Differential Plasmid Rescue From Transgenic Mouse DNAs Into Escherichia coli Methylation-Restriction Mutants

Plasmids comprising transgene insertions in four lines of transgenic mice have been retrieved by plasmid rescue into a set of Escherichia coli strains with mutations in different members of the methylation-dependent restriction system (MDRS). Statistical analysis of plasmid rescue frequencies has re...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1990-06, Vol.87 (12), p.4645-4649
Hauptverfasser:	Seth G. N. Grant, Jessee, Joel, Bloom, Fredric R., Hanahan, Douglas
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Chromosome Mapping DNA DNA - genetics DNA Transposable Elements Enzymes Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes Genetic engineering Genetic loci Genetic mutation Genetic technics Genotype Methods. Procedures. Technologies Methylation Mice Mice, Transgenic Mutation Plasmids Plasmids - genetics Restriction Mapping Transgenes Transgenic animals Transgenic animals and transgenic plants
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