Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice
Ovarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are ve...
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| Veröffentlicht in: | Journal of Visualized Experiments 2017-02 (120) |
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| creator | Li, Shan-Shan Ip, Carman K. M. Tang, Matthew Y. H. Sy, Samuel K. H. Yung, Susan Chan, Tak-Mao Yang, Mengsu Shum, Ho Cheung Wong, Alice S.T. |
| description | Ovarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development. |
| doi_str_mv | 10.3791/55337 |
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M. ; Tang, Matthew Y. H. ; Sy, Samuel K. H. ; Yung, Susan ; Chan, Tak-Mao ; Yang, Mengsu ; Shum, Ho Cheung ; Wong, Alice S.T.</creator><creatorcontrib>Li, Shan-Shan ; Ip, Carman K. M. ; Tang, Matthew Y. H. ; Sy, Samuel K. H. ; Yung, Susan ; Chan, Tak-Mao ; Yang, Mengsu ; Shum, Ho Cheung ; Wong, Alice S.T.</creatorcontrib><description>Ovarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development.</description><identifier>ISSN: 1940-087X</identifier><identifier>EISSN: 1940-087X</identifier><identifier>DOI: 10.3791/55337</identifier><identifier>PMID: 28287578</identifier><language>eng</language><publisher>United States: MyJove Corporation</publisher><subject>Bioengineering ; Cell Line, Tumor ; Disease Progression ; Epithelium - pathology ; Female ; Humans ; Microfluidic Analytical Techniques - methods ; Models, Biological ; Neoplasm Metastasis - diagnosis ; Ovarian Neoplasms - pathology ; Peritoneal Cavity - pathology ; Peritoneal Neoplasms - diagnosis ; Peritoneal Neoplasms - secondary ; Spheroids, Cellular - pathology ; Tumor Microenvironment</subject><ispartof>Journal of Visualized Experiments, 2017-02 (120)</ispartof><rights>Copyright © 2017, Journal of Visualized Experiments</rights><rights>Copyright © 2017, Journal of Visualized Experiments 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-e36b0701bf70b1388f6e78871e290d40d7355a02ed32f08304ba77a8d71e59153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttps://www.jove.com/files/email_thumbs/55337.png</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409276/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5409276/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3830,27901,27902,53766,53768</link.rule.ids><linktorsrc>$$Uhttp://dx.doi.org/10.3791/55337$$EView_record_in_Journal_of_Visualized_Experiments$$FView_record_in_$$GJournal_of_Visualized_Experiments</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28287578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Shan-Shan</creatorcontrib><creatorcontrib>Ip, Carman K. M.</creatorcontrib><creatorcontrib>Tang, Matthew Y. H.</creatorcontrib><creatorcontrib>Sy, Samuel K. H.</creatorcontrib><creatorcontrib>Yung, Susan</creatorcontrib><creatorcontrib>Chan, Tak-Mao</creatorcontrib><creatorcontrib>Yang, Mengsu</creatorcontrib><creatorcontrib>Shum, Ho Cheung</creatorcontrib><creatorcontrib>Wong, Alice S.T.</creatorcontrib><title>Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice</title><title>Journal of Visualized Experiments</title><addtitle>J Vis Exp</addtitle><description>Ovarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development.</description><subject>Bioengineering</subject><subject>Cell Line, Tumor</subject><subject>Disease Progression</subject><subject>Epithelium - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Microfluidic Analytical Techniques - methods</subject><subject>Models, Biological</subject><subject>Neoplasm Metastasis - diagnosis</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Peritoneal Cavity - pathology</subject><subject>Peritoneal Neoplasms - diagnosis</subject><subject>Peritoneal Neoplasms - secondary</subject><subject>Spheroids, Cellular - pathology</subject><subject>Tumor Microenvironment</subject><issn>1940-087X</issn><issn>1940-087X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1LAzEQhoMofv8BD5KL4KU62Ww22Yug9RNaKqjgyZDdnbUp26RmP8B_79ZW0dMMzDPvzDtDyCGDMy5Tdi4E53KD7LI0hgEo-br5J98he3U9A0giEGqb7EQqUlJItUvexr7Ayrp3OulMsMbRoXE5Bjpuq8bmWFVtZQJ9WkwxeFvQK5yazvpAraOGXn86M7c55df0EYNtvENT0bHNQ6_a9e0HZKs0VY2H67hPXm5vnof3g9Hk7mF4ORrkcayaAfIkAwksKyVkjCtVJiiVkgyjFIoYCsmFMBBhwaMSFIc4M1IaVfSESJng--RipbtoszkWObommEovgp2b8Km9sfp_xdmpfvedFjGkkUx6gdO1QPAfLdaNntt6ad849G2tmZKJYjGI5ayTFdq7rOuA5e8YBnr5C_39i547_rvTL_Vz_B44WgEz36Ge-Ta4_kbr7i_6UIye</recordid><startdate>20170218</startdate><enddate>20170218</enddate><creator>Li, Shan-Shan</creator><creator>Ip, Carman K. 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H.</au><au>Sy, Samuel K. H.</au><au>Yung, Susan</au><au>Chan, Tak-Mao</au><au>Yang, Mengsu</au><au>Shum, Ho Cheung</au><au>Wong, Alice S.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice</atitle><jtitle>Journal of Visualized Experiments</jtitle><addtitle>J Vis Exp</addtitle><date>2017-02-18</date><risdate>2017</risdate><issue>120</issue><issn>1940-087X</issn><eissn>1940-087X</eissn><abstract>Ovarian cancer is characterized by extensive peritoneal metastasis, with tumor spheres commonly found in the malignant ascites. This is associated with poor clinical outcomes and currently lacks effective treatment. Both the three-dimensional (3D) environment and the dynamic mechanical forces are very important factors in this metastatic cascade. However, traditional cell cultures fail to recapitulate this natural tumor microenvironment. Thus, in vivo-like models that can emulate the intraperitoneal environment are of obvious importance. In this study, a new microfluidic platform of the peritoneum was set up to mimic the situation of ovarian cancer spheroids in the peritoneal cavity during metastasis. Ovarian cancer spheroids generated under a non-adherent condition were cultured in microfluidic channels coated with peritoneal mesothelial cells subjected to physiologically relevant shear stress. In summary, this dynamic 3D ovarian cancer-mesothelium microfluidic platform can provide new knowledge on basic cancer biology and serve as a platform for potential drug screening and development.</abstract><cop>United States</cop><pub>MyJove Corporation</pub><pmid>28287578</pmid><doi>10.3791/55337</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Bioengineering Cell Line, Tumor Disease Progression Epithelium - pathology Female Humans Microfluidic Analytical Techniques - methods Models, Biological Neoplasm Metastasis - diagnosis Ovarian Neoplasms - pathology Peritoneal Cavity - pathology Peritoneal Neoplasms - diagnosis Peritoneal Neoplasms - secondary Spheroids, Cellular - pathology Tumor Microenvironment |
| title | Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice |
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