Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model
Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a parti...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2017-04, Vol.7 (1), p.46686, Article 46686 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | 46686 |
| container_title | Scientific reports |
| container_volume | 7 |
| creator | Khodanovich, Marina Yu Sorokina, Irina V. Glazacheva, Valentina Yu Akulov, Andrey E. Nemirovich-Danchenko, Nikolay M. Romashchenko, Alexander V. Tolstikova, Tatyana G. Mustafina, Lilia R. Yarnykh, Vasily L. |
| description | Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of sensitivity to both white matter (WM) and gray matter (GM) demyelination. This study aimed to histologically validate the capability of MPF mapping to quantify myelin loss in brain tissues using the cuprizone demyelination model. Whole-brain MPF maps were obtained
in vivo
on an 11.7T animal MRI scanner from 7 cuprizone-treated and 7 control С57BL/6 mice using the fast single-point synthetic-reference method. Brain sections were histologically stained with Luxol Fast Blue (LFB) for myelin quantification. Significant (p |
| doi_str_mv | 10.1038/srep46686 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5402392</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1903496289</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-e72129f3453a74a7e17ec49b94e2fe378ac08ff785d8e2a224e6b929f72f41783</originalsourceid><addsrcrecordid>eNplkU9rFTEUxQdRbKld-AUk4Erh2UyS-ZONIEWtUOimXYf7MjfzUjLJNMk8qF_DL2zq1McTs0nI-eXcQ05Vva3pp5ry_iJFnEXb9u2L6pRR0WwYZ-zl0fmkOk_pnpbVMClq-bo6Yb3grWjpafXryqYcXBitBkf24OwA2QZPgiEGUiYT6Bim4FAvDiKZY8hFNRH0H2yCebZ-JJAIkIcFfLa5GOyRTI_orCd2gvEJmDDvwkDKTd4h0csc7c_gkQy4guvUKQzo3lSvDLiE58_7WXX37evt5dXm-ub7j8sv1xvdUJE32LGaScNFw6ET0GHdoRZyKwUyg7zrQdPemK5vhh4ZMCaw3cryomNG1F3Pz6rPq--8bCccNPocwamSbIL4qAJY9a_i7U6NYa8aQRmXrBi8fzaI4WHBlNV9WKIvmVUtKReyZb0s1IeVKh-ZSlvmMKGm6qlCdaiwsO-OIx3Iv4UV4OMKpCL5EePRyP_cfgPUnKpb</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1903496289</pqid></control><display><type>article</type><title>Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model</title><source>DOAJ Directory of Open Access Journals</source><source>Nature Journals Online</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Khodanovich, Marina Yu ; Sorokina, Irina V. ; Glazacheva, Valentina Yu ; Akulov, Andrey E. ; Nemirovich-Danchenko, Nikolay M. ; Romashchenko, Alexander V. ; Tolstikova, Tatyana G. ; Mustafina, Lilia R. ; Yarnykh, Vasily L.</creator><creatorcontrib>Khodanovich, Marina Yu ; Sorokina, Irina V. ; Glazacheva, Valentina Yu ; Akulov, Andrey E. ; Nemirovich-Danchenko, Nikolay M. ; Romashchenko, Alexander V. ; Tolstikova, Tatyana G. ; Mustafina, Lilia R. ; Yarnykh, Vasily L.</creatorcontrib><description>Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of sensitivity to both white matter (WM) and gray matter (GM) demyelination. This study aimed to histologically validate the capability of MPF mapping to quantify myelin loss in brain tissues using the cuprizone demyelination model. Whole-brain MPF maps were obtained
in vivo
on an 11.7T animal MRI scanner from 7 cuprizone-treated and 7 control С57BL/6 mice using the fast single-point synthetic-reference method. Brain sections were histologically stained with Luxol Fast Blue (LFB) for myelin quantification. Significant (p < 0.05) demyelination in cuprizone-treated animals was found according to both LFB staining and MPF in all anatomical structures (corpus callosum, anterior commissure, internal capsule, thalamus, caudoputamen, and cortex). MPF strongly correlated with quantitative histology in all animals (r = 0.95, p < 0.001) as well as in treatment and control groups taken separately (r = 0.96, p = 0.002 and r = 0.93, p = 0.007, respectively). Close agreement between histological myelin staining and MPF suggests that fast MPF mapping enables robust and accurate quantitative assessment of demyelination in both WM and GM.