transcriptional changes, i...
Gespeichert in:
| Veröffentlicht in: | The Journal of immunology (1950) 2014-05, Vol.192 (12), p.5586-5598 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5598 |
|---|---|
| container_issue | 12 |
| container_start_page | 5586 |
| container_title | The Journal of immunology (1950) |
| container_volume | 192 |
| creator | Rodriguez-Pla, Alicia Patel, Pinakeen Maecker, Holden T. Rossello-Urgell, Jose Baldwin, Nicole Bennett, Lynda Cantrell, Victoria Baisch, Jeanine Punaro, Marilynn Gotte, Alisa Nassi, Lorien Wright, Tracey Palucka, Anna Karolina Banchereau, Jacques Pascual, Virginia |
| description | Blood monocytes from children with systemic lupus erythematosus (SLE) behave like dendritic cells (DCs), and SLE serum induces healthy monocytes to differentiate into DCs in a type I Interferon (IFN)-dependent manner. Here, we found that these monocytes display significant transcriptional changes, including a prominent IFN signature, compared to healthy controls. Few of those changes, however, explain DC function. Exposure to allogeneic T cells
in vitro
reprograms SLE monocytes to acquire DC phenotype and function, and this correlates with both IFN-inducible (
IP10
) and pro-inflammatory cytokine (
IL1b
and
IL6
) expression. Furthermore, we found that both IFN and SLE serum induce the upregulation of
CCR7
transcription in these cells. CCR7 protein expression requires however a second signal provided by TLR agonists such as LPS. Thus, SLE serum “primes” a subset of monocytes to readily ( |
| doi_str_mv | 10.4049/jimmunol.1301319 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmedcentral</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5467885</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>pubmedcentral_primary_oai_pubmedcentral_nih_gov_5467885</sourcerecordid><originalsourceid>FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_54678853</originalsourceid><addsrcrecordid>eNqljrlOxDAURS0EYoalp3w_kMFOHCdpaBAIevrI4zjhjeznyAvS_D1BoqGmusW5G2MPgh8kl8PjCb0vFNxBNFw0Yrhge9G2vFKKq0u257yuK9GpbsduUjpxzhWv5TXb1bKvBynknqV3yjbONgaCNaJHWgATkDU2JR3PcCwZKGRIZZ7RoKUMOUAukWCLaPC4RJ3D5pwsTREzGjDWua0tbMQDEriylgQ-UDDnbNMdu5q1S_b-V2_Z0-vLx_NbtZajt5PZNqJ248-d7cEYNI5_CeHnuISvsZWq6_u2-XfBN_IabdU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Interferon priming is necessary but not sufficient to turn on a migratory dendritic cell program in lupus monocytes</title><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Rodriguez-Pla, Alicia ; Patel, Pinakeen ; Maecker, Holden T. ; Rossello-Urgell, Jose ; Baldwin, Nicole ; Bennett, Lynda ; Cantrell, Victoria ; Baisch, Jeanine ; Punaro, Marilynn ; Gotte, Alisa ; Nassi, Lorien ; Wright, Tracey ; Palucka, Anna Karolina ; Banchereau, Jacques ; Pascual, Virginia</creator><creatorcontrib>Rodriguez-Pla, Alicia ; Patel, Pinakeen ; Maecker, Holden T. ; Rossello-Urgell, Jose ; Baldwin, Nicole ; Bennett, Lynda ; Cantrell, Victoria ; Baisch, Jeanine ; Punaro, Marilynn ; Gotte, Alisa ; Nassi, Lorien ; Wright, Tracey ; Palucka, Anna Karolina ; Banchereau, Jacques ; Pascual, Virginia</creatorcontrib><description>Blood monocytes from children with systemic lupus erythematosus (SLE) behave like dendritic cells (DCs), and SLE serum induces healthy monocytes to differentiate into DCs in a type I Interferon (IFN)-dependent manner. Here, we found that these monocytes display significant transcriptional changes, including a prominent IFN signature, compared to healthy controls. Few of those changes, however, explain DC function. Exposure to allogeneic T cells
in vitro
reprograms SLE monocytes to acquire DC phenotype and function, and this correlates with both IFN-inducible (
IP10
) and pro-inflammatory cytokine (
IL1b
and
IL6
) expression. Furthermore, we found that both IFN and SLE serum induce the upregulation of
CCR7
