In vivo application of Granulocyte-Macrophage Colony-stimulating Factor enhances postoperative qualitative monocytic function
Granulocyte macrophage colony-stimulating factor (GM-CSF) can be used as a potent stimulator for immune suppressed patients as defined by a decrease of human leukocyte antigen-D related expression on monocytes (mHLA-DR) after surgery. However, the exact role of GM-CSF on monocytic and T cell functio...
Gespeichert in:
| Veröffentlicht in: | International journal of medical sciences 2017-01, Vol.14 (4), p.367-375 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 375 |
|---|---|
| container_issue | 4 |
| container_start_page | 367 |
| container_title | International journal of medical sciences |
| container_volume | 14 |
| creator | Lachmann, Gunnar Kurth, Johannes von Haefen, Clarissa Yuerek, Fatima Wernecke, Klaus-Dieter Spies, Claudia |
| description | Granulocyte macrophage colony-stimulating factor (GM-CSF) can be used as a potent stimulator for immune suppressed patients as defined by a decrease of human leukocyte antigen-D related expression on monocytes (mHLA-DR) after surgery. However, the exact role of GM-CSF on monocytic and T cell function is unclear.
In this retrospective randomized controlled trial (RCT) subgroup analysis, monocytic respectively T cell function and T cell subspecies of 20 immune suppressed (i.e. mHLA-DR levels below 10,000 monoclonal antibodies (mAb) per cell at the first day after surgery) patients after esophageal or pancreatic resection were analyzed. Each 10 patients received either GM-CSF (250 μg/m²/d) or placebo for a maximum of three consecutive days if mHLA-DR levels remained below 10,000 mAb per cell. mHLA-DR and further parameters of immune function were measured preoperatively (
) until day 5 after surgery (
). Statistical analyses were performed using nonparametric statistical procedures.
In multivariate analysis, mHLA-DR significantly differed between the groups (p < 0.001). mHLA-DR was increased on
(p < 0.001) and
(p = 0.002) after GM-CSF application. Tumor necrosis factor-α (TNF-α) release of lipopolysaccharide (LPS) stimulated monocytes multivariately significantly differed between the groups (p < 0.008) and was increased in the GM-CSF group on
(p < 0.001) and
(p = 0.046). Th17/regulatory T (Treg) cell ratio was higher after GM-CSF treatment on
(p = 0.041). No differences were seen in lymphocytes and T helper cell (Th)1/Th2 specific cytokine production after T cell stimulation with Concanavalin (Con) A between the groups.
Postoperative application of GM-CSF significantly enhanced qualitative monocytic function by increased mHLA-DR and TNF-α release after LPS stimulation and apparently enhanced Th17/Treg ratio. Clinical trial registered with www.controlled-trials.com (ISRCTN27114642) 05 December 2008. |
| doi_str_mv | 10.7150/ijms.18288 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5436479</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1903439794</sourcerecordid><originalsourceid>FETCH-LOGICAL-c378t-29f6c6e52f52ae3db0420730a563c349911dae47dad701bc931312fb2161f00c3</originalsourceid><addsrcrecordid>eNpVkU9LJDEQxcPisrruXvwAkqMstOZfdzoXQQZ1BWUveg416fRMJJ20SXpgDn53e3ZccU9V8H68V9RD6ISSc0lrcuGeh3xOW9a2X9ARFUJVVBF58Gk_RN9zfiaEMy7pN3TI2rrmtFFH6PUu4I3bRAzj6J2B4mLAsce3CcLko9kWWz2ASXFcw8riRfQxbKtc3DD5GQ4rfAOmxIRtWEMwNuMx5hJHm2Z1Y_HLBN6V_T7EsDN0BvdTMLukH-hrDz7bn-_zGD3dXD8uflf3f27vFlf3leGyLRVTfWMaW7O-ZmB5tySCEckJ1A03XChFaQdWyA46SejSKE45Zf2S0Yb2hBh-jC73vuO0HGxnbCgJvB6TGyBtdQSn_1eCW-tV3Oha8EZINRucvRuk-DLZXPTgsrHeQ7Bxynp-MhdcSSVm9NcenZ-Wc7L9RwwleteX3vWl__Y1w6efD_tA_xXE3wBg-5X3</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1903439794</pqid></control><display><type>article</type><title>In