Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro
Objective The aim of this study was to determine the effect of an oxygenating mouthwash compared to two other established mouthwash products on bacterial composition and metabolic activity of oral biofilms in vitro. Material and methods Twelve healthy subjects participated as donors. Plaque-saliva m...
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| Veröffentlicht in: | Clinical oral investigations 2017-05, Vol.21 (4), p.1221-1230 |
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| creator | Fernandez y Mostajo, Mercedes Exterkate, Rob A. M. Buijs, Mark J. Crielaard, Wim Zaura, Egija |
| description | Objective
The aim of this study was to determine the effect of an oxygenating mouthwash compared to two other established mouthwash products on bacterial composition and metabolic activity of oral biofilms in vitro.
Material and methods
Twelve healthy subjects participated as donors. Plaque-saliva mixture inoculated biofilms were grown and treated with 3 different chemotherapeutic mouthwashes [amine fluoride/stannous fluoride (MD), oxygenating agent (AX), chlorhexidine 0.12 % (PA), and water (W)]. Effects of treatments were assessed on biofilm composition (16S rRNA gene amplicon sequencing), production of organic acids (formate, acetate, lactate, propionate, butyrate using capillary electrophoresis), and viability of the remaining biofilm (CFUs).
Results
Microbial profiles of biofilms clustered per inoculum donor and were dominated by the genera
Veillonella
,
Streptococcus
, and
Prevotella
. Microbial diversity was only reduced after PA treatment. Significant changes in composition occurred after treatment with AX, resulting in lower proportions of
Veillonella
and higher proportions of non-mutans streptococci. Production of all organic acids after PA and lactate after MD was significantly lower as compared to W. AX resulted in reduction of acetate, butyrate, and propionate and increase in lactate production (
p
|
| doi_str_mv | 10.1007/s00784-016-1876-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5399055</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1826705618</sourcerecordid><originalsourceid>FETCH-LOGICAL-c503t-252c350432a39788359e3f50ea297cf10fb5e6be89f0301dd355635d71f111743</originalsourceid><addsrcrecordid>eNqNkU1rFTEUhoNYbK3-ADcScONmbE4y-doIUuoHFNy065DJJPemzEyuSaal_95cby1VENzkg_c57znJi9AbIB-AEHlW2qL6joDoQEnR0WfoBHomOiYlPP91pp3QCo7Ry1JuCIFeSPYCHVPJmNRSnCBzEYJ3FaeA57TW7Z0tW19wWnDdeuzSvEsl1tjudhnx7Ksd0hQdtq7G21jv94Up2wkPMYU4zQVvcrpbcFxwk3N6hY6CnYp__bCfouvPF1fnX7vL71--nX-67BwnrHaUU8c4afNapqVSjGvPAifeUi1dABIG7sXglQ6EERhHxrlgfJQQAED27BR9PPju1mH2o_NLbVOZXY6zzfcm2Wj-VJa4NZt0azjTmnDeDN4_GOT0Y_WlmjkW56fJLj6txYDSksleCvUfKBWScAF79N1f6E1a89J-olFKay2BQqPgQLmcSsk-PM4NxOyTNoekTUva7JM2tNW8ffrgx4rf0TaAHoDSpGXj85PW_3T9Cd0es6w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1889997121</pqid></control><display><type>article</type><title>Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Fernandez y Mostajo, Mercedes ; Exterkate, Rob A. M. ; Buijs, Mark J. ; Crielaard, Wim ; Zaura, Egija</creator><creatorcontrib>Fernandez y Mostajo, Mercedes ; Exterkate, Rob A. M. ; Buijs, Mark J. ; Crielaard, Wim ; Zaura, Egija</creatorcontrib><description>Objective
The aim of this study was to determine the effect of an oxygenating mouthwash compared to two other established mouthwash products on bacterial composition and metabolic activity of oral biofilms in vitro.
Material and methods
Twelve healthy subjects participated as donors. Plaque-saliva mixture inoculated biofilms were grown and treated with 3 different chemotherapeutic mouthwashes [amine fluoride/stannous fluoride (MD), oxygenating agent (AX), chlorhexidine 0.12 % (PA), and water (W)]. Effects of treatments were assessed on biofilm composition (16S rRNA gene amplicon sequencing), production of organic acids (formate, acetate, lactate, propionate, butyrate using capillary electrophoresis), and viability of the remaining biofilm (CFUs).
Results
Microbial profiles of biofilms clustered per inoculum donor and were dominated by the genera
Veillonella
,
Streptococcus
, and
Prevotella
. Microbial diversity was only reduced after PA treatment. Significant changes in composition occurred after treatment with AX, resulting in lower proportions of
Veillonella
and higher proportions of non-mutans streptococci. Production of all organic acids after PA and lactate after MD was significantly lower as compared to W. AX resulted in reduction of acetate, butyrate, and propionate and increase in lactate production (
p
< 0.05). Viable counts were significantly lower after PA and AX treatments compared to W, while no significant reduction was observed after MD.
