Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities
Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragon’s blood. Excessive utilization of the plant for extraction of dragon’s blood had resulted in the destruction of the important niche. During a study to provide a sustainable way...
Gespeichert in:
| Veröffentlicht in: | BioMed research international 2017-01, Vol.2017 (2017), p.1-11 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 11 |
|---|---|
| container_issue | 2017 |
| container_start_page | 1 |
| container_title | BioMed research international |
| container_volume | 2017 |
| creator | Sarter, Samira Li, Wen-Jun Fontana, Angélique Phi, Quyet-Tien Quach, Ngoc-Tung Chu-Ky, Son Vu, Thu-Trang Liu, Min-Jiao Khieu, Thi-Nhan Salam, Nimaichand Manikprabhu, Deene |
| description | Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragon’s blood. Excessive utilization of the plant for extraction of dragon’s blood had resulted in the destruction of the important niche. During a study to provide a sustainable way of utilizing the resources, the endophytic Actinobacteria associated with the plant were explored for potential utilization of their medicinal properties. Three hundred and four endophytic Actinobacteria belonging to the genera Streptomyces, Nocardiopsis, Brevibacterium, Microbacterium, Tsukamurella, Arthrobacter, Brachybacterium, Nocardia, Rhodococcus, Kocuria, Nocardioides, and Pseudonocardia were isolated from different tissues of D. cochinchinensis Lour. Of these, 17 strains having antimicrobial and anthracyclines-producing activities were further selected for screening of antifungal and cytotoxic activities against two human cancer cell lines, MCF-7 and Hep G2. Ten of these selected endophytic Actinobacteria showed antifungal activities against at least one of the fungal pathogens, of which three strains exhibited cytotoxic activities with IC50-values ranging between 3 and 33 μg·mL−1. Frequencies for the presence of biosynthetic genes, polyketide synthase- (PKS-) I, PKS-II, and nonribosomal peptide synthetase (NRPS) among these 17 selected bioactive Actinobacteria were 29.4%, 70.6%, and 23.5%, respectively. The results indicated that the medicinal plant D. cochinchinensis Lour. is a good niche of biologically important metabolites-producing Actinobacteria. |
| doi_str_mv | 10.1155/2017/1308563 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5397652</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A557301270</galeid><sourcerecordid>A557301270</sourcerecordid><originalsourceid>FETCH-LOGICAL-c566t-ea3ddf816855ff723bd684966e5330ce30932e068f556458b464706c287574c3</originalsourceid><addsrcrecordid>eNqNks1r2zAYxs3YWEvX285DsMvGklbflnsYmLRbC4FdcheK_LpWcaTMUtLlsr99MsnSracKhIT00_PqfXiK4j3BF4QIcUkxKS8Jw0pI9qo4pYzwqSScvD7uGTspzmN8wHkoInEl3xYnVHHFSyxPi983vgnrbpecRbVNzoelsQkGZ1AdY7DOJGjQo0sduh6MNeANssF2zo8TfHQRzcNmuLhCdzH0JrngJ-jabWGILu0myPgGLTpwA5rtUkjh16HQ1iUH8V3xpjV9hPPDelYsvt0sZrfT-Y_vd7N6PrVCyjQFw5qmzd9XQrRtSdmykYpXUoJgDFtguGIUsFStEJILteRybM9SVYqSW3ZWfN3LrjfLFTQWfBpMr9eDW5lhp4Nx-v8b7zp9H7ZasKqUgmaBz3uB7tmz23quxzNMKlYRorYks58OxYbwcwMx6ZWLFvreeAibqImqSlZRTNlLUDnSnGf04zP0Idvus2mZUhWTnFP5RN2bHrTzbcjt2FFU10KUDBNa4kxN9pQdQowDtMeWCNZjrvSYK33IVcY__OveEf6bogx8ObjjfGMe3QvlIDPQmic6h1UxzP4AeR3czQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1889364426</pqid></control><display><type>article</type><title>Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>Wiley Online Library Open Access</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Sarter, Samira ; Li, Wen-Jun ; Fontana, Angélique ; Phi, Quyet-Tien ; Quach, Ngoc-Tung ; Chu-Ky, Son ; Vu, Thu-Trang ; Liu, Min-Jiao ; Khieu, Thi-Nhan ; Salam, Nimaichand ; Manikprabhu, Deene</creator><contributor>Lee, Young-Mi</contributor><creatorcontrib>Sarter, Samira ; Li, Wen-Jun ; Fontana, Angélique ; Phi, Quyet-Tien ; Quach, Ngoc-Tung ; Chu-Ky, Son ; Vu, Thu-Trang ; Liu, Min-Jiao ; Khieu, Thi-Nhan ; Salam, Nimaichand ; Manikprabhu, Deene ; Lee, Young-Mi</creatorcontrib><description>Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragon’s blood. Excessive utilization of the plant for extraction of dragon’s blood had resulted in the destruction of the important niche. During a study to provide a sustainable way of utilizing the resources, the endophytic