vivo application of Granulocyte-Macrophage Colony-stimulating Factor enhances postoperative qualitative monocytic function</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>PubMed Central</source><creator>Lachmann, Gunnar ; Kurth, Johannes ; von Haefen, Clarissa ; Yuerek, Fatima ; Wernecke, Klaus-Dieter ; Spies, Claudia</creator><creatorcontrib>Lachmann, Gunnar ; Kurth, Johannes ; von Haefen, Clarissa ; Yuerek, Fatima ; Wernecke, Klaus-Dieter ; Spies, Claudia</creatorcontrib><description>Granulocyte macrophage colony-stimulating factor (GM-CSF) can be used as a potent stimulator for immune suppressed patients as defined by a decrease of human leukocyte antigen-D related expression on monocytes (mHLA-DR) after surgery. However, the exact role of GM-CSF on monocytic and T cell function is unclear.
In this retrospective randomized controlled trial (RCT) subgroup analysis, monocytic respectively T cell function and T cell subspecies of 20 immune suppressed (i.e. mHLA-DR levels below 10,000 monoclonal antibodies (mAb) per cell at the first day after surgery) patients after esophageal or pancreatic resection were analyzed. Each 10 patients received either GM-CSF (250 μg/m²/d) or placebo for a maximum of three consecutive days if mHLA-DR levels remained below 10,000 mAb per cell. mHLA-DR and further parameters of immune function were measured preoperatively (
) until day 5 after surgery (
). Statistical analyses were performed using nonparametric statistical procedures.
In multivariate analysis, mHLA-DR significantly differed between the groups (p < 0.001). mHLA-DR was increased on
(p < 0.001) and
(p = 0.002) after GM-CSF application. Tumor necrosis factor-α (TNF-α) release of lipopolysaccharide (LPS) stimulated monocytes multivariately significantly differed between the groups (p < 0.008) and was increased in the GM-CSF group on
(p < 0.001) and
(p = 0.046). Th17/regulatory T (Treg) cell ratio was higher after GM-CSF treatment on
(p = 0.041). No differences were seen in lymphocytes and T helper cell (Th)1/Th2 specific cytokine production after T cell stimulation with Concanavalin (Con) A between the groups.
Postoperative application of GM-CSF significantly enhanced qualitative monocytic function by increased mHLA-DR and TNF-α release after LPS stimulation and apparently enhanced Th17/Treg ratio. Clinical trial registered with www.controlled-trials.com (ISRCTN27114642) 05 December 2008.</description><identifier>ISSN: 1449-1907</identifier><identifier>EISSN: 1449-1907</identifier><identifier>DOI: 10.7150/ijms.18288</identifier><identifier>PMID: 28553169</identifier><language>eng</language><publisher>Australia: Ivyspring International Publisher</publisher><subject>Adult ; Esophagus - immunology ; Esophagus - pathology ; Esophagus - surgery ; Female ; Flow Cytometry ; Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage ; Granulocyte-Macrophage Colony-Stimulating Factor - immunology ; HLA-DR