transcription in these cells. CCR7 protein expression requires however a second signal provided by TLR agonists such as LPS. Thus, SLE serum “primes” a subset of monocytes to readily (<24h) respond to TLR agonists and acquire migratory DC properties. Our findings might explain how microbial infections exacerbate lupus.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.1301319</identifier><identifier>PMID: 24829414</identifier><language>eng</language><ispartof>The Journal of immunology (1950), 2014-05, Vol.192 (12), p.5586-5598</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids></links><search><creatorcontrib>Rodriguez-Pla, Alicia</creatorcontrib><creatorcontrib>Patel, Pinakeen</creatorcontrib><creatorcontrib>Maecker, Holden T.</creatorcontrib><creatorcontrib>Rossello-Urgell, Jose</creatorcontrib><creatorcontrib>Baldwin, Nicole</creatorcontrib><creatorcontrib>Bennett, Lynda</creatorcontrib><creatorcontrib>Cantrell, Victoria</creatorcontrib><creatorcontrib>Baisch, Jeanine</creatorcontrib><creatorcontrib>Punaro, Marilynn</creatorcontrib><creatorcontrib>Gotte, Alisa</creatorcontrib><creatorcontrib>Nassi, Lorien</creatorcontrib><creatorcontrib>Wright, Tracey</creatorcontrib><creatorcontrib>Palucka, Anna Karolina</creatorcontrib><creatorcontrib>Banchereau, Jacques</creatorcontrib><creatorcontrib>Pascual, Virginia</creatorcontrib><title>Interferon priming is necessary but not sufficient to turn on a migratory dendritic cell program in lupus monocytes</title><title>The Journal of immunology (1950)</title><description>Blood monocytes from children with systemic lupus erythematosus (SLE) behave like dendritic cells (DCs), and SLE serum induces healthy monocytes to differentiate into DCs in a type I Interferon (IFN)-dependent manner. Here, we found that these monocytes display significant transcriptional changes, including a prominent IFN signature, compared to healthy controls. Few of those changes, however, explain DC function. Exposure to allogeneic T cells
in vitro
reprograms SLE monocytes to acquire DC phenotype and function, and this correlates with both IFN-inducible (
IP10
) and pro-inflammatory cytokine (
IL1b
and
IL6
) expression. Furthermore, we found that both IFN and SLE serum induce the upregulation of
CCR7
transcription in these cells. CCR7 protein expression requires however a second signal provided by TLR agonists such as LPS. Thus, SLE serum “primes” a subset of monocytes to readily (<24h) respond to TLR agonists and acquire migratory DC properties. Our findings might explain how microbial infections exacerbate lupus.</description><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqljrlOxDAURS0EYoalp3w_kMFOHCdpaBAIevrI4zjhjeznyAvS_D1BoqGmusW5G2MPgh8kl8PjCb0vFNxBNFw0Yrhge9G2vFKKq0u257yuK9GpbsduUjpxzhWv5TXb1bKvBynknqV3yjbONgaCNaJHWgATkDU2JR3PcCwZKGRIZZ7RoKUMOUAukWCLaPC4RJ3D5pwsTREzGjDWua0tbMQDEriylgQ-UDDnbNMdu5q1S_b-V2_Z0-vLx_NbtZajt5PZNqJ248-d7cEYNI5_CeHnuISvsZWq6_u2-XfBN_IabdU</recordid><startdate>20140514</startdate><enddate>20140514</enddate><creator>Rodriguez-Pla, Alicia</creator><creator>Patel, Pinakeen</creator><creator>Maecker, Holden T.</creator><creator>Rossello-Urgell, Jose</creator><creator>Baldwin, Nicole</creator><creator>Bennett, Lynda</creator><creator>Cantrell, Victoria</creator><creator>Baisch, Jeanine</creator><creator>Punaro, Marilynn</creator><creator>Gotte, Alisa</creator><creator>Nassi, Lorien</creator><creator>Wright, Tracey</creator><creator>Palucka, Anna Karolina</creator><creator>Banchereau, Jacques</creator><creator>Pascual, Virginia</creator><scope>5PM</scope></search><sort><creationdate>20140514</creationdate><title>Interferon priming is necessary but not sufficient to turn on a migratory dendritic cell program in lupus monocytes</title><author>Rodriguez-Pla, Alicia ; Patel, Pinakeen ; Maecker, Holden T. ; Rossello-Urgell, Jose ; Baldwin, Nicole ; Bennett, Lynda ; Cantrell, Victoria ; Baisch, Jeanine ; Punaro, Marilynn ; Gotte, Alisa ; Nassi, Lorien ; Wright, Tracey ; Palucka, Anna Karolina ; Banchereau, Jacques ; Pascual, Virginia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_54678853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodriguez-Pla, Alicia</creatorcontrib><creatorcontrib>Patel, Pinakeen</creatorcontrib><creatorcontrib>Maecker, Holden T.