Co-Administration of Vitamin E and Testosterone Attenuates The Atrazine-Induced Toxic Effects on Sperm Quality and Testes in Rats

Co-Administration of Vitamin E and Testosterone Attenuates The Atrazine-Induced Toxic Effects on Sperm Quality and Testes in Rats

OBJECTIVEAtrazine (ATZ) as a widely used herbicide is considered as a potent endocrine disrupter which adversely affects reproductive systems in both genders. This study aimed to assess the effects of testosterone (T)- and vitamin E (VitE)- alone and their coadministration on testicular function and...

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Veröffentlicht in:Cell journal (Yakhteh) 2017-06, Vol.19 (2), p.292-305
Hauptverfasser: Rezaie Agdam, Hamed, Razi, Mazdak, Amniattalab, Amir, Malekinejad, Hassan, Molavi, Morteza
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container_end_page 305
container_issue 2
container_start_page 292
container_title Cell journal (Yakhteh)
container_volume 19
creator Rezaie Agdam, Hamed
Razi, Mazdak
Amniattalab, Amir
Malekinejad, Hassan
Molavi, Morteza
description OBJECTIVEAtrazine (ATZ) as a widely used herbicide is considered as a potent endocrine disrupter which adversely affects reproductive systems in both genders. This study aimed to assess the effects of testosterone (T)- and vitamin E (VitE)- alone and their coadministration on testicular function and sperm parameters after exposure to ATZ in rats. MATERIALS AND METHODSIn this experimental study, the rats (n=30) are assigned into the following 5 groups: control-sham group (n=6) receiving corn oil, ATZ group (n=6) receiving 200 mg/kg ATZ alone, ATZ+VitE group (n=6) receiving 150 mg/kg ATZ+VitE, ATZ+T group (n=6) receiving 400 µg/kg ATZ+T, and ATZ+VitE+T group (n=6) receiving ATZ+VitE+T for 48 consecutive days. Total antioxidant capacity (TAC), total thiol molecules (TTM), and malondialdehyde (MDA) were analyzed. Serum levels of T, luteinizing hormone (LH), and inhibin-B (IN-B) were also determined. Histological examination and sperm analysis were performed. The data were analyzed using Graph-Pad Prism software version 2.01. RESULTSCo-administration of VitE and T significantly (P
doi_str_mv 10.22074/cellj.2016.490
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This study aimed to assess the effects of testosterone (T)- and vitamin E (VitE)- alone and their coadministration on testicular function and sperm parameters after exposure to ATZ in rats. MATERIALS AND METHODSIn this experimental study, the rats (n=30) are assigned into the following 5 groups: control-sham group (n=6) receiving corn oil, ATZ group (n=6) receiving 200 mg/kg ATZ alone, ATZ+VitE group (n=6) receiving 150 mg/kg ATZ+VitE, ATZ+T group (n=6) receiving 400 µg/kg ATZ+T, and ATZ+VitE+T group (n=6) receiving ATZ+VitE+T for 48 consecutive days. Total antioxidant capacity (TAC), total thiol molecules (TTM), and malondialdehyde (MDA) were analyzed. Serum levels of T, luteinizing hormone (LH), and inhibin-B (IN-B) were also determined. Histological examination and sperm analysis were performed. The data were analyzed using Graph-Pad Prism software version 2.01. RESULTSCo-administration of VitE and T significantly (P&lt;0.05) increased ATZ-decreased TAC and TTM levels and reduced ATZ-increased MDA content. T and VitE significantly (P&lt;0.05) increased serum levels of ATZ-reduced T (1.94 ± 0.96), IN-B (122.10 ± 24.33) and LH (0.40 ± 0.10). The T+VitE animals showed a reduction in apoptotic cells and an increase in Leydig cells steroidogenesis. Co-administration of T and VitE significantly (P&lt;0.05) reduced the ATZ-induced DNA disintegrity and chromatin de-condensation. VitE and T protected germinal cells RNA and protein contents against ATZ-induced damages. CONCLUSIONT and VitE in simultaneous form of administration were able to normalize the ATZ-induced derangements through promoting antioxidant capacity and endocrine function.