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep46686</identifier><identifier>PMID: 28436460</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/51 ; 631/1647/245/1628 ; 631/378/2606/1666 ; Animals ; Anterior commissure ; Biomarkers ; Brain ; Brain mapping ; Corpus callosum ; Cuprizone ; Demyelination ; Histology ; Humanities and Social Sciences ; Macromolecules ; Magnetic resonance imaging ; multidisciplinary ; Multiple sclerosis ; Myelin ; Neuroimaging ; NMR ; Nuclear magnetic resonance ; Regions ; Regression analysis ; Science ; Substantia alba ; Substantia grisea ; Thalamus ; Variance analysis</subject><ispartof>Scientific reports, 2017-04, Vol.7 (1), p.46686, Article 46686</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Apr 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-e72129f3453a74a7e17ec49b94e2fe378ac08ff785d8e2a224e6b929f72f41783</citedby><cites>FETCH-LOGICAL-c504t-e72129f3453a74a7e17ec49b94e2fe378ac08ff785d8e2a224e6b929f72f41783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402392/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402392/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28436460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khodanovich, Marina Yu</creatorcontrib><creatorcontrib>Sorokina, Irina V.</creatorcontrib><creatorcontrib>Glazacheva, Valentina Yu</creatorcontrib><creatorcontrib>Akulov, Andrey E.</creatorcontrib><creatorcontrib>Nemirovich-Danchenko, Nikolay M.</creatorcontrib><creatorcontrib>Romashchenko, Alexander V.</creatorcontrib><creatorcontrib>Tolstikova, Tatyana G.</creatorcontrib><creatorcontrib>Mustafina, Lilia R.</creatorcontrib><creatorcontrib>Yarnykh, Vasily L.</creatorcontrib><title>Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of sensitivity to both white matter (WM) and gray matter (GM) demyelination. This study aimed to histologically validate the capability of MPF mapping to quantify myelin loss in brain tissues using the cuprizone demyelination model. Whole-brain MPF maps were obtained
in vivo
on an 11.7T animal MRI scanner from 7 cuprizone-treated and 7 control С57BL/6 mice using the fast single-point synthetic-reference method. Brain sections were histologically stained with Luxol Fast Blue (LFB) for myelin quantification. Significant (p < 0.05) demyelination in cuprizone-treated animals was found according to both LFB staining and MPF in all anatomical structures (corpus callosum, anterior commissure, internal capsule, thalamus, caudoputamen, and cortex). MPF strongly correlated with quantitative histology in all animals (r = 0.95, p < 0.001) as well as in treatment and control groups taken separately (r = 0.96, p = 0.002 and r = 0.93, p = 0.007, respectively). Close agreement between histological myelin staining and MPF suggests that fast MPF mapping enables robust and accurate quantitative assessment of demyelination in both WM and GM.</description><subject>13/51</subject><subject>631/1647/245/1628</subject><subject>631/378/2606/1666</subject><subject>Animals</subject><subject>Anterior commissure</subject><subject>Biomarkers</subject><subject>Brain</subject><subject>Brain mapping</subject><subject>Corpus callosum</subject><subject>Cuprizone</subject><subject>Demyelination</subject><subject>Histology</subject><subject>Humanities and Social Sciences</subject><subject>Macromolecules</subject><subject>Magnetic resonance imaging</subject><subject>multidisciplinary</subject><subject>Multiple sclerosis</subject><subject>Myelin</subject><subject>Neuroimaging</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Regions</subject><subject>Regression analysis</subject><subject>Science</subject><subject>Substantia alba</subject><subject>Substantia grisea</subject><subject>Thalamus</subject><subject>Variance analysis</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNplkU9rFTEUxQdRbKld-AUk4Erh2UyS-ZONIEWtUOimXYf7MjfzUjLJNMk8qF_DL2zq1McTs0nI-eXcQ05Vva3pp5ry_iJFnEXb9u2L6pRR0WwYZ-zl0fmkOk_pnpbVMClq-bo6Yb3grWjpafXryqYcXBitBkf24OwA2QZPgiEGUiYT6Bim4FAvDiKZY8hFNRH0H2yCebZ-JJAIkIcFfLa5GOyRTI_orCd2gvEJmDDvwkDKTd4h0csc7c_gkQy4guvUKQzo3lSvDLiE58_7WXX37evt5dXm-ub7j8sv1xvdUJE32LGaScNFw6ET0GHdoRZyKwUyg7zrQdPemK5vhh4ZMCaw3cryomNG1F3Pz6rPq--8bCccNPocwamSbIL4qAJY9a_i7U6NYa8aQRmXrBi8fzaI4WHBlNV9WKIvmVUtKReyZb0s1IeVKh-ZSlvmMKGm6qlCdaiwsO-OIx3Iv4UV4OMKpCL5EePRyP_cfgPUnKpb</recordid><startdate>20170424</startdate><enddate>20170424</enddate><creator>Khodanovich, Marina Yu</creator><creator>Sorokina, Irina V.</creator><creator>Glazacheva, Valentina Yu</creator><creator>Akulov, Andrey E.</creator><creator>Nemirovich-Danchenko, Nikolay M.</creator><creator>Romashchenko, Alexander V.</creator><creator>Tolstikova, Tatyana G.</creator><creator>Mustafina, Lilia R.</creator><creator>Yarnykh, Vasily L.