Antigens - immunology ; Humans ; Interferon-gamma - immunology ; Lipopolysaccharides - administration & dosage ; Male ; Monocytes - drug effects ; Monocytes - immunology ; Pancreas - immunology ; Pancreas - pathology ; Pancreas - surgery ; Postoperative Complications - drug therapy ; Postoperative Complications - genetics ; Postoperative Complications - physiopathology ; Research Paper ; T-Lymphocyte Subsets - immunology ; T-Lymphocytes, Regulatory - drug effects ; T-Lymphocytes, Regulatory - immunology ; Th17 Cells - drug effects ; Th17 Cells - immunology ; Tumor Necrosis Factor-alpha - immunology</subject><ispartof>International journal of medical sciences, 2017-01, Vol.14 (4), p.367-375</ispartof><rights>Ivyspring International Publisher 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-29f6c6e52f52ae3db0420730a563c349911dae47dad701bc931312fb2161f00c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436479/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436479/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27911,27912,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28553169$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lachmann, Gunnar</creatorcontrib><creatorcontrib>Kurth, Johannes</creatorcontrib><creatorcontrib>von Haefen, Clarissa</creatorcontrib><creatorcontrib>Yuerek, Fatima</creatorcontrib><creatorcontrib>Wernecke, Klaus-Dieter</creatorcontrib><creatorcontrib>Spies, Claudia</creatorcontrib><title>In vivo application of Granulocyte-Macrophage Colony-stimulating Factor enhances postoperative qualitative monocytic function</title><title>International journal of medical sciences</title><addtitle>Int J Med Sci</addtitle><description>Granulocyte macrophage colony-stimulating factor (GM-CSF) can be used as a potent stimulator for immune suppressed patients as defined by a decrease of human leukocyte antigen-D related expression on monocytes (mHLA-DR) after surgery. However, the exact role of GM-CSF on monocytic and T cell function is unclear.
In this retrospective randomized controlled trial (RCT) subgroup analysis, monocytic respectively T cell function and T cell subspecies of 20 immune suppressed (i.e. mHLA-DR levels below 10,000 monoclonal antibodies (mAb) per cell at the first day after surgery) patients after esophageal or pancreatic resection were analyzed. Each 10 patients received either GM-CSF (250 μg/m²/d) or placebo for a maximum of three consecutive days if mHLA-DR levels remained below 10,000 mAb per cell. mHLA-DR and further parameters of immune function were measured preoperatively (
) until day 5 after surgery (
). Statistical analyses were performed using nonparametric statistical procedures.
In multivariate analysis, mHLA-DR significantly differed between the groups (p < 0.001). mHLA-DR was increased on
(p < 0.001) and
(p = 0.002) after GM-CSF application. Tumor necrosis factor-α (TNF-α) release of lipopolysaccharide (LPS) stimulated monocytes multivariately significantly differed between the groups (p < 0.008) and was increased in the GM-CSF group on
(p < 0.001) and
(p = 0.046). Th17/regulatory T (Treg) cell ratio was higher after GM-CSF treatment on
(p = 0.041). No differences were seen in lymphocytes and T helper cell (Th)1/Th2 specific cytokine production after T cell stimulation with Concanavalin (Con) A between the groups.
