ESBL Production Among E. coli and Klebsiella spp. Causing Urinary Tract Infection: A Hospital Based Study
Increase in extended-spectrum β-lactamases (ESBL) producing microbes in recent years has led to limitations of treatment options. This study aimed to assess the prevalence of ESBL producing and spp. at a tertiary hospital in Nepal. A total of 2209 non-repetitive mid-stream urine (MSU) samples were c...
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| Veröffentlicht in: | The open microbiology journal 2017-04, Vol.11 (1), p.23-30 |
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| creator | Shakya, Pooja Shrestha, Dhiraj Maharjan, Elina Sharma, Vijay K Paudyal, Rabin |
| description | Increase in extended-spectrum β-lactamases (ESBL) producing microbes in recent years has led to limitations of treatment options. This study aimed to assess the prevalence of ESBL producing
and
spp. at a tertiary hospital in Nepal.
A total of 2209 non-repetitive mid-stream urine (MSU) samples were collected during the study period (March to September 2014). Identification of the isolates was done by Gram's staining followed by biochemical tests. Antibiotic susceptibility testing was done by modified Kirby-Bauer disc diffusion method and interpretation was done following Clinical and Laboratory Standard Institute (CLSI) guidelines, 2013. ESBL screening among
and
spp. isolates were done using ceftriaxone, cefotaxime, ceftazidime and cefpodoxime. The confirmation was done by phenotypic disc diffusion test (combined disc method) using ceftazidime (30µg) and ceftazidime plus clavulanic acid (30/10µg), and cefotaxime (30µg) and cefotaxime plus clavulanic acid (30/10µg) disc as per CLSI guidelines.
A total of 451 samples showed significant bacteriuria with 365 (80.9%)
, 17 (3.8%)
and 3 (0.7%)
. Of 451 isolates, 236 (52.3%) were found MDR strains. By combined disk test, 33 (91.7%)
and 3 (8.3%)
spp. were found ESBL producers.
Higher prevalence of ESBL producing
and
spp. was observed warranting prompt need of surveillance for effective management of such MDR strains. |
| doi_str_mv | 10.2174/1874285801711010023 |
| format | Article |
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and
spp. at a tertiary hospital in Nepal.
A total of 2209 non-repetitive mid-stream urine (MSU) samples were collected during the study period (March to September 2014). Identification of the isolates was done by Gram's staining followed by biochemical tests. Antibiotic susceptibility testing was done by modified Kirby-Bauer disc diffusion method and interpretation was done following Clinical and Laboratory Standard Institute (CLSI) guidelines, 2013. ESBL screening among
and
spp. isolates were done using ceftriaxone, cefotaxime, ceftazidime and cefpodoxime. The confirmation was done by phenotypic disc diffusion test (combined disc method) using ceftazidime (30µg) and ceftazidime plus clavulanic acid (30/10µg), and cefotaxime (30µg) and cefotaxime plus clavulanic acid (30/10µg) disc as per CLSI guidelines.
A total of 451 samples showed significant bacteriuria with 365 (80.9%)
, 17 (3.8%)
and 3 (0.7%)
. Of 451 isolates, 236 (52.3%) were found MDR strains. By combined disk test, 33 (91.7%)
and 3 (8.3%)
spp. were found ESBL producers.
Higher prevalence of ESBL producing
and
spp. was observed warranting prompt need of surveillance for effective management of such MDR strains.</description><identifier>ISSN: 1874-2858</identifier><identifier>EISSN: 1874-2858</identifier><identifier>DOI: 10.2174/1874285801711010023</identifier><identifier>PMID: 28553414</identifier><language>eng</language><publisher>United Arab Emirates: Bentham Open</publisher><ispartof>The open microbiology journal, 2017-04, Vol.11 (1), p.23-30</ispartof><rights>2017 Shakya 2017 Bentham Open</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3863-486f5e7c159812631f26800334fe821285b6a6dd0d49548f68cc196e96004bd93</citedby><cites>FETCH-LOGICAL-c3863-486f5e7c159812631f26800334fe821285b6a6dd0d49548f68cc196e96004bd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427687/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5427687/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28553414$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shakya, Pooja</creatorcontrib><creatorcontrib>Shrestha, Dhiraj</creatorcontrib><creatorcontrib>Maharjan, Elina</creatorcontrib><creatorcontrib>Sharma, Vijay K</creatorcontrib><creatorcontrib>Paudyal, Rabin</creatorcontrib><title>ESBL Production Among E. coli and Klebsiella spp. Causing Urinary Tract Infection: A Hospital Based Study</title><title>The open microbiology journal</title><addtitle>Open Microbiol J</addtitle><description>Increase in extended-spectrum β-lactamases (ESBL) producing microbes in recent years has led to limitations of treatment options. This study aimed to assess the prevalence of ESBL producing
and
spp. at a tertiary hospital in Nepal.
A total of 2209 non-repetitive mid-stream urine (MSU) samples were collected during the study period (March to September 2014). Identification of the isolates was done by Gram's staining followed by biochemical tests. Antibiotic susceptibility testing was done by modified Kirby-Bauer disc diffusion method and interpretation was done following Clinical and Laboratory Standard Institute (CLSI) guidelines, 2013. ESBL screening among
and
spp. isolates were done using ceftriaxone, cefotaxime, ceftazidime and cefpodoxime. The confirmation was done by phenotypic disc diffusion test (combined disc method) using ceftazidime (30µg) and ceftazidime plus clavulanic acid (30/10µg), and cefotaxime (30µg) and cefotaxime plus clavulanic acid (30/10µg) disc as per CLSI guidelines.
