A simple, less invasive stripper micropipetter-based technique for day 3 embryo biopsy
Preimplantation genetic screening (PGS) is an important procedure for in vitro fertilization (IVF). A key step of PGS, blastomere removal, is abundant with many technical issues. The aim of this study was to compare a more simple procedure based on the Stipper Micropipetter, named S-biopsy, to the c...
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| Veröffentlicht in: | Fertility Research and Practice 2016-11, Vol.2 (1), p.13-13, Article 13 |
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| creator | Cedillo, Luciano Ocampo-Bárcenas, Azucena Maldonado, Israel Valdez-Morales, Francisco J Camargo, Felipe López-Bayghen, Esther |
| description | Preimplantation genetic screening (PGS) is an important procedure for in vitro fertilization (IVF). A key step of PGS, blastomere removal, is abundant with many technical issues. The aim of this study was to compare a more simple procedure based on the Stipper Micropipetter, named S-biopsy, to the conventional aspiration method.
On Day 3, 368 high-quality embryos (>7 cells on Day3 with |
| doi_str_mv | 10.1186/s40738-016-0027-4 |
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On Day 3, 368 high-quality embryos (>7 cells on Day3 with <10% fragmentation) were collected from 38 women. For each patient, their embryos were equally separated between the conventional method (
= 188) and S-biopsy method (
= 180). The conventional method was performed using a standardized protocol. For the S-biopsy method, a laser was used to remove a significantly smaller portion of the zona pellucida. Afterwards, the complete embryo was aspirated with a Stripper Micropipetter, forcing the removal of the blastomere. Selected blastomeres went to PGS using CGH microarrays. Embryo integrity and blastocyst formation were assessed on Day 5. Differences between groups were assessed by either the Mann-Whitney test or Fisher Exact test.
Both methods resulted in the removal of only one blastomere. The S-biopsy and the conventional method did not differ in terms of affecting embryo integrity (95.0% vs. 95.7%) or blastocyst formation (72.7% vs. 70.7%). PGS analysis indicated that aneuploidy rate were similar between the two methods (63.1% vs. 65.2%). However, the time required to perform the S-biopsy method (179.2 ± 17.5 s) was significantly shorter (5-fold) than the conventional method.
The S-biopsy method is comparable to the conventional method that is used to remove a blastomere for PGS, but requires less time. Furthermore, due to the simplicity of the S-biopsy technique, this method is more ideal for IVF laboratories.</description><identifier>ISSN: 2054-7099</identifier><identifier>EISSN: 2054-7099</identifier><identifier>DOI: 10.1186/s40738-016-0027-4</identifier><identifier>PMID: 28620540</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Biopsy ; Comparative analysis ; Human embryo ; Methodology ; Methods ; Physiological aspects</subject><ispartof>Fertility Research and Practice, 2016-11, Vol.2 (1), p.13-13, Article 13</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><rights>The Author(s). 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3394-c249c850fa02752193b38a588ca75a897e8097892a4c3054c58afff2b36980303</citedby><cites>FETCH-LOGICAL-c3394-c249c850fa02752193b38a588ca75a897e8097892a4c3054c58afff2b36980303</cites><orcidid>0000-0002-2849-7587</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424395/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424395/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28620540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cedillo, Luciano</creatorcontrib><creatorcontrib>Ocampo-Bárcenas, Azucena</creatorcontrib><creatorcontrib>Maldonado, Israel</creatorcontrib><creatorcontrib>Valdez-Morales, Francisco J</creatorcontrib><creatorcontrib>Camargo, Felipe</creatorcontrib><creatorcontrib>López-Bayghen, Esther</creatorcontrib><title>A simple, less invasive stripper micropipetter-based technique for day 3 embryo biopsy</title><title>Fertility Research and Practice</title><addtitle>Fertil Res Pract</addtitle><description>Preimplantation genetic screening (PGS) is an important procedure for in vitro fertilization (IVF). A key step of PGS, blastomere removal, is abundant with many technical issues. The aim of this study was to compare a more simple procedure based on the Stipper Micropipetter, named S-biopsy, to the conventional aspiration method.
On Day 3, 368 high-quality embryos (>7 cells on Day3 with <10% fragmentation) were collected from 38 women. For each patient, their embryos were equally separated between the conventional method (
= 188) and S-biopsy method (
= 180). The conventional method was performed using a standardized protocol. For the S-biopsy method, a laser was used to remove a significantly smaller portion of the zona pellucida. Afterwards, the complete embryo was aspirated with a Stripper Micropipetter, forcing the removal of the blastomere. Selected blastomeres went to PGS using CGH microarrays. Embryo integrity and blastocyst formation were assessed on Day 5. Differences between groups were assessed by either the Mann-Whitney test or Fisher Exact test.
Both methods resulted in the removal of only one blastomere. The S-biopsy and the conventional method did not differ in terms of affecting embryo integrity (95.0% vs. 95.7%) or blastocyst formation (72.7% vs. 70.7%). PGS analysis indicated that aneuploidy rate were similar between the two methods (63.1% vs. 65.2%). However, the time required to perform the S-biopsy method (179.2 ± 17.5 s) was significantly shorter (5-fold) than the conventional method.