</creatorcontrib><creatorcontrib>Rossello-Urgell, Jose</creatorcontrib><creatorcontrib>Baldwin, Nicole</creatorcontrib><creatorcontrib>Bennett, Lynda</creatorcontrib><creatorcontrib>Cantrell, Victoria</creatorcontrib><creatorcontrib>Baisch, Jeanine</creatorcontrib><creatorcontrib>Punaro, Marilynn</creatorcontrib><creatorcontrib>Gotte, Alisa</creatorcontrib><creatorcontrib>Nassi, Lorien</creatorcontrib><creatorcontrib>Wright, Tracey</creatorcontrib><creatorcontrib>Palucka, Anna Karolina</creatorcontrib><creatorcontrib>Banchereau, Jacques</creatorcontrib><creatorcontrib>Pascual, Virginia</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodriguez-Pla, Alicia</au><au>Patel, Pinakeen</au><au>Maecker, Holden T.</au><au>Rossello-Urgell, Jose</au><au>Baldwin, Nicole</au><au>Bennett, Lynda</au><au>Cantrell, Victoria</au><au>Baisch, Jeanine</au><au>Punaro, Marilynn</au><au>Gotte, Alisa</au><au>Nassi, Lorien</au><au>Wright, Tracey</au><au>Palucka, Anna Karolina</au><au>Banchereau, Jacques</au><au>Pascual, Virginia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interferon priming is necessary but not sufficient to turn on a migratory dendritic cell program in lupus monocytes</atitle><jtitle>The Journal of immunology (1950)</jtitle><date>2014-05-14</date><risdate>2014</risdate><volume>192</volume><issue>12</issue><spage>5586</spage><epage>5598</epage><pages>5586-5598</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Blood monocytes from children with systemic lupus erythematosus (SLE) behave like dendritic cells (DCs), and SLE serum induces healthy monocytes to differentiate into DCs in a type I Interferon (IFN)-dependent manner. Here, we found that these monocytes display significant transcriptional changes, including a prominent IFN signature, compared to healthy controls. Few of those changes, however, explain DC function. Exposure to allogeneic T cells
in vitro
reprograms SLE monocytes to acquire DC phenotype and function, and this correlates with both IFN-inducible (
IP10
) and pro-inflammatory cytokine (
IL1b
and
IL6
) expression. Furthermore, we found that both IFN and SLE serum induce the upregulation of
CCR7
transcription in these cells. CCR7 protein expression requires however a second signal provided by TLR agonists such as LPS. Thus, SLE serum “primes” a subset of monocytes to readily (<24h) respond to TLR agonists and acquire migratory DC properties. Our findings might explain how microbial infections exacerbate lupus.</abstract><pmid>24829414</pmid><doi>10.4049/jimmunol.1301319</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 2014-05, Vol.192 (12), p.5586-5598 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5467885 |
| source | EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| title | Interferon priming is necessary but not sufficient to turn on a migratory dendritic cell program in lupus monocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T06%3A30%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmedcentral&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Interferon%20priming%20is%20necessary%20but%20not%20sufficient%20to%20turn%20on%20a%20migratory%20dendritic%20cell%20program%20in%20lupus%20monocytes&rft.jtitle=The%20Journal%20of%20immunology%20(1950)&rft.au=Rodriguez-Pla,%20Alicia&rft.date=2014-05-14&rft.volume=192&rft.issue=12&rft.spage=5586&rft.epage=5598&rft.pages=5586-5598&rft.issn=0022-1767&rft.eissn=1550-6606&rft_id=info:doi/10.4049/jimmunol.1301319&rft_dat=%3Cpubmedcentral%3Epubmedcentral_primary_oai_pubmedcentral_nih_gov_5467885%3C/pubmedcentral%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/24829414&rfr_iscdi=true |