CRISPR/Cas9 gene drives in genetically variable and nonrandomly mating wild populations
Synthetic gene drives based on CRISPR/Cas9 have the potential to control, alter, or suppress populations of crop pests and disease vectors, but it is unclear how they will function in wild populations. Using genetic data from four populations of the flour beetle , we show that most populations harbo...
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| Veröffentlicht in: | Science advances 2017-05, Vol.3 (5), p.e1601910-e1601910 |
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| container_end_page | e1601910 |
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| container_issue | 5 |
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| container_title | Science advances |
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| creator | Drury, Douglas W Dapper, Amy L Siniard, Dylan J Zentner, Gabriel E Wade, Michael J |
| description | Synthetic gene drives based on CRISPR/Cas9 have the potential to control, alter, or suppress populations of crop pests and disease vectors, but it is unclear how they will function in wild populations. Using genetic data from four populations of the flour beetle
, we show that most populations harbor genetic variants in Cas9 target sites, some of which would render them immune to drive (ITD). We show that even a rare ITD allele can reduce or eliminate the efficacy of a CRISPR/Cas9-based synthetic gene drive. This effect is equivalent to and accentuated by mild inbreeding, which is a characteristic of many disease-vectoring arthropods. We conclude that designing such drives will require characterization of genetic variability and the mating system within and among targeted populations. |
| doi_str_mv | 10.1126/sciadv.1601910 |
| format | Article |
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| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals CRISPR-Cas Systems Genetic Variation Models, Genetic Population Genetics Reproduction - genetics SciAdv r-articles Tribolium - genetics |
| title | CRISPR/Cas9 gene drives in genetically variable and nonrandomly mating wild populations |
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