Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb
Background Peucedanum japonicum Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration s...
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| Veröffentlicht in: | Botanical studies 2016-03, Vol.57 (1), p.9-9, Article 9 |
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| creator | Chen, Chia-Chen Agrawal, Dinesh Chandra Lee, Maw-Rong Lee, Ren-Jye Kuo, Chao-Lin Wu, Chi-Rei Tsay, Hsin-Sheng Chang, Hung-Chi |
| description | Background
Peucedanum japonicum
Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration system of
P. japonicum
via somatic embryogenesis and to analyse chlorogenic acid and rutin contents in a few commercially available plant products of
P. japonicum
in Japan and Taiwan markets, and tissue culture plants derived from somatic embryos.
Results
Induction of somatic embryogenesis could be achieved when root derived calli after three subcultures were transferred from Murashige Skoog’s salts and vitamins (MS basal) medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.1–5 mg/L) to a medium with abscisic acid (ABA) (0.5–4 mg/L), or exposed to eight different light spectra provided by light-emitting diode (LED) sources. Concentrations of ABA and LED light spectra had an influence on number of somatic embryos induced and proliferation of callus. Development of secondary somatic embryos and conversion of embryos to plantlets was achieved on a medium with ABA, or their exposure to red or blue lights in a special incubation chamber. Four months old tissue culture plants derived from somatic embryos showed significantly higher levels of chlorogenic acid (10.5 mg/g dw) compared to commercial product sold in Japanese market (0.55 mg/g dw). However, rutin was absent in tissue culture plants in contrast to commercial sample (0.33 mg/g dw).
Conclusion
In this report, we describe in vitro plant regeneration system in
P. japonicum
via somatic embryogenesis and production of chlorogenic acid in tissue culture plants. The present study has application in further tissue culture propagation of elite plant material with high chlorogenic acid content, and identification of high yielding plants with the LC–MS method. |
| doi_str_mv | 10.1186/s40529-016-0124-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5430566</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4312229961</sourcerecordid><originalsourceid>FETCH-LOGICAL-c569t-e9f888f0b924bfd429b9a52c623669712bbc81e90849f39f5674395b68325ef43</originalsourceid><addsrcrecordid>eNqFktGK1DAUhoso7rr6AN5IwBtvuiZpkkm8EGRcdWFEwRW8C0maTDO0yZi0C7NXvoOv4JP5JKYz67IK4kXpofnOl9PDX1WPETxFiLPnmUCKRQ0RKw8m9dWd6hgJIeoGIXi31BwtagLZl6PqQc4bCBnCDb5fHWFOxYIgflz9OA-un2wwFkQHVmevQe_X3Qjy1poxKRAD8AFc-jFFkOOgRm-AHXTaxbUNNvsMVGjBavnz2_f3n0qt-t38sbhM18c0U6VDGd_uwTSNfm_8aCdjWxWmAWzUNhaoVBfdFPTpC6DAYFtvfLGBzib9sLrnVJ_to-v3SfX5zdnF8l29-vD2fPlqVRvKxFhb4TjnDmqBiXYtwUILRbFhuGFMLBDW2nBkBeREuEY4yhakEVQz3mBqHWlOqpcH73bSZQJjQ1lBL7fJDyrtZFRe_nkSfCfX8VJS0kDKWBE8uxak-HWyeZSDz8b2vQo2TlliCGFT9i7of1E0j9lAiHFBn_6FbuKUynIKxRktUrIXogNlUsw5WXczN4Jyjos8xEWWuMg5LvKq9Dy5_cM3Hb_zUQB8AHI5Cmubbl39T-sv_JPNnw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1865200495</pqid></control><display><type>article</type><title>Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><source>PubMed Central Open Access</source><source>Springer Nature OA Free Journals</source><creator>Chen, Chia-Chen ; Agrawal, Dinesh Chandra ; Lee, Maw-Rong ; Lee, Ren-Jye ; Kuo, Chao-Lin ; Wu, Chi-Rei ; Tsay, Hsin-Sheng ; Chang, Hung-Chi</creator><creatorcontrib>Chen, Chia-Chen ; Agrawal, Dinesh Chandra ; Lee, Maw-Rong ; Lee, Ren-Jye ; Kuo, Chao-Lin ; Wu, Chi-Rei ; Tsay, Hsin-Sheng ; Chang, Hung-Chi</creatorcontrib><description>Background
Peucedanum japonicum
Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration system of
P. japonicum
via somatic embryogenesis and to analyse chlorogenic acid and rutin contents in a few commercially available plant products of
P. japonicum
in Japan and Taiwan markets, and tissue culture plants derived from somatic embryos.