A total of 451 samples showed significant bacteriuria with 365 (80.9%)
, 17 (3.8%)
and 3 (0.7%)
. Of 451 isolates, 236 (52.3%) were found MDR strains. By combined disk test, 33 (91.7%)
and 3 (8.3%)
spp. were found ESBL producers.
Higher prevalence of ESBL producing
and
spp. was observed warranting prompt need of surveillance for effective management of such MDR strains.</description><issn>1874-2858</issn><issn>1874-2858</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNptUU1LAzEQDaL4_QsEydFLa742m3gQ2lK1WFBoPYc0yWpku1mTXaH_3l2touBphjdv3rzhAXCG0ZDgnF1ikTMiMoFwjjHCCBG6Aw57dNDDu7_6A3CU0itCnEiJ9sFBh2WUYXYI_HQxnsPHGGxrGh8qOFqH6hlOh9CE0kNdWXhfulXyriw1THU9hBPdJt9xnqKvdNzAZdSmgbOqcJ8KV3AE70KqfaNLONbJWbhoWrs5AXuFLpM73dZj8HQzXU7uBvOH29lkNB8YKjgdMMGLzOUGZ1JgwikuCBcIUcoKJwjunK-45tYiy2TGRMGFMVhyJzlCbGUlPQbXX7p1u1o7a1zVRF2qOvp151YF7dXfSeVf1HN4VxkjORd5J3CxFYjhrXWpUWufTP9_5UKbFJaIMiokwR2VflFNDClFV_ycwUj1Ial_Quq2zn87_Nn5ToV-AJEziyA</recordid><startdate>20170428</startdate><enddate>20170428</enddate><creator>Shakya, Pooja</creator><creator>Shrestha, Dhiraj</creator><creator>Maharjan, Elina</creator><creator>Sharma, Vijay K</creator><creator>Paudyal, Rabin</creator><general>Bentham Open</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170428</creationdate><title>ESBL Production Among E. coli and Klebsiella spp. Causing Urinary Tract Infection: A Hospital Based Study</title><author>Shakya, Pooja ; Shrestha, Dhiraj ; Maharjan, Elina ; Sharma, Vijay K ; Paudyal, Rabin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3863-486f5e7c159812631f26800334fe821285b6a6dd0d49548f68cc196e96004bd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shakya, Pooja</creatorcontrib><creatorcontrib>Shrestha, Dhiraj</creatorcontrib><creatorcontrib>Maharjan, Elina</creatorcontrib><creatorcontrib>Sharma, Vijay K</creatorcontrib><creatorcontrib>Paudyal, Rabin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The open microbiology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shakya, Pooja</au><au>Shrestha, Dhiraj</au><au>Maharjan, Elina</au><au>Sharma, Vijay K</au><au>Paudyal, Rabin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ESBL Production Among E. coli and Klebsiella spp. Causing Urinary Tract Infection: A Hospital Based Study</atitle><jtitle>The open microbiology journal</jtitle><addtitle>Open Microbiol J</addtitle><date>2017-04-28</date><risdate>2017</risdate><volume>11</volume><issue>1</issue><spage>23</spage><epage>30</epage><pages>23-30</pages><issn>1874-2858</issn><eissn>1874-2858</eissn><abstract>Increase in extended-spectrum β-lactamases (ESBL) producing microbes in recent years has led to limitations of treatment options. This study aimed to assess the prevalence of ESBL producing
and
spp. at a tertiary hospital in Nepal.
A total of 2209 non-repetitive mid-stream urine (MSU) samples were collected during the study period (March to September 2014). Identification of the isolates was done by Gram's staining followed by biochemical tests. Antibiotic susceptibility testing was done by modified Kirby-Bauer disc diffusion method and interpretation was done following Clinical and Laboratory Standard Institute (CLSI) guidelines, 2013. ESBL screening among
and
spp. isolates were done using ceftriaxone, cefotaxime, ceftazidime and cefpodoxime. The confirmation was done by phenotypic disc diffusion test (combined disc method) using ceftazidime (30µg) and ceftazidime plus clavulanic acid (30/10µg), and cefotaxime (30µg) and cefotaxime plus clavulanic acid (30/10µg) disc as per CLSI guidelines.
A total of 451 samples showed significant bacteriuria with 365 (80.9%)
, 17 (3.8%)
and 3 (0.7%)
. Of 451 isolates, 236 (52.3%) were found MDR strains. By combined disk test, 33 (91.7%)
and 3 (8.3%)
spp. were found ESBL producers.
Higher prevalence of ESBL producing
and
spp. was observed warranting prompt need of surveillance for effective management of such MDR strains.</abstract><cop>United Arab Emirates</cop><pub>Bentham Open</pub><pmid>28553414</pmid><doi>10.2174/1874285801711010023</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| title | ESBL Production Among E. coli and Klebsiella spp. Causing Urinary Tract Infection: A Hospital Based Study |
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