Conclusions
All studied mouthwashes affected the in vitro biofilms differently. The effects of the AX treatment were the most prominent which resulted in changes of the bacterial composition and metabolism.
Clinical implications
Awareness by the dental team that mouthwashes can change the bacterial composition and metabolism is important when advising its use.</description><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-016-1876-2</identifier><identifier>PMID: 27337976</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adult ; Amines - pharmacology ; Anti-Infective Agents, Local - pharmacology ; Biofilms - drug effects ; Chlorhexidine - pharmacology ; Dentistry ; Drug Combinations ; Female ; Fluorides, Topical - pharmacology ; Humans ; In Vitro Techniques ; Male ; Medicine ; Metagenome ; Microbial Sensitivity Tests ; Middle Aged ; Mouthwashes - pharmacology ; Original ; Original Article ; Oxidants - pharmacology ; Prevotella ; Streptococcus ; Tin Fluorides - pharmacology ; Veillonella</subject><ispartof>Clinical oral investigations, 2017-05, Vol.21 (4), p.1221-1230</ispartof><rights>The Author(s) 2016</rights><rights>Clinical Oral Investigations is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-252c350432a39788359e3f50ea297cf10fb5e6be89f0301dd355635d71f111743</citedby><cites>FETCH-LOGICAL-c503t-252c350432a39788359e3f50ea297cf10fb5e6be89f0301dd355635d71f111743</cites><orcidid>0000-0002-5341-3015</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-016-1876-2$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-016-1876-2$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27337976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernandez y Mostajo, Mercedes</creatorcontrib><creatorcontrib>Exterkate, Rob A. M.</creatorcontrib><creatorcontrib>Buijs, Mark J.</creatorcontrib><creatorcontrib>Crielaard, Wim</creatorcontrib><creatorcontrib>Zaura, Egija</creatorcontrib><title>Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objective
The aim of this study was to determine the effect of an oxygenating mouthwash compared to two other established mouthwash products on bacterial composition and metabolic activity of oral biofilms in vitro.
Material and methods
Twelve healthy subjects participated as donors. Plaque-saliva mixture inoculated biofilms were grown and treated with 3 different chemotherapeutic mouthwashes [amine fluoride/stannous fluoride (MD), oxygenating agent (AX), chlorhexidine 0.12 % (PA), and water (W)]. Effects of treatments were assessed on biofilm composition (16S rRNA gene amplicon sequencing), production of organic acids (formate, acetate, lactate, propionate, butyrate using capillary electrophoresis), and viability of the remaining biofilm (CFUs).
Results
Microbial profiles of biofilms clustered per inoculum donor and were dominated by the genera
Veillonella
,
Streptococcus
, and
Prevotella
. Microbial diversity was only reduced after PA treatment. Significant changes in composition occurred after treatment with AX, resulting in lower proportions of
Veillonella
and higher proportions of non-mutans streptococci. Production of all organic acids after PA and lactate after MD was significantly lower as compared to W. AX resulted in reduction of acetate, butyrate, and propionate and increase in lactate production (
p
< 0.05). Viable counts were significantly lower after PA and AX treatments compared to W, while no significant reduction was observed after MD.
Conclusions
All studied mouthwashes affected the in vitro biofilms differently. The effects of the AX treatment were the most prominent which resulted in changes of the bacterial composition and metabolism.
Clinical implications
Awareness by the dental team that mouthwashes can change the bacterial composition and metabolism is important when advising its use.</description><subject>Adult</subject><subject>Amines - pharmacology</subject><subject>Anti-Infective Agents, Local - pharmacology</subject><subject>Biofilms - drug effects</subject><subject>Chlorhexidine - pharmacology</subject><subject>Dentistry</subject><subject>Drug Combinations</subject><subject>Female</subject><subject>Fluorides, Topical - pharmacology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Medicine</subject><subject>Metagenome</subject><subject>Microbial Sensitivity Tests</subject><subject>Middle Aged</subject><subject>Mouthwashes - pharmacology</subject><subject>Original</subject><subject>Original Article</subject><subject>Oxidants - pharmacology</subject><subject>Prevotella</subject><subject>Streptococcus</subject><subject>Tin Fluorides - pharmacology</subject><subject>Veillonella</subject><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1rFTEUhoNYbK3-ADcScONmbE4y-doIUuoHFNy065DJJPemzEyuSaal_95cby1VENzkg_c57znJi9AbIB-AEHlW2qL6joDoQEnR0WfoBHomOiYlPP91pp3QCo7Ry1JuCIFeSPYCHVPJmNRSnCBzEYJ3FaeA57TW7Z0tW19wWnDdeuzSvEsl1tjudhnx7Ksd0hQdtq7G21jv94Up2wkPMYU4zQVvcrpbcFxwk3N6hY6CnYp__bCfouvPF1fnX7vL71--nX-67BwnrHaUU8c4afNapqVSjGvPAifeUi1dABIG7sXglQ6EERhHxrlgfJQQAED27BR9PPju1mH2o_NLbVOZXY6zzfcm2Wj-VJa4NZt0azjTmnDeDN4_GOT0Y_WlmjkW56fJLj6txYDSksleCvUfKBWScAF79N1f6E1a89J-olFKay2BQqPgQLmcSsk-PM4NxOyTNoekTUva7JM2tNW8ffrgx4rf0TaAHoDSpGXj85PW_3T9Cd0es6w</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Fernandez y Mostajo, Mercedes</creator><creator>Exterkate, Rob A. M.