Vitamin D increases programmed death receptor-1 expression in Crohn's disease
Vitamin D modulates inflammation in Crohn's disease (CD). Programmed death (PD)-1 receptor contributes to the maintenance of immune tolerance. Vitamin D might modulate PD-1 signalling in CD. To investigate PD-1 expression on T cell subsets in CD patients treated with vitamin D or placebo. We in...
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| Veröffentlicht in: | Oncotarget 2017-04, Vol.8 (15), p.24177-24186 |
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| creator | Bendix, Mia Greisen, Stinne Dige, Anders Hvas, Christian L Bak, Nina Jørgensen, Søren P Dahlerup, Jens F Deleuran, Bent Agnholt, Jørgen |
| description | Vitamin D modulates inflammation in Crohn's disease (CD). Programmed death (PD)-1 receptor contributes to the maintenance of immune tolerance. Vitamin D might modulate PD-1 signalling in CD.
To investigate PD-1 expression on T cell subsets in CD patients treated with vitamin D or placebo.
We included 40 CD patients who received 1200 IU vitamin D3 for 26 weeks or placebo and eight healthy controls. Peripheral blood mononuclear cells (PBMCs) and plasma were isolated at baseline and week 26. The expressions of PD-1, PD-L1, and surface activation markers were analysed by flow cytometry. Soluble PD-1 plasma levels were measured by ELISA.
PD-1 expression upon T cell stimulation was increased in CD4+CD25+int T cells in vitamin D treated CD patients from 19% (range 10 - 39%) to 29% (11 - 79%)(p = 0.03) compared with placebo-treated patients. Vitamin D treatment, but not placebo, decreased the expression of the T cell activation marker CD69 from 42% (31 - 62%) to 33% (19 - 54%)(p = 0.01). Soluble PD-1 levels were not influenced by vitamin D treatment.
Vitamin D treatment increases CD4+CD25+int T cells ability to up-regulate PD-1 in response to activation and reduces the CD69 expression in CD patients. |
| doi_str_mv | 10.18632/oncotarget.15489 |
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To investigate PD-1 expression on T cell subsets in CD patients treated with vitamin D or placebo.
We included 40 CD patients who received 1200 IU vitamin D3 for 26 weeks or placebo and eight healthy controls. Peripheral blood mononuclear cells (PBMCs) and plasma were isolated at baseline and week 26. The expressions of PD-1, PD-L1, and surface activation markers were analysed by flow cytometry. Soluble PD-1 plasma levels were measured by ELISA.
PD-1 expression upon T cell stimulation was increased in CD4+CD25+int T cells in vitamin D treated CD patients from 19% (range 10 - 39%) to 29% (11 - 79%)(p = 0.03) compared with placebo-treated patients. Vitamin D treatment, but not placebo, decreased the expression of the T cell activation marker CD69 from 42% (31 - 62%) to 33% (19 - 54%)(p = 0.01). Soluble PD-1 levels were not influenced by vitamin D treatment.
Vitamin D treatment increases CD4+CD25+int T cells ability to up-regulate PD-1 in response to activation and reduces the CD69 expression in CD patients.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.15489</identifier><identifier>PMID: 28412753</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Adult ; Aged ; Cells, Cultured ; Crohn Disease - drug therapy ; Crohn Disease - genetics ; Crohn Disease - immunology ; Crohn Disease - metabolism ; Female ; Gene Expression Regulation - drug effects ; Humans ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - metabolism ; Lymphocyte Activation - drug effects ; Lymphocyte Activation - genetics ; Lymphocyte Activation - immunology ; Male ; Middle Aged ; Programmed Cell Death 1 Receptor - genetics ; Research Paper: Immunology ; T-Lymphocyte Subsets - immunology ; T-Lymphocyte Subsets - metabolism ; Vitamin D - metabolism ; Vitamin D - pharmacology ; Vitamin D - therapeutic use ; Young Adult</subject><ispartof>Oncotarget, 2017-04, Vol.8 (15), p.24177-24186</ispartof><rights>Copyright: © 2017 Bendix et al. 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-72d59316322b989e11616e81b118f98a964530f92c243a38ed305d57ebe1d89c3</citedby><cites>FETCH-LOGICAL-c356t-72d59316322b989e11616e81b118f98a964530f92c243a38ed305d57ebe1d89c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421837/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5421837/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28412753$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bendix, Mia</creatorcontrib><creatorcontrib>Greisen, Stinne</creatorcontrib><creatorcontrib>Dige, Anders</creatorcontrib><creatorcontrib>Hvas, Christian L</creatorcontrib><creatorcontrib>Bak, Nina</creatorcontrib><creatorcontrib>Jørgensen, Søren P</creatorcontrib><creatorcontrib>Dahlerup, Jens F</creatorcontrib><creatorcontrib>Deleuran, Bent</creatorcontrib><creatorcontrib>Agnholt, Jørgen</creatorcontrib><title>Vitamin D increases programmed death receptor-1 expression in Crohn's disease</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>Vitamin D modulates inflammation in Crohn's disease (CD). Programmed death (PD)-1 receptor contributes to the maintenance of immune tolerance. Vitamin D might modulate PD-1 signalling in CD.