Actinobacteria associated with the plant were explored for potential utilization of their medicinal properties. Three hundred and four endophytic Actinobacteria belonging to the genera Streptomyces, Nocardiopsis, Brevibacterium, Microbacterium, Tsukamurella, Arthrobacter, Brachybacterium, Nocardia, Rhodococcus, Kocuria, Nocardioides, and Pseudonocardia were isolated from different tissues of D. cochinchinensis Lour. Of these, 17 strains having antimicrobial and anthracyclines-producing activities were further selected for screening of antifungal and cytotoxic activities against two human cancer cell lines, MCF-7 and Hep G2. Ten of these selected endophytic Actinobacteria showed antifungal activities against at least one of the fungal pathogens, of which three strains exhibited cytotoxic activities with IC50-values ranging between 3 and 33 μg·mL−1. Frequencies for the presence of biosynthetic genes, polyketide synthase- (PKS-) I, PKS-II, and nonribosomal peptide synthetase (NRPS) among these 17 selected bioactive Actinobacteria were 29.4%, 70.6%, and 23.5%, respectively. The results indicated that the medicinal plant D. cochinchinensis Lour. is a good niche of biologically important metabolites-producing Actinobacteria.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2017/1308563</identifier><identifier>PMID: 28484706</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Actinobacteria ; Actinobacteria - classification ; Actinobacteria - isolation & purification ; Actinobacteria - metabolism ; Actinomycetes ; Antibiotics ; Antifungal Agents - metabolism ; Antifungal Agents - pharmacology ; Antineoplastic Agents - metabolism ; Antineoplastic Agents - pharmacology ; Arthrobacter ; Brachybacterium ; Brevibacterium ; Chemical properties ; Cytotoxicity ; Cytotoxins - biosynthesis ; Cytotoxins - pharmacology ; Deoxyribonucleic acid ; Distribution ; DNA ; Dracaena ; Dracaena - microbiology ; Ethanol ; Extraction (Chemistry) ; Food science ; Genes ; Genomics ; Health aspects ; Hep G2 Cells ; Herbal medicine ; Humans ; Laboratories ; Life Sciences ; MCF-7 Cells ; Metabolites ; Methods ; Microbacterium ; National parks ; Nocardia ; Nocardioides ; Nocardiopsis ; Pathogens ; Pseudonocardia ; Rhodococcus ; Streptomyces ; Studies</subject><ispartof>BioMed research international, 2017-01, Vol.2017 (2017), p.1-11</ispartof><rights>Copyright © 2017 Nimaichand Salam et al.</rights><rights>COPYRIGHT 2017 John Wiley & Sons, Inc.</rights><rights>Copyright © 2017 Nimaichand Salam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2017 Nimaichand Salam et al. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-ea3ddf816855ff723bd684966e5330ce30932e068f556458b464706c287574c3</citedby><cites>FETCH-LOGICAL-c566t-ea3ddf816855ff723bd684966e5330ce30932e068f556458b464706c287574c3</cites><orcidid>0000-0002-4777-3452 ; 0000-0001-6574-1326 ; 0000-0002-1233-736X ; 0000-0001-5115-0824</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397652/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397652/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28484706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01939118$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Lee, Young-Mi</contributor><creatorcontrib>Sarter, Samira</creatorcontrib><creatorcontrib>Li, Wen-Jun</creatorcontrib><creatorcontrib>Fontana, Angélique</creatorcontrib><creatorcontrib>Phi, Quyet-Tien</creatorcontrib><creatorcontrib>Quach, Ngoc-Tung</creatorcontrib><creatorcontrib>Chu-Ky, Son</creatorcontrib><creatorcontrib>Vu, Thu-Trang</creatorcontrib><creatorcontrib>Liu, Min-Jiao</creatorcontrib><creatorcontrib>Khieu, Thi-Nhan</creatorcontrib><creatorcontrib>Salam, Nimaichand</creatorcontrib><creatorcontrib>Manikprabhu, Deene</creatorcontrib><title>Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragon’s blood. Excessive utilization of the plant for extraction of dragon’s blood had resulted in the destruction of the important niche. During a study to provide a sustainable way of utilizing the resources, the endophytic Actinobacteria associated with the plant were explored for potential utilization of their medicinal properties. Three hundred and four endophytic Actinobacteria belonging to the genera