Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel
Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs i...
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| Veröffentlicht in: | Stem cells translational medicine 2017-05, Vol.6 (5), p.1412-1423 |
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| container_title | Stem cells translational medicine |
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| creator | Zhu, Pei Zhou, Yalu Wu, Furen Hong, Yuanfan Wang, Xin Shekhawat, Gajendra Mosenson, Jeffrey Wu, Wen‐Shu |
| description | Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three‐dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell‐based therapies for various muscle disorders. Stem Cells Translational Medicine 2017;6:1412–1423 |
| doi_str_mv | 10.1002/sctm.16-0427 |
| format | Article |
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Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three‐dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell‐based therapies for various muscle disorders. 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Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 The Authors S C T M published by Wiley Periodicals, Inc. on behalf of AlphaMed Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4847-36ac608dcab2dfe524dcc024848e690f97487edf9fe56fb9a82d008dffe871e13</citedby><cites>FETCH-LOGICAL-c4847-36ac608dcab2dfe524dcc024848e690f97487edf9fe56fb9a82d008dffe871e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442710/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442710/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28244269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Pei</creatorcontrib><creatorcontrib>Zhou, Yalu</creatorcontrib><creatorcontrib>Wu, Furen</creatorcontrib><creatorcontrib>Hong, Yuanfan</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Shekhawat, Gajendra</creatorcontrib><creatorcontrib>Mosenson, Jeffrey</creatorcontrib><creatorcontrib>Wu, Wen‐Shu</creatorcontrib><title>Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel</title><title>Stem cells translational medicine</title><addtitle>Stem Cells Transl Med</addtitle><description>Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three‐dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell‐based therapies for various muscle disorders. 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| subjects | Cell culture Cell cycle CRISPR Data analysis Fibrin Gene expression Genomes Health aspects Kinases Laboratories Muscle stem cells Muscles Musculoskeletal system Myogenesis Myogenic progenitor cells Nanotechnology Salmon Satellite cells Skeletal muscle Stem cell transplantation Stem cells Three‐dimensional fibrin gel Tissue‐Specific Progenitor and Stem Cells Translational s and Reviews Transplantation |
| title | Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three‐Dimensional Fibrin Gel |
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