Development and Characterization of EST-SSR Markers for Begonia luzhaiensis (Begoniaceae)
Premise of the study: Microsatellite primers were developed for Begonia luzhaiensis (Begoniaceae) to assess genetic diversity and population genetic structure. Methods and Results: Based on the transcriptome data of B. luzhaiensis, 60 primer pairs were selected for initial validation, of which 16 yi...
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| Veröffentlicht in: | Applications in plant sciences 2017-05, Vol.5 (5), p.n/a |
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| creator | Tseng, Yu-Hsin Huang, Han-Yau Xu, Wei-Bin Yang, Hsun-An Liu, Yan Peng, Ching-I Chung, Kuo-Fang |
| description | Premise of the study: Microsatellite primers were developed for Begonia luzhaiensis (Begoniaceae) to assess genetic diversity and population genetic structure. Methods and Results: Based on the transcriptome data of B. luzhaiensis, 60 primer pairs were selected for initial validation, of which 16 yielded polymorphic microsatellite loci in 57 individuals. The number of alleles observed for these 16 loci ranged from one to nine. The observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.000 to 0.804 with averages of 0.370 and 0.404, respectively. Five loci could be successfully amplified in B. leprosa. Conclusions: The expressed sequence tag–simple sequence repeat markers are the first specifically developed for B. luzhaiensis and the first developed in Begonia sect. Coelocentrum. These markers will be useful for future studies of the genetic structure and phylogeography of B. luzhaiensis. |
| doi_str_mv | 10.3732/apps.1700024 |
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Methods and Results: Based on the transcriptome data of B. luzhaiensis, 60 primer pairs were selected for initial validation, of which 16 yielded polymorphic microsatellite loci in 57 individuals. The number of alleles observed for these 16 loci ranged from one to nine. The observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.000 to 0.804 with averages of 0.370 and 0.404, respectively. Five loci could be successfully amplified in B. leprosa. Conclusions: The expressed sequence tag–simple sequence repeat markers are the first specifically developed for B. luzhaiensis and the first developed in Begonia sect. Coelocentrum. These markers will be useful for future studies of the genetic structure and phylogeography of B. luzhaiensis.</description><identifier>ISSN: 2168-0450</identifier><identifier>EISSN: 2168-0450</identifier><identifier>DOI: 10.3732/apps.1700024</identifier><identifier>PMID: 28529834</identifier><language>eng</language><publisher>United States: Botanical Society of America</publisher><subject>alleles ; Archives & records ; Begonia ; Begonia leprosa ; Begonia luzhaiensis ; Begoniaceae ; Biodiversity ; Expressed sequence tags ; expressed sequence tag–simple sequence repeat (EST-SSR) ; Gene expression ; Genetic diversity ; Genetic structure ; genetic variation ; Heterozygosity ; loci ; microsatellite repeats ; phylogeography ; Plant sciences ; Population ; Population genetics ; Primer Note ; PRIMER NOTES ; Primers ; section Coelocentrum ; transcriptome</subject><ispartof>Applications in plant sciences, 2017-05, Vol.5 (5), p.n/a</ispartof><rights>2017 The Author(s)</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by-nc-sa/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Tseng et al. Published by the Botanical Society of America 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b5264-69efa744b5d8d767771cc4173a333a182095dd74c49ee5caccaeb9aae85519c3</citedby><cites>FETCH-LOGICAL-b5264-69efa744b5d8d767771cc4173a333a182095dd74c49ee5caccaeb9aae85519c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.3732/apps.1700024$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435407/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>109,230,314,723,776,780,860,881,1411,11542,27903,27904,45553,45554,46030,46454,52697,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28529834$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tseng, Yu-Hsin</creatorcontrib><creatorcontrib>Huang, Han-Yau</creatorcontrib><creatorcontrib>Xu, Wei-Bin</creatorcontrib><creatorcontrib>Yang, Hsun-An</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Peng, Ching-I</creatorcontrib><creatorcontrib>Chung, Kuo-Fang</creatorcontrib><title>Development and Characterization of EST-SSR Markers for Begonia luzhaiensis (Begoniaceae)</title><title>Applications in plant sciences</title><addtitle>Appl Plant Sci</addtitle><description>Premise of the study: Microsatellite primers were developed for Begonia luzhaiensis (Begoniaceae) to assess genetic diversity and population genetic structure. Methods and Results: Based on the transcriptome data of B. luzhaiensis, 60 primer pairs were selected for initial validation, of which 16 yielded polymorphic microsatellite loci in 57 individuals. The number of alleles observed for these 16 loci ranged from one to nine. The observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.000 to 0.804 with averages of 0.370 and 0.404, respectively. Five loci could be successfully amplified in B. leprosa. Conclusions: The expressed sequence tag–simple sequence repeat markers are the first specifically developed for B. luzhaiensis and the first developed in Begonia sect. Coelocentrum. These markers will be useful for future studies of the genetic structure and phylogeography of B. luzhaiensis.