</description><identifier>ISSN: 2228-5806</identifier><identifier>EISSN: 2228-5814</identifier><identifier>DOI: 10.22074/cellj.2016.490</identifier><identifier>PMID: 28670522</identifier><language>eng</language><publisher>Royan Institute</publisher><subject>Original</subject><ispartof>Cell journal (Yakhteh), 2017-06, Vol.19 (2), p.292-305</ispartof><rights>Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412788/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5412788/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Rezaie Agdam, Hamed</creatorcontrib><creatorcontrib>Razi, Mazdak</creatorcontrib><creatorcontrib>Amniattalab, Amir</creatorcontrib><creatorcontrib>Malekinejad, Hassan</creatorcontrib><creatorcontrib>Molavi, Morteza</creatorcontrib><title>Co-Administration of Vitamin E and Testosterone Attenuates The Atrazine-Induced Toxic Effects on Sperm Quality and Testes in Rats</title><title>Cell journal (Yakhteh)</title><description>OBJECTIVEAtrazine (ATZ) as a widely used herbicide is considered as a potent endocrine disrupter which adversely affects reproductive systems in both genders. This study aimed to assess the effects of testosterone (T)- and vitamin E (VitE)- alone and their coadministration on testicular function and sperm parameters after exposure to ATZ in rats. MATERIALS AND METHODSIn this experimental study, the rats (n=30) are assigned into the following 5 groups: control-sham group (n=6) receiving corn oil, ATZ group (n=6) receiving 200 mg/kg ATZ alone, ATZ+VitE group (n=6) receiving 150 mg/kg ATZ+VitE, ATZ+T group (n=6) receiving 400 µg/kg ATZ+T, and ATZ+VitE+T group (n=6) receiving ATZ+VitE+T for 48 consecutive days. Total antioxidant capacity (TAC), total thiol molecules (TTM), and malondialdehyde (MDA) were analyzed. Serum levels of T, luteinizing hormone (LH), and inhibin-B (IN-B) were also determined. Histological examination and sperm analysis were performed. The data were analyzed using Graph-Pad Prism software version 2.01. RESULTSCo-administration of VitE and T significantly (P&lt;0.05) increased ATZ-decreased TAC and TTM levels and reduced ATZ-increased MDA content. T and VitE significantly (P&lt;0.05) increased serum levels of ATZ-reduced T (1.94 ± 0.96), IN-B (122.10 ± 24.33) and LH (0.40 ± 0.10). The T+VitE animals showed a reduction in apoptotic cells and an increase in Leydig cells steroidogenesis. Co-administration of T and VitE significantly (P&lt;0.05) reduced the ATZ-induced DNA disintegrity and chromatin de-condensation. VitE and T protected germinal cells RNA and protein contents against ATZ-induced damages. CONCLUSIONT and VitE in simultaneous form of administration were able to normalize the ATZ-induced derangements through promoting antioxidant capacity and endocrine function.</description><subject>Original</subject><issn>2228-5806</issn><issn>2228-5814</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpVkc9r2zAUx8XoWEKX86469uJMepZs5VIIId0ChbIt29XI8lOrYEupJZdlt_7nVVkJ9F3e7w_vyyPkC2dLAFaLrwb7_rAExqulWLEPZA4AqpCKi4tzzKoZWcR4YNkqBnwFn8gMVFUzCTAnz5tQrLvBeRfTqJMLngZL_7ikc41uqfYd3WNMISYcg0e6Tgn9pBNGun94TUf9z3ksdr6bDObh8NcZurUWTYo0434dcRzoj0n3Lp3OvLye-T91ip_JR6v7iIs3f0l-32z3m-_F7d233WZ9WxxBlKkwJRfKakDTttjaVtXlSvEKmMkSdc2tsIAaKi5sDYAoOyHbUvGuk6oznJWX5Po_9zi1A3YGfT69b46jG_R4aoJ2zfuOdw_NfXhqpOBQK5UBV2-AMTxOWUMzuPj6Au0xTLHhKy6lrGUpyxfY5H8Y</recordid><startdate>20170601</startdate><enddate>20170601</enddate><creator>Rezaie Agdam, Hamed</creator><creator>Razi, Mazdak</creator><creator>Amniattalab, Amir</creator><creator>Malekinejad, Hassan</creator><creator>Molavi, Morteza</creator><general>Royan