Results
Induction of somatic embryogenesis could be achieved when root derived calli after three subcultures were transferred from Murashige Skoog’s salts and vitamins (MS basal) medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.1–5 mg/L) to a medium with abscisic acid (ABA) (0.5–4 mg/L), or exposed to eight different light spectra provided by light-emitting diode (LED) sources. Concentrations of ABA and LED light spectra had an influence on number of somatic embryos induced and proliferation of callus. Development of secondary somatic embryos and conversion of embryos to plantlets was achieved on a medium with ABA, or their exposure to red or blue lights in a special incubation chamber. Four months old tissue culture plants derived from somatic embryos showed significantly higher levels of chlorogenic acid (10.5 mg/g dw) compared to commercial product sold in Japanese market (0.55 mg/g dw). However, rutin was absent in tissue culture plants in contrast to commercial sample (0.33 mg/g dw).
Conclusion
In this report, we describe in vitro plant regeneration system in
P. japonicum
via somatic embryogenesis and production of chlorogenic acid in tissue culture plants. The present study has application in further tissue culture propagation of elite plant material with high chlorogenic acid content, and identification of high yielding plants with the LC–MS method.</description><identifier>ISSN: 1817-406X</identifier><identifier>ISSN: 1999-3110</identifier><identifier>EISSN: 1999-3110</identifier><identifier>DOI: 10.1186/s40529-016-0124-z</identifier><identifier>PMID: 28597418</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>2,4-D ; abscisic acid ; antioxidants ; Biomedical and Life Sciences ; blue light ; callus ; chlorogenic acid ; Ecology ; Japan ; Life Sciences ; liquid chromatography ; markets ; mass spectrometry ; medicinal plants ; medicinal properties ; Original ; Original Article ; Peucedanum japonicum ; Physiology ; Plant Genetics and Genomics ; plant products ; Plant Sciences ; plantlets ; rutin ; salts ; somatic embryogenesis ; somatic embryos ; Taiwan ; tissue culture ; vitamins</subject><ispartof>Botanical studies, 2016-03, Vol.57 (1), p.9-9, Article 9</ispartof><rights>Chen et al. 2016</rights><rights>Botanical Studies is a copyright of Springer, 2016.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c569t-e9f888f0b924bfd429b9a52c623669712bbc81e90849f39f5674395b68325ef43</citedby><cites>FETCH-LOGICAL-c569t-e9f888f0b924bfd429b9a52c623669712bbc81e90849f39f5674395b68325ef43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430566/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430566/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,41464,42165,42533,51294,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28597418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Chia-Chen</creatorcontrib><creatorcontrib>Agrawal, Dinesh Chandra</creatorcontrib><creatorcontrib>Lee, Maw-Rong</creatorcontrib><creatorcontrib>Lee, Ren-Jye</creatorcontrib><creatorcontrib>Kuo, Chao-Lin</creatorcontrib><creatorcontrib>Wu, Chi-Rei</creatorcontrib><creatorcontrib>Tsay, Hsin-Sheng</creatorcontrib><creatorcontrib>Chang, Hung-Chi</creatorcontrib><title>Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb</title><title>Botanical studies</title><addtitle>Bot Stud</addtitle><addtitle>Bot Stud</addtitle><description>Background
Peucedanum japonicum
Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration system of
P. japonicum
via somatic embryogenesis and to analyse chlorogenic acid and rutin contents in a few commercially available plant products of
P. japonicum
in Japan and Taiwan markets, and tissue culture plants derived from somatic embryos.
Results
Induction of somatic embryogenesis could be achieved when root derived calli after three subcultures were transferred from Murashige Skoog’s salts and vitamins (MS basal) medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.1–5 mg/L) to a medium with abscisic acid (ABA) (0.5–4 mg/L), or exposed to eight different light spectra provided by light-emitting diode (LED) sources. Concentrations of ABA and LED light spectra had an influence on number of somatic embryos induced and proliferation of callus. Development of secondary somatic embryos and conversion of embryos to plantlets was achieved on a medium with ABA, or their exposure to red or blue lights in a special incubation chamber. Four months old tissue culture plants derived from somatic embryos showed significantly higher levels of chlorogenic acid (10.5 mg/g dw) compared to commercial product sold in Japanese market (0.55 mg/g dw). However, rutin was absent in tissue culture plants in contrast to commercial sample (0.33 mg/g dw).
