Indole-3-acetic acid oxidase from peas. I. Occurrence and distribution of peroxidative and nonperoxidative forms

Tissues of etiolated pea seedlings variety Alaska were examined for the presence of peroxidative and nonperoxidative forms of indoleacetic acid (IAA) oxidase. Enzymes were extracted in a sequence involving acetone powder preparation from pea tissues, buffer extraction of the powder, ammonium sulfate...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology (Bethesda) 1979-04, Vol.63 (4), p.696-699
Hauptverfasser:	Bryant, S D, Lane, F E
Format:	Artikel
Sprache:	eng
Schlagworte:	Electrophoresis Enzymes Gels Indoleacetic acids Insulin antibodies Internodes Oxidases Peas Plants Seedlings
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	699
container_issue	4
container_start_page	696
container_title	Plant physiology (Bethesda)
container_volume	63
creator	Bryant, S D Lane, F E
description	Tissues of etiolated pea seedlings variety Alaska were examined for the presence of peroxidative and nonperoxidative forms of indoleacetic acid (IAA) oxidase. Enzymes were extracted in a sequence involving acetone powder preparation from pea tissues, buffer extraction of the powder, ammonium sulfate precipitation, dialysis, lyophilization, and acrylamide gel electrophoresis. Electrophoretically separable proteins were assayed for IAA oxidase activity with the Salkowski test, and peroxidase activity was based on the color reaction with benzidine and H2O2. Each tissue examined contained several nonperoxidative IAA oxidases. No tissue contained more than three peroxidative IAA oxidases, whereas the plumule hooks (a tissue with a high IAA oxidase activity) contained no detectable peroxidases. The results indicate that nonperoxidative IAA oxidases might play a major role in the regulation of IAA content in pea seedlings.
doi_str_mv	10.1104/pp.63.4.696
format	Article
fullrecord	<record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_542899</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4265726</jstor_id><sourcerecordid>4265726</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2249-2bb9a770c0a0a74b4a3888418384a4462f569486791e149358d8d0155e10de43</originalsourceid><addsrcrecordid>eNpVkc1v1DAQxS0EokvhxBUh3zhUCf4YO_aBQ1VRWKlSD5Sz5dhOcZXYwU4q-O8J7Kq0pxnN-72ZkR5CbylpKSXwcZ5byVtopZbP0I4KzhomQD1HO0K2niilT9CrWu8IIZRTeIlOqJSSdBp2aN4nn8fQ8Ma6sESHrYse51_R2xrwUPKE52Bri_ctvnZuLSUkF7BNHvtYlxL7dYk54TxsXPnnW-L9AUg5PZkNuUz1NXox2LGGN8d6im4uP99cfG2urr_sL86vGscY6Ib1vbZdRxyxxHbQg-VKKaCKK7AAkg1CalCy0zRQ0FworzyhQgRKfAB-ij4d1s5rPwXvQlqKHc1c4mTLb5NtNE-VFH-Y23xvBDCl9eb_cPSX_HMNdTFTrC6Mo00hr9V0nINSkrKNPDuQruRaSxgejlBi_gZk5tlIbsBsAW30-8d__WePiWzAuwNwV5dcHnRgUnTskX-w2djbEqv5_o1qRYggIJXgfwDfX58f</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733488612</pqid></control><display><type>article</type><title>Indole-3-acetic acid oxidase from peas. I. Occurrence and distribution of peroxidative and nonperoxidative forms</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>JSTOR Archive Collection A-Z Listing</source><source>Alma/SFX Local Collection</source><creator>Bryant, S D ; Lane, F E</creator><creatorcontrib>Bryant, S D ; Lane, F E ; Padua Univ. (Italy). Istituto di Pomologia</creatorcontrib><description>Tissues of etiolated pea seedlings variety Alaska were examined for the presence of peroxidative and nonperoxidative forms of indoleacetic acid (IAA) oxidase. Enzymes were extracted in a sequence involving acetone powder preparation from pea tissues, buffer extraction of the powder, ammonium sulfate precipitation, dialysis, lyophilization, and acrylamide gel electrophoresis. Electrophoretically separable proteins were assayed for IAA oxidase activity with the Salkowski test, and peroxidase activity was based on the color reaction with benzidine and H2O2. Each tissue examined contained several nonperoxidative IAA oxidases. No tissue contained more than three peroxidative IAA oxidases, whereas the plumule hooks (a tissue with a high IAA oxidase activity) contained no detectable peroxidases. The results indicate that nonperoxidative IAA oxidases might play a major role in the regulation of IAA content in pea seedlings.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.63.4.696</identifier><identifier>PMID: 16660794</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>Electrophoresis ; Enzymes ; Gels ; Indoleacetic acids ; Insulin antibodies ; Internodes ; Oxidases ; Peas ; Plants ; Seedlings</subject><ispartof>Plant physiology (Bethesda), 1979-04, Vol.63 (4), p.696-699</ispartof><rights>Copyright 1979 The American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4265726$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4265726$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16660794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bryant, S D</creatorcontrib><creatorcontrib>Lane, F E</creatorcontrib><creatorcontrib>Padua Univ. (Italy). Istituto di Pomologia</creatorcontrib><title>Indole-3-acetic acid oxidase from peas. I. Occurrence and distribution of peroxidative and nonperoxidative forms</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Tissues of etiolated pea seedlings variety Alaska were examined for the presence of peroxidative and nonperoxidative forms of indoleacetic acid (IAA) oxidase. Enzymes were extracted in a sequence involving acetone powder preparation from pea tissues, buffer extraction of the powder, ammonium sulfate precipitation, dialysis, lyophilization, and acrylamide gel electrophoresis. Electrophoretically separable proteins were assayed for IAA oxidase activity with the Salkowski test, and peroxidase activity was based on the color reaction with benzidine and H2O2. Each tissue examined contained several nonperoxidative IAA oxidases. No tissue contained more than three peroxidative IAA oxidases, whereas the plumule hooks (a tissue with a high IAA oxidase activity) contained no detectable peroxidases. The results indicate that nonperoxidative IAA oxidases might play a major role in the regulation of IAA content in pea seedlings.