</creator><creator>Buijs, Mark J.</creator><creator>Crielaard, Wim</creator><creator>Zaura, Egija</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5341-3015</orcidid></search><sort><creationdate>20170501</creationdate><title>Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro</title><author>Fernandez y Mostajo, Mercedes ; Exterkate, Rob A. M. ; Buijs, Mark J. ; Crielaard, Wim ; Zaura, Egija</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-252c350432a39788359e3f50ea297cf10fb5e6be89f0301dd355635d71f111743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Amines - pharmacology</topic><topic>Anti-Infective Agents, Local - pharmacology</topic><topic>Biofilms - drug effects</topic><topic>Chlorhexidine - pharmacology</topic><topic>Dentistry</topic><topic>Drug Combinations</topic><topic>Female</topic><topic>Fluorides, Topical - pharmacology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Medicine</topic><topic>Metagenome</topic><topic>Microbial Sensitivity Tests</topic><topic>Middle Aged</topic><topic>Mouthwashes - pharmacology</topic><topic>Original</topic><topic>Original Article</topic><topic>Oxidants - pharmacology</topic><topic>Prevotella</topic><topic>Streptococcus</topic><topic>Tin Fluorides - pharmacology</topic><topic>Veillonella</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernandez y Mostajo, Mercedes</creatorcontrib><creatorcontrib>Exterkate, Rob A. M.</creatorcontrib><creatorcontrib>Buijs, Mark J.</creatorcontrib><creatorcontrib>Crielaard, Wim</creatorcontrib><creatorcontrib>Zaura, Egija</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernandez y Mostajo, Mercedes</au><au>Exterkate, Rob A. M.</au><au>Buijs, Mark J.</au><au>Crielaard, Wim</au><au>Zaura, Egija</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>21</volume><issue>4</issue><spage>1221</spage><epage>1230</epage><pages>1221-1230</pages><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objective
The aim of this study was to determine the effect of an oxygenating mouthwash compared to two other established mouthwash products on bacterial composition and metabolic activity of oral biofilms in vitro.
Material and methods
Twelve healthy subjects participated as donors. Plaque-saliva mixture inoculated biofilms were grown and treated with 3 different chemotherapeutic mouthwashes [amine fluoride/stannous fluoride (MD), oxygenating agent (AX), chlorhexidine 0.12 % (PA), and water (W)]. Effects of treatments were assessed on biofilm composition (16S rRNA gene amplicon sequencing), production of organic acids (formate, acetate, lactate, propionate, butyrate using capillary electrophoresis), and viability of the remaining biofilm (CFUs).
Results
Microbial profiles of biofilms clustered per inoculum donor and were dominated by the genera
Veillonella
,
Streptococcus
, and
Prevotella
. Microbial diversity was only reduced after PA treatment. Significant changes in composition occurred after treatment with AX, resulting in lower proportions of
Veillonella
and higher proportions of non-mutans streptococci. Production of all organic acids after PA and lactate after MD was significantly lower as compared to W. AX resulted in reduction of acetate, butyrate, and propionate and increase in lactate production (
p
< 0.05). Viable counts were significantly lower after PA and AX treatments compared to W, while no significant reduction was observed after MD.
Conclusions
All studied mouthwashes affected the in vitro biofilms differently. The effects of the AX treatment were the most prominent which resulted in changes of the bacterial composition and metabolism.
Clinical implications
Awareness by the dental team that mouthwashes can change the bacterial composition and metabolism is important when advising its use.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27337976</pmid><doi>10.1007/s00784-016-1876-2</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-5341-3015</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1432-6981 |
| ispartof | Clinical oral investigations, 2017-05, Vol.21 (4), p.1221-1230 |
| issn | 1432-6981 1436-3771 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Adult Amines - pharmacology Anti-Infective Agents, Local - pharmacology Biofilms - drug effects Chlorhexidine - pharmacology Dentistry Drug Combinations Female Fluorides, Topical - pharmacology Humans In Vitro Techniques Male Medicine Metagenome Microbial Sensitivity Tests Middle Aged Mouthwashes - pharmacology Original Original Article Oxidants - pharmacology Prevotella Streptococcus Tin Fluorides - pharmacology Veillonella |
| title | Effect of mouthwashes on the composition and metabolic activity of oral biofilms grown in vitro |
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