Conclusion
In this report, we describe in vitro plant regeneration system in
P. japonicum
via somatic embryogenesis and production of chlorogenic acid in tissue culture plants. The present study has application in further tissue culture propagation of elite plant material with high chlorogenic acid content, and identification of high yielding plants with the LC–MS method.</description><subject>2,4-D</subject><subject>abscisic acid</subject><subject>antioxidants</subject><subject>Biomedical and Life Sciences</subject><subject>blue light</subject><subject>callus</subject><subject>chlorogenic acid</subject><subject>Ecology</subject><subject>Japan</subject><subject>Life Sciences</subject><subject>liquid chromatography</subject><subject>markets</subject><subject>mass spectrometry</subject><subject>medicinal plants</subject><subject>medicinal properties</subject><subject>Original</subject><subject>Original Article</subject><subject>Peucedanum japonicum</subject><subject>Physiology</subject><subject>Plant Genetics and Genomics</subject><subject>plant products</subject><subject>Plant Sciences</subject><subject>plantlets</subject><subject>rutin</subject><subject>salts</subject><subject>somatic embryogenesis</subject><subject>somatic embryos</subject><subject>Taiwan</subject><subject>tissue culture</subject><subject>vitamins</subject><issn>1817-406X</issn><issn>1999-3110</issn><issn>1999-3110</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNqFktGK1DAUhoso7rr6AN5IwBtvuiZpkkm8EGRcdWFEwRW8C0maTDO0yZi0C7NXvoOv4JP5JKYz67IK4kXpofnOl9PDX1WPETxFiLPnmUCKRQ0RKw8m9dWd6hgJIeoGIXi31BwtagLZl6PqQc4bCBnCDb5fHWFOxYIgflz9OA-un2wwFkQHVmevQe_X3Qjy1poxKRAD8AFc-jFFkOOgRm-AHXTaxbUNNvsMVGjBavnz2_f3n0qt-t38sbhM18c0U6VDGd_uwTSNfm_8aCdjWxWmAWzUNhaoVBfdFPTpC6DAYFtvfLGBzib9sLrnVJ_to-v3SfX5zdnF8l29-vD2fPlqVRvKxFhb4TjnDmqBiXYtwUILRbFhuGFMLBDW2nBkBeREuEY4yhakEVQz3mBqHWlOqpcH73bSZQJjQ1lBL7fJDyrtZFRe_nkSfCfX8VJS0kDKWBE8uxak-HWyeZSDz8b2vQo2TlliCGFT9i7of1E0j9lAiHFBn_6FbuKUynIKxRktUrIXogNlUsw5WXczN4Jyjos8xEWWuMg5LvKq9Dy5_cM3Hb_zUQB8AHI5Cmubbl39T-sv_JPNnw</recordid><startdate>20160310</startdate><enddate>20160310</enddate><creator>Chen, Chia-Chen</creator><creator>Agrawal, Dinesh Chandra</creator><creator>Lee, Maw-Rong</creator><creator>Lee, Ren-Jye</creator><creator>Kuo, Chao-Lin</creator><creator>Wu, Chi-Rei</creator><creator>Tsay, Hsin-Sheng</creator><creator>Chang, Hung-Chi</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20160310</creationdate><title>Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb</title><author>Chen, Chia-Chen ; Agrawal, Dinesh Chandra ; Lee, Maw-Rong ; Lee, Ren-Jye ; Kuo, Chao-Lin ; Wu, Chi-Rei ; Tsay, Hsin-Sheng ; Chang, Hung-Chi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c569t-e9f888f0b924bfd429b9a52c623669712bbc81e90849f39f5674395b68325ef43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>2,4-D</topic><topic>abscisic acid</topic><topic>antioxidants</topic><topic>Biomedical and Life Sciences</topic><topic>blue light</topic><topic>callus</topic><topic>chlorogenic acid</topic><topic>Ecology</topic><topic>Japan</topic><topic>Life Sciences</topic><topic>liquid chromatography</topic><topic>markets</topic><topic>mass spectrometry</topic><topic>medicinal plants</topic><topic>medicinal properties</topic><topic>Original</topic><topic>Original Article</topic><topic>Peucedanum japonicum</topic><topic>Physiology</topic><topic>Plant Genetics and Genomics</topic><topic>plant products</topic><topic>Plant Sciences</topic><topic>plantlets</topic><topic>rutin</topic><topic>salts</topic><topic>somatic embryogenesis</topic><topic>somatic embryos</topic><topic>Taiwan</topic><topic>tissue culture</topic><topic>vitamins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Chia-Chen</creatorcontrib><creatorcontrib>Agrawal, Dinesh