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20170424</creationdate><title>Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model</title><author>Khodanovich, Marina Yu ; Sorokina, Irina V. ; Glazacheva, Valentina Yu ; Akulov, Andrey E. ; Nemirovich-Danchenko, Nikolay M. ; Romashchenko, Alexander V. ; Tolstikova, Tatyana G. ; Mustafina, Lilia R. ; Yarnykh, Vasily L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-e72129f3453a74a7e17ec49b94e2fe378ac08ff785d8e2a224e6b929f72f41783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/51</topic><topic>631/1647/245/1628</topic><topic>631/378/2606/1666</topic><topic>Animals</topic><topic>Anterior commissure</topic><topic>Biomarkers</topic><topic>Brain</topic><topic>Brain mapping</topic><topic>Corpus callosum</topic><topic>Cuprizone</topic><topic>Demyelination</topic><topic>Histology</topic><topic>Humanities and Social Sciences</topic><topic>Macromolecules</topic><topic>Magnetic resonance imaging</topic><topic>multidisciplinary</topic><topic>Multiple sclerosis</topic><topic>Myelin</topic><topic>Neuroimaging</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Regions</topic><topic>Regression analysis</topic><topic>Science</topic><topic>Substantia alba</topic><topic>Substantia grisea</topic><topic>Thalamus</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khodanovich, Marina Yu</creatorcontrib><creatorcontrib>Sorokina, Irina V.</creatorcontrib><creatorcontrib>Glazacheva, Valentina Yu</creatorcontrib><creatorcontrib>Akulov, Andrey E.</creatorcontrib><creatorcontrib>Nemirovich-Danchenko, Nikolay M.</creatorcontrib><creatorcontrib>Romashchenko, Alexander V.</creatorcontrib><creatorcontrib>Tolstikova, Tatyana G.</creatorcontrib><creatorcontrib>Mustafina, Lilia R.</creatorcontrib><creatorcontrib>Yarnykh, Vasily L.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khodanovich, Marina Yu</au><au>Sorokina, Irina V.</au><au>Glazacheva, Valentina Yu</au><au>Akulov, Andrey E.</au><au>Nemirovich-Danchenko, Nikolay M.</au><au>Romashchenko, Alexander V.</au><au>Tolstikova, Tatyana G.</au><au>Mustafina, Lilia R.</au><au>Yarnykh, Vasily L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-04-24</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>46686</spage><pages>46686-</pages><artnum>46686</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of sensitivity to both white matter (WM) and gray matter (GM) demyelination. This study aimed to histologically validate the capability of MPF mapping to quantify myelin loss in brain tissues using the cuprizone demyelination model. Whole-brain MPF maps were obtained
in vivo
on an 11.7T animal MRI scanner from 7 cuprizone-treated and 7 control С57BL/6 mice using the fast single-point synthetic-reference method. Brain sections were histologically stained with Luxol Fast Blue (LFB) for myelin quantification. Significant (p < 0.05) demyelination in cuprizone-treated animals was found according to both LFB staining and MPF in all anatomical structures (corpus callosum, anterior commissure, internal capsule, thalamus, caudoputamen, and cortex). MPF strongly correlated with quantitative histology in all animals (r = 0.95, p < 0.001) as well as in treatment and control groups taken separately (r = 0.96, p = 0.002 and r = 0.93, p = 0.007, respectively). Close agreement between histological myelin staining and MPF suggests that fast MPF mapping enables robust and accurate quantitative assessment of demyelination in both WM and GM.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28436460</pmid><doi>10.1038/srep46686</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2017-04, Vol.7 (1), p.46686, Article 46686 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5402392 |
| source | DOAJ Directory of Open Access Journals; Nature Journals Online; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 13/51 631/1647/245/1628 631/378/2606/1666 Animals Anterior commissure Biomarkers Brain Brain mapping Corpus callosum Cuprizone Demyelination Histology Humanities and Social Sciences Macromolecules Magnetic resonance imaging multidisciplinary Multiple sclerosis Myelin Neuroimaging NMR Nuclear magnetic resonance Regions Regression analysis Science Substantia alba Substantia grisea Thalamus Variance analysis |
| title | Histological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T20%3A55%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Histological%20validation%20of%20fast%20macromolecular%20proton%20fraction%20mapping%20as%20a%20quantitative%20myelin%20imaging%20method%20in%20the%20cuprizone%20demyelination%20model&rft.jtitle=Scientific%20reports&rft.au=Khodanovich,%20Marina%20Yu&rft.date=2017-04-24&rft.volume=7&rft.issue=1&rft.spage=46686&rft.pages=46686-&rft.artnum=46686&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep46686&rft_dat=%3Cproquest_pubme%3E1903496289%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1903496289&rft_id=info:pmid/28436460&rfr_iscdi=true |