Postoperative application of GM-CSF significantly enhanced qualitative monocytic function by increased mHLA-DR and TNF-α release after LPS stimulation and apparently enhanced Th17/Treg ratio. Clinical trial registered with www.controlled-trials.com (ISRCTN27114642) 05 December 2008.</description><subject>Adult</subject><subject>Esophagus - immunology</subject><subject>Esophagus - pathology</subject><subject>Esophagus - surgery</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - immunology</subject><subject>HLA-DR Antigens - immunology</subject><subject>Humans</subject><subject>Interferon-gamma - immunology</subject><subject>Lipopolysaccharides - administration & dosage</subject><subject>Male</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - immunology</subject><subject>Pancreas - immunology</subject><subject>Pancreas - pathology</subject><subject>Pancreas - surgery</subject><subject>Postoperative Complications - drug therapy</subject><subject>Postoperative Complications - genetics</subject><subject>Postoperative Complications - physiopathology</subject><subject>Research Paper</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocytes, Regulatory - drug effects</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>Th17 Cells - drug effects</subject><subject>Th17 Cells - immunology</subject><subject>Tumor Necrosis Factor-alpha - immunology</subject><issn>1449-1907</issn><issn>1449-1907</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9LJDEQxcPisrruXvwAkqMstOZfdzoXQQZ1BWUveg416fRMJJ20SXpgDn53e3ZccU9V8H68V9RD6ISSc0lrcuGeh3xOW9a2X9ARFUJVVBF58Gk_RN9zfiaEMy7pN3TI2rrmtFFH6PUu4I3bRAzj6J2B4mLAsce3CcLko9kWWz2ASXFcw8riRfQxbKtc3DD5GQ4rfAOmxIRtWEMwNuMx5hJHm2Z1Y_HLBN6V_T7EsDN0BvdTMLukH-hrDz7bn-_zGD3dXD8uflf3f27vFlf3leGyLRVTfWMaW7O-ZmB5tySCEckJ1A03XChFaQdWyA46SejSKE45Zf2S0Yb2hBh-jC73vuO0HGxnbCgJvB6TGyBtdQSn_1eCW-tV3Oha8EZINRucvRuk-DLZXPTgsrHeQ7Bxynp-MhdcSSVm9NcenZ-Wc7L9RwwleteX3vWl__Y1w6efD_tA_xXE3wBg-5X3</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Lachmann, Gunnar</creator><creator>Kurth, Johannes</creator><creator>von Haefen, Clarissa</creator><creator>Yuerek, Fatima</creator><creator>Wernecke, Klaus-Dieter</creator><creator>Spies, Claudia</creator><general>Ivyspring International Publisher</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170101</creationdate><title>In vivo application of Granulocyte-Macrophage Colony-stimulating Factor enhances postoperative qualitative monocytic function</title><author>Lachmann, Gunnar ; Kurth, Johannes ; von Haefen, Clarissa ; Yuerek, Fatima ; Wernecke, Klaus-Dieter ; Spies, Claudia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-29f6c6e52f52ae3db0420730a563c349911dae47dad701bc931312fb2161f00c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Esophagus - immunology</topic><topic>Esophagus - pathology</topic><topic>Esophagus - surgery</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - immunology</topic><topic>HLA-DR Antigens - immunology</topic><topic>Humans</topic><topic>Interferon-gamma - immunology</topic><topic>Lipopolysaccharides - administration & dosage</topic><topic>Male</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - immunology</topic><topic>Pancreas - immunology</topic><topic>Pancreas - pathology</topic><topic>Pancreas - surgery</topic><topic>Postoperative Complications - drug therapy</topic><topic>Postoperative Complications - genetics</topic><topic>Postoperative Complications - physiopathology</topic><topic>Research Paper</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocytes, Regulatory - drug effects</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>Th17 Cells - drug effects</topic><topic>Th17 Cells - immunology</topic><topic>Tumor Necrosis Factor-alpha - immunology</topic><toplevel>online_resources</toplevel><creatorcontrib>Lachmann, Gunnar</creatorcontrib><creatorcontrib>Kurth, Johannes</creatorcontrib><creatorcontrib>von Haefen, Clarissa</creatorcontrib><creatorcontrib>Yuerek, Fatima</creatorcontrib><creatorcontrib>Wernecke, Klaus-Dieter</creatorcontrib><creatorcontrib>Spies, Claudia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lachmann, Gunnar</au><au>Kurth, Johannes</au><au>von Haefen, Clarissa</au><au>Yuerek, Fatima</au><au>Wernecke, Klaus-Dieter</au><au>Spies, Claudia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo application of Granulocyte-Macrophage Colony-stimulating Factor enhances postoperative qualitative monocytic function</atitle><jtitle>International journal of medical sciences</jtitle><addtitle>Int J Med Sci</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>14</volume><issue>4</issue><spage>367</spage><epage>375</epage><pages>367-375</pages><issn>1449-1907</issn><eissn>1449-1907</eissn><abstract>Granulocyte macrophage colony-stimulating factor (GM-CSF) can be used as a potent stimulator for immune suppressed patients as defined by a decrease of human leukocyte antigen-D related expression on monocytes (mHLA-DR) after surgery. However, the exact role of GM-CSF on monocytic and T cell function is unclear.