Institute</general><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170601</creationdate><title>Co-Administration of Vitamin E and Testosterone Attenuates The Atrazine-Induced Toxic Effects on Sperm Quality and Testes in Rats</title><author>Rezaie Agdam, Hamed ; Razi, Mazdak ; Amniattalab, Amir ; Malekinejad, Hassan ; Molavi, Morteza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p243t-c3148fa2ecbbebfb873981620c806a71f4f2ea2614f722ee5d45b381dd58dc103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Original</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rezaie Agdam, Hamed</creatorcontrib><creatorcontrib>Razi, Mazdak</creatorcontrib><creatorcontrib>Amniattalab, Amir</creatorcontrib><creatorcontrib>Malekinejad, Hassan</creatorcontrib><creatorcontrib>Molavi, Morteza</creatorcontrib><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell journal (Yakhteh)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rezaie Agdam, Hamed</au><au>Razi, Mazdak</au><au>Amniattalab, Amir</au><au>Malekinejad, Hassan</au><au>Molavi, Morteza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Co-Administration of Vitamin E and Testosterone Attenuates The Atrazine-Induced Toxic Effects on Sperm Quality and Testes in Rats</atitle><jtitle>Cell journal (Yakhteh)</jtitle><date>2017-06-01</date><risdate>2017</risdate><volume>19</volume><issue>2</issue><spage>292</spage><epage>305</epage><pages>292-305</pages><issn>2228-5806</issn><eissn>2228-5814</eissn><abstract>OBJECTIVEAtrazine (ATZ) as a widely used herbicide is considered as a potent endocrine disrupter which adversely affects reproductive systems in both genders. This study aimed to assess the effects of testosterone (T)- and vitamin E (VitE)- alone and their coadministration on testicular function and sperm parameters after exposure to ATZ in rats. MATERIALS AND METHODSIn this experimental study, the rats (n=30) are assigned into the following 5 groups: control-sham group (n=6) receiving corn oil, ATZ group (n=6) receiving 200 mg/kg ATZ alone, ATZ+VitE group (n=6) receiving 150 mg/kg ATZ+VitE, ATZ+T group (n=6) receiving 400 µg/kg ATZ+T, and ATZ+VitE+T group (n=6) receiving ATZ+VitE+T for 48 consecutive days. Total antioxidant capacity (TAC), total thiol molecules (TTM), and malondialdehyde (MDA) were analyzed. Serum levels of T, luteinizing hormone (LH), and inhibin-B (IN-B) were also determined. Histological examination and sperm analysis were performed. The data were analyzed using Graph-Pad Prism software version 2.01. RESULTSCo-administration of VitE and T significantly (P&lt;0.05) increased ATZ-decreased TAC and TTM levels and reduced ATZ-increased MDA content. T and VitE significantly (P&lt;0.05) increased serum levels of ATZ-reduced T (1.94 ± 0.96), IN-B (122.10 ± 24.33) and LH (0.40 ± 0.10). The T+VitE animals showed a reduction in apoptotic cells and an increase in Leydig cells steroidogenesis. Co-administration of T and VitE significantly (P&lt;0.05) reduced the ATZ-induced DNA disintegrity and chromatin de-condensation. VitE and T protected germinal cells RNA and protein contents against ATZ-induced damages. CONCLUSIONT and VitE in simultaneous form of administration were able to normalize the ATZ-induced derangements through promoting antioxidant capacity and endocrine function.</abstract><pub>Royan Institute</pub><pmid>28670522</pmid><doi>10.22074/cellj.2016.490</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record>
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title Co-Administration of Vitamin E and Testosterone Attenuates The Atrazine-Induced Toxic Effects on Sperm Quality and Testes in Rats
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