</description><subject>alleles</subject><subject>Archives & records</subject><subject>Begonia</subject><subject>Begonia leprosa</subject><subject>Begonia luzhaiensis</subject><subject>Begoniaceae</subject><subject>Biodiversity</subject><subject>Expressed sequence tags</subject><subject>expressed sequence tag–simple sequence repeat (EST-SSR)</subject><subject>Gene expression</subject><subject>Genetic diversity</subject><subject>Genetic structure</subject><subject>genetic variation</subject><subject>Heterozygosity</subject><subject>loci</subject><subject>microsatellite repeats</subject><subject>phylogeography</subject><subject>Plant sciences</subject><subject>Population</subject><subject>Population genetics</subject><subject>Primer Note</subject><subject>PRIMER NOTES</subject><subject>Primers</subject><subject>section Coelocentrum</subject><subject>transcriptome</subject><issn>2168-0450</issn><issn>2168-0450</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1v1DAQhiMEolXpjTOKxKUgUvwZx5dKZVs-pCIQuxdO1sSZdF2ydrA3rdpfj6tdSuFQJEtjzTzzambeonhOySFXnL2FcUyHVBFCmHhU7DJaNxURkjy-998p9lO6yAhpckqLp8UOayTTDRe7xfcTvMQhjCv06xJ8V86WEMGuMbobWLvgy9CXp_NFNZ9_Kz9D_IExlX2I5Ts8D95BOUw3S3Dok0vlwTZpEfDVs-JJD0PC_W3cKxbvTxezj9XZlw-fZsdnVStZLapaYw9KiFZ2TadqpRS1VlDFgXMOtGFEy65TwgqNKC1YC9hqAGykpNryveJoIztO7Qo7m_eIMJgxuhXEaxPAmb8r3i3Nebg0UnApiMoCB1uBGH5OmNZm5ZLFYQCPYUqG5cNxLYRo_otSTSinhDU8oy__QS_CFH0-hGFME1bX-WXqzYayMaQUsb-bmxJza7C5NdhsDc74i_u73sG_7cyA3ABXbsDrB8XM8dc5_yP8etPXuhA8PjzFL3kWvpg</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Tseng, Yu-Hsin</creator><creator>Huang, Han-Yau</creator><creator>Xu, Wei-Bin</creator><creator>Yang, Hsun-An</creator><creator>Liu, Yan</creator><creator>Peng, Ching-I</creator><creator>Chung, Kuo-Fang</creator><general>Botanical Society of America</general><general>John Wiley & Sons, Inc</general><scope>24P</scope><scope>WIN</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>201705</creationdate><title>Development and Characterization of EST-SSR Markers for Begonia luzhaiensis (Begoniaceae)</title><author>Tseng, Yu-Hsin ; Huang, Han-Yau ; Xu, Wei-Bin ; Yang, Hsun-An ; Liu, Yan ; Peng, Ching-I ; Chung, Kuo-Fang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b5264-69efa744b5d8d767771cc4173a333a182095dd74c49ee5caccaeb9aae85519c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>alleles</topic><topic>Archives & records</topic><topic>Begonia</topic><topic>Begonia leprosa</topic><topic>Begonia luzhaiensis</topic><topic>Begoniaceae</topic><topic>Biodiversity</topic><topic>Expressed sequence tags</topic><topic>expressed sequence tag–simple sequence repeat (EST-SSR)</topic><topic>Gene expression</topic><topic>Genetic diversity</topic><topic>Genetic structure</topic><topic>genetic variation</topic><topic>Heterozygosity</topic><topic>loci</topic><topic>microsatellite repeats</topic><topic>phylogeography</topic><topic>Plant sciences</topic><topic>Population</topic><topic>Population genetics</topic><topic>Primer Note</topic><topic>PRIMER NOTES</topic><topic>Primers</topic><topic>section Coelocentrum</topic><topic>transcriptome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tseng, Yu-Hsin</creatorcontrib><creatorcontrib>Huang, Han-Yau</creatorcontrib><creatorcontrib>Xu, Wei-Bin</creatorcontrib><creatorcontrib>Yang, Hsun-An</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Peng, Ching-I</creatorcontrib><creatorcontrib>Chung, Kuo-Fang</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applications in plant sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tseng, Yu-Hsin</au><au>Huang, Han-Yau</au><au>Xu, Wei-Bin</au><au>Yang, Hsun-An</au><au>Liu, Yan</au><au>Peng, Ching-I</au><au>Chung, Kuo-Fang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Characterization of EST-SSR Markers for Begonia luzhaiensis (Begoniaceae)</atitle><jtitle>Applications in plant sciences</jtitle><addtitle>Appl Plant Sci</addtitle><date>2017-05</date><risdate>2017</risdate><volume>5</volume><issue>5</issue><epage>n/a</epage><issn>2168-0450</issn><eissn>2168-0450</eissn><abstract>Premise of the study: Microsatellite primers were developed for Begonia luzhaiensis (Begoniaceae) to assess genetic diversity and population genetic structure. Methods and Results: Based on the transcriptome data of B. luzhaiensis, 60 primer pairs were selected for initial validation, of which 16 yielded polymorphic microsatellite loci in 57 individuals. The number of alleles observed for these 16 loci ranged from one to nine. The observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.000 to 0.804 with averages of 0.370 and 0.404, respectively. Five loci could be successfully amplified in B. leprosa. Conclusions: The expressed sequence tag–simple sequence repeat markers are the first specifically developed for B. luzhaiensis and the first developed in Begonia sect. Coelocentrum. These markers will be useful for future studies of the genetic structure and phylogeography of B. luzhaiensis.</abstract><cop>United States</cop><pub>Botanical Society of America</pub><pmid>28529834</pmid><doi>10.3732/apps.1700024</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alleles Archives & records Begonia Begonia leprosa Begonia luzhaiensis Begoniaceae Biodiversity Expressed sequence tags expressed sequence tag–simple sequence repeat (EST-SSR) Gene expression Genetic diversity Genetic structure genetic variation Heterozygosity loci microsatellite repeats phylogeography Plant sciences Population Population genetics Primer Note PRIMER NOTES Primers section Coelocentrum transcriptome |
| title | Development and Characterization of EST-SSR Markers for Begonia luzhaiensis (Begoniaceae) |
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