Persimmon Leaves (Diospyros kaki) Extract Protects Optic Nerve Crush-Induced Retinal Degeneration
Retinal ganglion cell (RGC) death is part of many retinal diseases. Here, we report that the ethanol extract of Diospyros kaki (EEDK) exhibits protective properties against retinal degeneration, both in vitro and in vivo . Upon exposure to cytotoxic compounds, RGC-5 cells showed approximately 40% ce...
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| Veröffentlicht in: | Scientific reports 2017-04, Vol.7 (1), p.46449-46449, Article 46449 |
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| creator | Ryul Ahn, Hong Kim, Kyung-A Kang, Suk Woo Lee, Joo Young Kim, Tae-Jin Jung, Sang Hoon |
| description | Retinal ganglion cell (RGC) death is part of many retinal diseases. Here, we report that the ethanol extract of
Diospyros kaki
(EEDK) exhibits protective properties against retinal degeneration, both
in vitro
and
in vivo
. Upon exposure to cytotoxic compounds, RGC-5 cells showed approximately 40% cell viability versus the control, while pre-treatment with EEDK markedly increased cell viability in a concentration-dependent manner. Further studies revealed that cell survival induced by EEDK was associated with decreased levels of apoptotic proteins, such as poly (ADP-ribose) polymerase, p53, and cleaved caspase-3. In addition to apoptotic pathways, we demonstrated that expression levels of antioxidant-associated proteins, such as superoxide dismutase-1, glutathione S-transferase, and glutathione peroxidase-1, were positively modulated by EEDK. In a partial optic nerve crush mouse model, EEDK had similar ameliorating effects on retinal degeneration resulting from mechanical damages. Therefore, our results suggest that EEDK may have therapeutic potential against retinal degenerative disorders, such as glaucoma. |
| doi_str_mv | 10.1038/srep46449 |
| format | Article |
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Diospyros kaki
(EEDK) exhibits protective properties against retinal degeneration, both
in vitro
and
in vivo
. Upon exposure to cytotoxic compounds, RGC-5 cells showed approximately 40% cell viability versus the control, while pre-treatment with EEDK markedly increased cell viability in a concentration-dependent manner. Further studies revealed that cell survival induced by EEDK was associated with decreased levels of apoptotic proteins, such as poly (ADP-ribose) polymerase, p53, and cleaved caspase-3. In addition to apoptotic pathways, we demonstrated that expression levels of antioxidant-associated proteins, such as superoxide dismutase-1, glutathione S-transferase, and glutathione peroxidase-1, were positively modulated by EEDK. In a partial optic nerve crush mouse model, EEDK had similar ameliorating effects on retinal degeneration resulting from mechanical damages. Therefore, our results suggest that EEDK may have therapeutic potential against retinal degenerative disorders, such as glaucoma.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep46449</identifier><identifier>PMID: 28425487</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/2 ; 13/51 ; 631/378/1934 ; 631/80/82/23 ; 96/63 ; Adenosine diphosphate ; Apoptosis ; Caspase ; Caspase-3 ; Cell death ; Cell survival ; Cytotoxicity ; Diospyros kaki ; Ethanol ; Glaucoma ; Glutathione peroxidase ; Glutathione transferase ; Humanities and Social Sciences ; multidisciplinary ; Optic nerve ; p53 Protein ; Retina ; Retinal degeneration ; Retinal ganglion cells ; Ribose ; Rodents ; Science ; Superoxide dismutase</subject><ispartof>Scientific reports, 2017-04, Vol.7 (1), p.46449-46449, Article 46449</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Apr 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-d8f6bcfa3781d56ec93e93a777111a2cd1f951851915bb5e054837fceec867533</citedby><cites>FETCH-LOGICAL-c504t-d8f6bcfa3781d56ec93e93a777111a2cd1f951851915bb5e054837fceec867533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397840/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397840/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28425487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ryul Ahn, Hong</creatorcontrib><creatorcontrib>Kim, Kyung-A</creatorcontrib><creatorcontrib>Kang, Suk Woo</creatorcontrib><creatorcontrib>Lee, Joo Young</creatorcontrib><creatorcontrib>Kim, Tae-Jin</creatorcontrib><creatorcontrib>Jung, Sang Hoon</creatorcontrib><title>Persimmon Leaves (Diospyros kaki) Extract Protects Optic Nerve Crush-Induced Retinal Degeneration</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Retinal ganglion cell (RGC) death is part of many retinal diseases. Here, we report that the ethanol extract of
Diospyros kaki
(EEDK) exhibits protective properties against retinal degeneration, both
in vitro
and
in vivo