In this retrospective randomized controlled trial (RCT) subgroup analysis, monocytic respectively T cell function and T cell subspecies of 20 immune suppressed (i.e. mHLA-DR levels below 10,000 monoclonal antibodies (mAb) per cell at the first day after surgery) patients after esophageal or pancreatic resection were analyzed. Each 10 patients received either GM-CSF (250 μg/m²/d) or placebo for a maximum of three consecutive days if mHLA-DR levels remained below 10,000 mAb per cell. mHLA-DR and further parameters of immune function were measured preoperatively (
) until day 5 after surgery (
). Statistical analyses were performed using nonparametric statistical procedures.
In multivariate analysis, mHLA-DR significantly differed between the groups (p < 0.001). mHLA-DR was increased on
(p < 0.001) and
(p = 0.002) after GM-CSF application. Tumor necrosis factor-α (TNF-α) release of lipopolysaccharide (LPS) stimulated monocytes multivariately significantly differed between the groups (p < 0.008) and was increased in the GM-CSF group on
(p < 0.001) and
(p = 0.046). Th17/regulatory T (Treg) cell ratio was higher after GM-CSF treatment on
(p = 0.041). No differences were seen in lymphocytes and T helper cell (Th)1/Th2 specific cytokine production after T cell stimulation with Concanavalin (Con) A between the groups.
Postoperative application of GM-CSF significantly enhanced qualitative monocytic function by increased mHLA-DR and TNF-α release after LPS stimulation and apparently enhanced Th17/Treg ratio. Clinical trial registered with www.controlled-trials.com (ISRCTN27114642) 05 December 2008.</abstract><cop>Australia</cop><pub>Ivyspring International Publisher</pub><pmid>28553169</pmid><doi>10.7150/ijms.18288</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1449-1907 |
| ispartof | International journal of medical sciences, 2017-01, Vol.14 (4), p.367-375 |
| issn | 1449-1907 1449-1907 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5436479 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Adult Esophagus - immunology Esophagus - pathology Esophagus - surgery Female Flow Cytometry Granulocyte-Macrophage Colony-Stimulating Factor - administration & dosage Granulocyte-Macrophage Colony-Stimulating Factor - immunology HLA-DR Antigens - immunology Humans Interferon-gamma - immunology Lipopolysaccharides - administration & dosage Male Monocytes - drug effects Monocytes - immunology Pancreas - immunology Pancreas - pathology Pancreas - surgery Postoperative Complications - drug therapy Postoperative Complications - genetics Postoperative Complications - physiopathology Research Paper T-Lymphocyte Subsets - immunology T-Lymphocytes, Regulatory - drug effects T-Lymphocytes, Regulatory - immunology Th17 Cells - drug effects Th17 Cells - immunology Tumor Necrosis Factor-alpha - immunology |
| title | In vivo application of Granulocyte-Macrophage Colony-stimulating Factor enhances postoperative qualitative monocytic function |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T18%3A47%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vivo%20application%20of%20Granulocyte-Macrophage%20Colony-stimulating%20Factor%20enhances%20postoperative%20qualitative%20monocytic%20function&rft.jtitle=International%20journal%20of%20medical%20sciences&rft.au=Lachmann,%20Gunnar&rft.date=2017-01-01&rft.volume=14&rft.issue=4&rft.spage=367&rft.epage=375&rft.pages=367-375&rft.issn=1449-1907&rft.eissn=1449-1907&rft_id=info:doi/10.7150/ijms.18288&rft_dat=%3Cproquest_pubme%3E1903439794%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1903439794&rft_id=info:pmid/28553169&rfr_iscdi=true |