To investigate PD-1 expression on T cell subsets in CD patients treated with vitamin D or placebo.
We included 40 CD patients who received 1200 IU vitamin D3 for 26 weeks or placebo and eight healthy controls. Peripheral blood mononuclear cells (PBMCs) and plasma were isolated at baseline and week 26. The expressions of PD-1, PD-L1, and surface activation markers were analysed by flow cytometry. Soluble PD-1 plasma levels were measured by ELISA.
PD-1 expression upon T cell stimulation was increased in CD4+CD25+int T cells in vitamin D treated CD patients from 19% (range 10 - 39%) to 29% (11 - 79%)(p = 0.03) compared with placebo-treated patients. Vitamin D treatment, but not placebo, decreased the expression of the T cell activation marker CD69 from 42% (31 - 62%) to 33% (19 - 54%)(p = 0.01). Soluble PD-1 levels were not influenced by vitamin D treatment.
Vitamin D treatment increases CD4+CD25+int T cells ability to up-regulate PD-1 in response to activation and reduces the CD69 expression in CD patients.</description><subject>Adult</subject><subject>Aged</subject><subject>Cells, Cultured</subject><subject>Crohn Disease - drug therapy</subject><subject>Crohn Disease - genetics</subject><subject>Crohn Disease - immunology</subject><subject>Crohn Disease - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - drug effects</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Lymphocyte Activation - drug effects</subject><subject>Lymphocyte Activation - genetics</subject><subject>Lymphocyte Activation - immunology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Programmed Cell Death 1 Receptor - genetics</subject><subject>Research Paper: Immunology</subject><subject>T-Lymphocyte Subsets - immunology</subject><subject>T-Lymphocyte Subsets - metabolism</subject><subject>Vitamin D - metabolism</subject><subject>Vitamin D - pharmacology</subject><subject>Vitamin D - therapeutic use</subject><subject>Young Adult</subject><issn>1949-2553</issn><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1PwzAMjRCITWM_gAvqDS4dddK0yQUJjU9piAtwjdLU3YLWpiQdgn9P2QYMX2zJfs_PfoQcQzIBkTF67hrjOu3n2E2Ap0LukSHIVMaUc7a_Uw_IOITXpA-e5oLKQzKgIgWaczYkDy-207VtoqvINsajDhii1ru513WNZVSi7haRR4Nt53wMEX60HkOwrukB0dS7RXMaotKGb-gROaj0MuB4m0fk-eb6aXoXzx5v76eXs9gwnnVxTksuGfRH0EIKiQAZZCigABCVFFpmKWdJJamhKdNMYMkSXvIcC4RSSMNG5GLD266KXqXBpvN6qVpva-0_ldNW_e80dqHm7l3xlIJgeU9wtiXw7m2FoVO1DQaXS92gWwUFQgjJeS5FPwqbUeNdCB6r3zWQqLUT6s8JtXaix5zs6vtF_PydfQHNMYeM</recordid><startdate>20170411</startdate><enddate>20170411</enddate><creator>Bendix, Mia</creator><creator>Greisen, Stinne</creator><creator>Dige, Anders</creator><creator>Hvas, Christian L</creator><creator>Bak, Nina</creator><creator>Jørgensen, Søren P</creator><creator>Dahlerup, Jens F</creator><creator>Deleuran, Bent</creator><creator>Agnholt, Jørgen</creator><general>Impact Journals LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170411</creationdate><title>Vitamin D increases programmed death receptor-1 expression in Crohn's disease</title><author>Bendix, Mia ; Greisen, Stinne ; Dige, Anders ; Hvas, Christian L ; Bak, Nina ; Jørgensen, Søren P ; Dahlerup, Jens F ; Deleuran, Bent ; Agnholt, Jørgen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-72d59316322b989e11616e81b118f98a964530f92c243a38ed305d57ebe1d89c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Cells, Cultured</topic><topic>Crohn Disease - drug therapy</topic><topic>Crohn Disease - genetics</topic><topic>Crohn Disease - immunology</topic><topic>Crohn Disease - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - drug effects</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Lymphocyte Activation - drug effects</topic><topic>Lymphocyte