</description><subject>Electrophoresis</subject><subject>Enzymes</subject><subject>Gels</subject><subject>Indoleacetic acids</subject><subject>Insulin antibodies</subject><subject>Internodes</subject><subject>Oxidases</subject><subject>Peas</subject><subject>Plants</subject><subject>Seedlings</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1979</creationdate><recordtype>article</recordtype><recordid>eNpVkc1v1DAQxS0EokvhxBUh3zhUCf4YO_aBQ1VRWKlSD5Sz5dhOcZXYwU4q-O8J7Kq0pxnN-72ZkR5CbylpKSXwcZ5byVtopZbP0I4KzhomQD1HO0K2niilT9CrWu8IIZRTeIlOqJSSdBp2aN4nn8fQ8Ma6sESHrYse51_R2xrwUPKE52Bri_ctvnZuLSUkF7BNHvtYlxL7dYk54TxsXPnnW-L9AUg5PZkNuUz1NXox2LGGN8d6im4uP99cfG2urr_sL86vGscY6Ib1vbZdRxyxxHbQg-VKKaCKK7AAkg1CalCy0zRQ0FworzyhQgRKfAB-ij4d1s5rPwXvQlqKHc1c4mTLb5NtNE-VFH-Y23xvBDCl9eb_cPSX_HMNdTFTrC6Mo00hr9V0nINSkrKNPDuQruRaSxgejlBi_gZk5tlIbsBsAW30-8d__WePiWzAuwNwV5dcHnRgUnTskX-w2djbEqv5_o1qRYggIJXgfwDfX58f</recordid><startdate>19790401</startdate><enddate>19790401</enddate><creator>Bryant, S D</creator><creator>Lane, F E</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19790401</creationdate><title>Indole-3-acetic acid oxidase from peas. I. Occurrence and distribution of peroxidative and nonperoxidative forms</title><author>Bryant, S D ; Lane, F E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2249-2bb9a770c0a0a74b4a3888418384a4462f569486791e149358d8d0155e10de43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1979</creationdate><topic>Electrophoresis</topic><topic>Enzymes</topic><topic>Gels</topic><topic>Indoleacetic acids</topic><topic>Insulin antibodies</topic><topic>Internodes</topic><topic>Oxidases</topic><topic>Peas</topic><topic>Plants</topic><topic>Seedlings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bryant, S D</creatorcontrib><creatorcontrib>Lane, F E</creatorcontrib><creatorcontrib>Padua Univ. (Italy). Istituto di Pomologia</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bryant, S D</au><au>Lane, F E</au><aucorp>Padua Univ. (Italy). Istituto di Pomologia</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Indole-3-acetic acid oxidase from peas. I. Occurrence and distribution of peroxidative and nonperoxidative forms</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1979-04-01</date><risdate>1979</risdate><volume>63</volume><issue>4</issue><spage>696</spage><epage>699</epage><pages>696-699</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>Tissues of etiolated pea seedlings variety Alaska were examined for the presence of peroxidative and nonperoxidative forms of indoleacetic acid (IAA) oxidase. Enzymes were extracted in a sequence involving acetone powder preparation from pea tissues, buffer extraction of the powder, ammonium sulfate precipitation, dialysis, lyophilization, and acrylamide gel electrophoresis. Electrophoretically separable proteins were assayed for IAA oxidase activity with the Salkowski test, and peroxidase activity was based on the color reaction with benzidine and H2O2. Each tissue examined contained several nonperoxidative IAA oxidases. No tissue contained more than three peroxidative IAA oxidases, whereas the plumule hooks (a tissue with a high IAA oxidase activity) contained no detectable peroxidases. The results indicate that nonperoxidative IAA oxidases might play a major role in the regulation of IAA content in pea seedlings.</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>16660794</pmid><doi>10.1104/pp.63.4.696</doi><tpages>4</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0032-0889
ispartof	Plant physiology (Bethesda), 1979-04, Vol.63 (4), p.696-699
issn	0032-0889 1532-2548
language	eng
recordid	cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_542899
source	Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Alma/SFX Local Collection
subjects	Electrophoresis Enzymes Gels Indoleacetic acids Insulin antibodies Internodes Oxidases Peas Plants Seedlings
title	Indole-3-acetic acid oxidase from peas. I. Occurrence and distribution of peroxidative and nonperoxidative forms
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T17%3A27%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Indole-3-acetic%20acid%20oxidase%20from%20peas.%20I.%20Occurrence%20and%20distribution%20of%20peroxidative%20and%20nonperoxidative%20forms&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Bryant,%20S%20D&rft.aucorp=Padua%20Univ.%20(Italy).%20Istituto%20di%20Pomologia&rft.date=1979-04-01&rft.volume=63&rft.issue=4&rft.spage=696&rft.epage=699&rft.pages=696-699&rft.issn=0032-0889&rft.eissn=1532-2548&rft_id=info:doi/10.1104/pp.63.4.696&rft_dat=%3Cjstor_pubme%3E4265726%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733488612&rft_id=info:pmid/16660794&rft_jstor_id=4265726&rfr_iscdi=true