Chandra</creatorcontrib><creatorcontrib>Lee, Maw-Rong</creatorcontrib><creatorcontrib>Lee, Ren-Jye</creatorcontrib><creatorcontrib>Kuo, Chao-Lin</creatorcontrib><creatorcontrib>Wu, Chi-Rei</creatorcontrib><creatorcontrib>Tsay, Hsin-Sheng</creatorcontrib><creatorcontrib>Chang, Hung-Chi</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Botanical studies</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Chia-Chen</au><au>Agrawal, Dinesh Chandra</au><au>Lee, Maw-Rong</au><au>Lee, Ren-Jye</au><au>Kuo, Chao-Lin</au><au>Wu, Chi-Rei</au><au>Tsay, Hsin-Sheng</au><au>Chang, Hung-Chi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb</atitle><jtitle>Botanical studies</jtitle><stitle>Bot Stud</stitle><addtitle>Bot Stud</addtitle><date>2016-03-10</date><risdate>2016</risdate><volume>57</volume><issue>1</issue><spage>9</spage><epage>9</epage><pages>9-9</pages><artnum>9</artnum><issn>1817-406X</issn><issn>1999-3110</issn><eissn>1999-3110</eissn><abstract>Background
Peucedanum japonicum
Thunb, an important medicinal herb is reported to possess pharmacological properties such as anti-obesity, anti-oxidant, anti-inflammatory, anti-bacterial, anti-diabetic and anti-platelet aggregation. The present study aimed to develop an in vitro plant regeneration system of
P. japonicum
via somatic embryogenesis and to analyse chlorogenic acid and rutin contents in a few commercially available plant products of
P. japonicum
in Japan and Taiwan markets, and tissue culture plants derived from somatic embryos.
Results
Induction of somatic embryogenesis could be achieved when root derived calli after three subcultures were transferred from Murashige Skoog’s salts and vitamins (MS basal) medium with 2,4-dichlorophenoxyacetic acid (2,4-D) (0.1–5 mg/L) to a medium with abscisic acid (ABA) (0.5–4 mg/L), or exposed to eight different light spectra provided by light-emitting diode (LED) sources. Concentrations of ABA and LED light spectra had an influence on number of somatic embryos induced and proliferation of callus. Development of secondary somatic embryos and conversion of embryos to plantlets was achieved on a medium with ABA, or their exposure to red or blue lights in a special incubation chamber. Four months old tissue culture plants derived from somatic embryos showed significantly higher levels of chlorogenic acid (10.5 mg/g dw) compared to commercial product sold in Japanese market (0.55 mg/g dw). However, rutin was absent in tissue culture plants in contrast to commercial sample (0.33 mg/g dw).
Conclusion
In this report, we describe in vitro plant regeneration system in
P. japonicum
via somatic embryogenesis and production of chlorogenic acid in tissue culture plants. The present study has application in further tissue culture propagation of elite plant material with high chlorogenic acid content, and identification of high yielding plants with the LC–MS method.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28597418</pmid><doi>10.1186/s40529-016-0124-z</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 1817-406X 1999-3110 1999-3110 |
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| subjects | 2,4-D abscisic acid antioxidants Biomedical and Life Sciences blue light callus chlorogenic acid Ecology Japan Life Sciences liquid chromatography markets mass spectrometry medicinal plants medicinal properties Original Original Article Peucedanum japonicum Physiology Plant Genetics and Genomics plant products Plant Sciences plantlets rutin salts somatic embryogenesis somatic embryos Taiwan tissue culture vitamins |
| title | Influence of LED light spectra on in vitro somatic embryogenesis and LC–MS analysis of chlorogenic acid and rutin in Peucedanum japonicum Thunb.: a medicinal herb |
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