. Upon exposure to cytotoxic compounds, RGC-5 cells showed approximately 40% cell viability versus the control, while pre-treatment with EEDK markedly increased cell viability in a concentration-dependent manner. Further studies revealed that cell survival induced by EEDK was associated with decreased levels of apoptotic proteins, such as poly (ADP-ribose) polymerase, p53, and cleaved caspase-3. In addition to apoptotic pathways, we demonstrated that expression levels of antioxidant-associated proteins, such as superoxide dismutase-1, glutathione S-transferase, and glutathione peroxidase-1, were positively modulated by EEDK. In a partial optic nerve crush mouse model, EEDK had similar ameliorating effects on retinal degeneration resulting from mechanical damages. Therefore, our results suggest that EEDK may have therapeutic potential against retinal degenerative disorders, such as glaucoma.</description><subject>13/2</subject><subject>13/51</subject><subject>631/378/1934</subject><subject>631/80/82/23</subject><subject>96/63</subject><subject>Adenosine diphosphate</subject><subject>Apoptosis</subject><subject>Caspase</subject><subject>Caspase-3</subject><subject>Cell death</subject><subject>Cell survival</subject><subject>Cytotoxicity</subject><subject>Diospyros kaki</subject><subject>Ethanol</subject><subject>Glaucoma</subject><subject>Glutathione peroxidase</subject><subject>Glutathione transferase</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Optic nerve</subject><subject>p53 Protein</subject><subject>Retina</subject><subject>Retinal degeneration</subject><subject>Retinal ganglion cells</subject><subject>Ribose</subject><subject>Rodents</subject><subject>Science</subject><subject>Superoxide dismutase</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkV9rFDEUxYMottQ--AUk4EsrjObvTvIiyLZqYbFF9DlkM3e2aWeSMcks9tsb2bqsel9y4fw4OclB6CUlbynh6l1OMImFEPoJOmZEyIZxxp4e7EfoNOc7UkcyLah-jo6YEkwK1R4jewMp-3GMAa_AbiHjswsf8_SQYsb39t6f48ufJVlX8E2KBVzJ-Hoq3uEvkLaAl2nOt81V6GYHHf4KxQc74AvYQIBki4_hBXrW2yHD6eN5gr5_vPy2_Nysrj9dLT-sGieJKE2n-sXa9Za3inZyAU5z0Ny2bUsptcx1tNeSKkk1leu1BFLz87Z3AE4tWsn5CXq_853m9Qidg1BjD2ZKfrTpwUTrzd9K8LdmE7dGct0qQarB2aNBij9myMWMPjsYBhsgztlQpSlRmpG2oq__Qe_inOrLK6UJF0IKoip1vqNc_cxaU78PQ4n53Z3Zd1fZV4fp9-SfpirwZgfkKoUNpIMr_3P7BfvEo6I</recordid><startdate>20170420</startdate><enddate>20170420</enddate><creator>Ryul Ahn, Hong</creator><creator>Kim, Kyung-A</creator><creator>Kang, Suk Woo</creator><creator>Lee, Joo Young</creator><creator>Kim, Tae-Jin</creator><creator>Jung, Sang Hoon</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170420</creationdate><title>Persimmon Leaves (Diospyros kaki) Extract Protects Optic Nerve Crush-Induced Retinal Degeneration</title><author>Ryul Ahn, Hong ; Kim, Kyung-A ; Kang, Suk Woo ; Lee, Joo Young ; Kim, Tae-Jin ; Jung, Sang Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-d8f6bcfa3781d56ec93e93a777111a2cd1f951851915bb5e054837fceec867533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/2</topic><topic>13/51</topic><topic>631/378/1934</topic><topic>631/80/82/23</topic><topic>96/63</topic><topic>Adenosine diphosphate</topic><topic>Apoptosis</topic><topic>Caspase</topic><topic>Caspase-3</topic><topic>Cell death</topic><topic>Cell survival</topic><topic>Cytotoxicity</topic><topic>Diospyros kaki</topic><topic>Ethanol</topic><topic>Glaucoma</topic><topic>Glutathione peroxidase</topic><topic>Glutathione transferase</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Optic nerve</topic><topic>p53 Protein</topic><topic>Retina</topic><topic>Retinal degeneration</topic><topic>Retinal ganglion cells</topic><topic>Ribose</topic><topic>Rodents</topic><topic>Science</topic><topic>Superoxide dismutase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ryul Ahn, Hong</creatorcontrib><creatorcontrib>Kim, Kyung-A</creatorcontrib><creatorcontrib>Kang, Suk Woo</creatorcontrib><creatorcontrib>Lee, Joo Young</creatorcontrib><creatorcontrib>Kim, Tae-Jin</creatorcontrib><creatorcontrib>Jung, Sang Hoon</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ryul Ahn, Hong</au><au>Kim, Kyung-A</au><au>Kang, Suk Woo</au><au>Lee, Joo Young</au><au>Kim, Tae-Jin</au><au>Jung, Sang Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Persimmon Leaves (Diospyros kaki) Extract Protects Optic Nerve Crush-Induced Retinal Degeneration</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-04-20</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>46449</spage><epage>46449</epage><pages>46449-46449</pages><artnum>46449</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Retinal ganglion cell (RGC) death is part of many retinal diseases. Here, we report that the ethanol extract of
Diospyros kaki
(EEDK) exhibits protective properties against retinal degeneration, both
in vitro
and
in vivo
. Upon exposure to cytotoxic compounds, RGC-5 cells showed approximately 40% cell viability versus the control, while pre-treatment with EEDK markedly increased cell viability in a concentration-dependent manner. Further studies revealed that cell survival induced by EEDK was associated with decreased levels of apoptotic proteins, such as poly (ADP-ribose) polymerase, p53, and cleaved caspase-3. In addition to apoptotic pathways, we demonstrated that expression levels of antioxidant-associated proteins, such as superoxide dismutase-1, glutathione S-transferase, and glutathione peroxidase-1, were positively modulated by EEDK. In a partial optic nerve crush mouse model, EEDK had similar ameliorating effects on retinal degeneration resulting from mechanical damages. Therefore, our results suggest that EEDK may have therapeutic potential against retinal degenerative disorders, such as glaucoma.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28425487</pmid><doi>10.1038/srep46449</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/2 13/51 631/378/1934 631/80/82/23 96/63 Adenosine diphosphate Apoptosis Caspase Caspase-3 Cell death Cell survival Cytotoxicity Diospyros kaki Ethanol Glaucoma Glutathione peroxidase Glutathione transferase Humanities and Social Sciences multidisciplinary Optic nerve p53 Protein Retina Retinal degeneration Retinal ganglion cells Ribose Rodents Science Superoxide dismutase |
| title | Persimmon Leaves (Diospyros kaki) Extract Protects Optic Nerve Crush-Induced Retinal Degeneration |
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