The S-biopsy method is comparable to the conventional method that is used to remove a blastomere for PGS, but requires less time. Furthermore, due to the simplicity of the S-biopsy technique, this method is more ideal for IVF laboratories.</description><subject>Biopsy</subject><subject>Comparative analysis</subject><subject>Human embryo</subject><subject>Methodology</subject><subject>Methods</subject><subject>Physiological aspects</subject><issn>2054-7099</issn><issn>2054-7099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdUU1r3DAQFaWlCUl-QC9F0EsPdTL6sqVLYQn9gkAvTa5C1o4SBdtyJe_C_vvKbBrSooOE5s2b9-YR8o7BJWO6vSoSOqEbYG0DwLtGviKnHJRsOjDm9Yv3Cbko5REAmNIdgH5LTrhu1zKckrsNLXGcB_xEByyFxmnvStwjLUuO84yZjtHnNMcZlwVz07uCW7qgf5ji7x3SkDLdugMVFMc-HxLtY5rL4Zy8CW4oePF0n5Hbr19-XX9vbn5--3G9uWm8EEY2nkvjtYLgqgPFmRG90E5p7V2nnDYdajCdNtxJL6pgr7QLIfBetEaDAHFGPh95510_4tbjtGQ32DnH0eWDTS7afytTfLD3aW-V5FIYVQk-PhHkVP2UxY6xeBwGN2HaFcsMAw0g2nXWh_-gj2mXp2rPMi2V4i0DVlGXR9S9G9DGKaQ619ezxbrJNGGI9X8jO6YMtGJVwI4Ndc2lZAzP6hnYNWl7TNrWpO2atJW15_1L288df3MVfwDkP6KY</recordid><startdate>20161125</startdate><enddate>20161125</enddate><creator>Cedillo, Luciano</creator><creator>Ocampo-Bárcenas, Azucena</creator><creator>Maldonado, Israel</creator><creator>Valdez-Morales, Francisco J</creator><creator>Camargo, Felipe</creator><creator>López-Bayghen, Esther</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2849-7587</orcidid></search><sort><creationdate>20161125</creationdate><title>A simple, less invasive stripper micropipetter-based technique for day 3 embryo biopsy</title><author>Cedillo, Luciano ; Ocampo-Bárcenas, Azucena ; Maldonado, Israel ; Valdez-Morales, Francisco J ; Camargo, Felipe ; López-Bayghen, Esther</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3394-c249c850fa02752193b38a588ca75a897e8097892a4c3054c58afff2b36980303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biopsy</topic><topic>Comparative analysis</topic><topic>Human embryo</topic><topic>Methodology</topic><topic>Methods</topic><topic>Physiological aspects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cedillo, Luciano</creatorcontrib><creatorcontrib>Ocampo-Bárcenas, Azucena</creatorcontrib><creatorcontrib>Maldonado, Israel</creatorcontrib><creatorcontrib>Valdez-Morales, Francisco J</creatorcontrib><creatorcontrib>Camargo, Felipe</creatorcontrib><creatorcontrib>López-Bayghen, Esther</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Fertility Research and Practice</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cedillo, Luciano</au><au>Ocampo-Bárcenas, Azucena</au><au>Maldonado, Israel</au><au>Valdez-Morales, Francisco J</au><au>Camargo, Felipe</au><au>López-Bayghen, Esther</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple, less invasive stripper micropipetter-based technique for day 3 embryo biopsy</atitle><jtitle>Fertility Research and Practice</jtitle><addtitle>Fertil Res Pract</addtitle><date>2016-11-25</date><risdate>2016</risdate><volume>2</volume><issue>1</issue><spage>13</spage><epage>13</epage><pages>13-13</pages><artnum>13</artnum><issn>2054-7099</issn><eissn>2054-7099</eissn><abstract>Preimplantation genetic screening (PGS) is an important procedure for in vitro fertilization (IVF). A key step of PGS, blastomere removal, is abundant with many technical issues. The aim of this study was to compare a more simple procedure based on the Stipper Micropipetter, named S-biopsy, to the conventional aspiration method.
On Day 3, 368 high-quality embryos (>7 cells on Day3 with <10% fragmentation) were collected from 38 women. For each patient, their embryos were equally separated between the conventional method (
= 188) and S-biopsy method (
= 180). The conventional method was performed using a standardized protocol. For the S-biopsy method, a laser was used to remove a significantly smaller portion of the zona pellucida. Afterwards, the complete embryo was aspirated with a Stripper Micropipetter, forcing the removal of the blastomere. Selected blastomeres went to PGS using CGH microarrays. Embryo integrity and blastocyst formation were assessed on Day 5. Differences between groups were assessed by either the Mann-Whitney test or Fisher Exact test.
Both methods resulted in the removal of only one blastomere. The S-biopsy and the conventional method did not differ in terms of affecting embryo integrity (95.0% vs. 95.7%) or blastocyst formation (72.7% vs. 70.7%). PGS analysis indicated that aneuploidy rate were similar between the two methods (63.1% vs. 65.2%). However, the time required to perform the S-biopsy method (179.2 ± 17.5 s) was significantly shorter (5-fold) than the conventional method.
The S-biopsy method is comparable to the conventional method that is used to remove a blastomere for PGS, but requires less time. Furthermore, due to the simplicity of the S-biopsy technique, this method is more ideal for IVF laboratories.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>28620540</pmid><doi>10.1186/s40738-016-0027-4</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2849-7587</orcidid><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Open Access; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Biopsy Comparative analysis Human embryo Methodology Methods Physiological aspects |
| title | A simple, less invasive stripper micropipetter-based technique for day 3 embryo biopsy |
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