Activation - genetics</topic><topic>Lymphocyte Activation - immunology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Programmed Cell Death 1 Receptor - genetics</topic><topic>Research Paper: Immunology</topic><topic>T-Lymphocyte Subsets - immunology</topic><topic>T-Lymphocyte Subsets - metabolism</topic><topic>Vitamin D - metabolism</topic><topic>Vitamin D - pharmacology</topic><topic>Vitamin D - therapeutic use</topic><topic>Young Adult</topic><toplevel>online_resources</toplevel><creatorcontrib>Bendix, Mia</creatorcontrib><creatorcontrib>Greisen, Stinne</creatorcontrib><creatorcontrib>Dige, Anders</creatorcontrib><creatorcontrib>Hvas, Christian L</creatorcontrib><creatorcontrib>Bak, Nina</creatorcontrib><creatorcontrib>Jørgensen, Søren P</creatorcontrib><creatorcontrib>Dahlerup, Jens F</creatorcontrib><creatorcontrib>Deleuran, Bent</creatorcontrib><creatorcontrib>Agnholt, Jørgen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bendix, Mia</au><au>Greisen, Stinne</au><au>Dige, Anders</au><au>Hvas, Christian L</au><au>Bak, Nina</au><au>Jørgensen, Søren P</au><au>Dahlerup, Jens F</au><au>Deleuran, Bent</au><au>Agnholt, Jørgen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vitamin D increases programmed death receptor-1 expression in Crohn's disease</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2017-04-11</date><risdate>2017</risdate><volume>8</volume><issue>15</issue><spage>24177</spage><epage>24186</epage><pages>24177-24186</pages><issn>1949-2553</issn><eissn>1949-2553</eissn><abstract>Vitamin D modulates inflammation in Crohn's disease (CD). Programmed death (PD)-1 receptor contributes to the maintenance of immune tolerance. Vitamin D might modulate PD-1 signalling in CD.
To investigate PD-1 expression on T cell subsets in CD patients treated with vitamin D or placebo.
We included 40 CD patients who received 1200 IU vitamin D3 for 26 weeks or placebo and eight healthy controls. Peripheral blood mononuclear cells (PBMCs) and plasma were isolated at baseline and week 26. The expressions of PD-1, PD-L1, and surface activation markers were analysed by flow cytometry. Soluble PD-1 plasma levels were measured by ELISA.
PD-1 expression upon T cell stimulation was increased in CD4+CD25+int T cells in vitamin D treated CD patients from 19% (range 10 - 39%) to 29% (11 - 79%)(p = 0.03) compared with placebo-treated patients. Vitamin D treatment, but not placebo, decreased the expression of the T cell activation marker CD69 from 42% (31 - 62%) to 33% (19 - 54%)(p = 0.01). Soluble PD-1 levels were not influenced by vitamin D treatment.
Vitamin D treatment increases CD4+CD25+int T cells ability to up-regulate PD-1 in response to activation and reduces the CD69 expression in CD patients.</abstract><cop>United States</cop><pub>Impact Journals LLC</pub><pmid>28412753</pmid><doi>10.18632/oncotarget.15489</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Cells, Cultured Crohn Disease - drug therapy Crohn Disease - genetics Crohn Disease - immunology Crohn Disease - metabolism Female Gene Expression Regulation - drug effects Humans Leukocytes, Mononuclear - drug effects Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism Lymphocyte Activation - drug effects Lymphocyte Activation - genetics Lymphocyte Activation - immunology Male Middle Aged Programmed Cell Death 1 Receptor - genetics Research Paper: Immunology T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism Vitamin D - metabolism Vitamin D - pharmacology Vitamin D - therapeutic use Young Adult |
| title | Vitamin D increases programmed death receptor-1 expression in Crohn's disease |
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