Streptomyces, Nocardiopsis, Brevibacterium, Microbacterium, Tsukamurella, Arthrobacter, Brachybacterium, Nocardia, Rhodococcus, Kocuria, Nocardioides, and Pseudonocardia were isolated from different tissues of D. cochinchinensis Lour. Of these, 17 strains having antimicrobial and anthracyclines-producing activities were further selected for screening of antifungal and cytotoxic activities against two human cancer cell lines, MCF-7 and Hep G2. Ten of these selected endophytic Actinobacteria showed antifungal activities against at least one of the fungal pathogens, of which three strains exhibited cytotoxic activities with IC50-values ranging between 3 and 33 μg·mL−1. Frequencies for the presence of biosynthetic genes, polyketide synthase- (PKS-) I, PKS-II, and nonribosomal peptide synthetase (NRPS) among these 17 selected bioactive Actinobacteria were 29.4%, 70.6%, and 23.5%, respectively. The results indicated that the medicinal plant D. cochinchinensis Lour. is a good niche of biologically important metabolites-producing Actinobacteria.</description><subject>Actinobacteria</subject><subject>Actinobacteria - classification</subject><subject>Actinobacteria - isolation & purification</subject><subject>Actinobacteria - metabolism</subject><subject>Actinomycetes</subject><subject>Antibiotics</subject><subject>Antifungal Agents - metabolism</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antineoplastic Agents - metabolism</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Arthrobacter</subject><subject>Brachybacterium</subject><subject>Brevibacterium</subject><subject>Chemical properties</subject><subject>Cytotoxicity</subject><subject>Cytotoxins - biosynthesis</subject><subject>Cytotoxins - pharmacology</subject><subject>Deoxyribonucleic acid</subject><subject>Distribution</subject><subject>DNA</subject><subject>Dracaena</subject><subject>Dracaena - microbiology</subject><subject>Ethanol</subject><subject>Extraction (Chemistry)</subject><subject>Food science</subject><subject>Genes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Hep G2 Cells</subject><subject>Herbal medicine</subject><subject>Humans</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>MCF-7 Cells</subject><subject>Metabolites</subject><subject>Methods</subject><subject>Microbacterium</subject><subject>National parks</subject><subject>Nocardia</subject><subject>Nocardioides</subject><subject>Nocardiopsis</subject><subject>Pathogens</subject><subject>Pseudonocardia</subject><subject>Rhodococcus</subject><subject>Streptomyces</subject><subject>Studies</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks1r2zAYxs3YWEvX285DsMvGklbflnsYmLRbC4FdcheK_LpWcaTMUtLlsr99MsnSracKhIT00_PqfXiK4j3BF4QIcUkxKS8Jw0pI9qo4pYzwqSScvD7uGTspzmN8wHkoInEl3xYnVHHFSyxPi983vgnrbpecRbVNzoelsQkGZ1AdY7DOJGjQo0sduh6MNeANssF2zo8TfHQRzcNmuLhCdzH0JrngJ-jabWGILu0myPgGLTpwA5rtUkjh16HQ1iUH8V3xpjV9hPPDelYsvt0sZrfT-Y_vd7N6PrVCyjQFw5qmzd9XQrRtSdmykYpXUoJgDFtguGIUsFStEJILteRybM9SVYqSW3ZWfN3LrjfLFTQWfBpMr9eDW5lhp4Nx-v8b7zp9H7ZasKqUgmaBz3uB7tmz23quxzNMKlYRorYks58OxYbwcwMx6ZWLFvreeAibqImqSlZRTNlLUDnSnGf04zP0Idvus2mZUhWTnFP5RN2bHrTzbcjt2FFU10KUDBNa4kxN9pQdQowDtMeWCNZjrvSYK33IVcY__OveEf6bogx8ObjjfGMe3QvlIDPQmic6h1UxzP4AeR3czQ</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Sarter, Samira</creator><creator>Li, Wen-Jun</creator><creator>Fontana, Angélique</creator><creator>Phi, Quyet-Tien</creator><creator>Quach, Ngoc-Tung</creator><creator>Chu-Ky, Son</creator><creator>Vu, Thu-Trang</creator><creator>Liu, Min-Jiao</creator><creator>Khieu, Thi-Nhan</creator><creator>Salam, Nimaichand</creator><creator>Manikprabhu, Deene</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-4777-3452</orcidid><orcidid>https://orcid.org/0000-0001-6574-1326</orcidid><orcidid>https://orcid.org/0000-0002-1233-736X</orcidid><orcidid>https://orcid.org/0000-0001-5115-0824</orcidid></search><sort><creationdate>20170101</creationdate><title>Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities</title><author>Sarter, Samira ; Li, Wen-Jun ; Fontana, Angélique ; Phi, Quyet-Tien ; Quach, Ngoc-Tung ; Chu-Ky, Son ; Vu, Thu-Trang ; Liu, Min-Jiao ; Khieu, Thi-Nhan ; Salam, Nimaichand ; Manikprabhu, Deene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-ea3ddf816855ff723bd684966e5330ce30932e068f556458b464706c287574c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Actinobacteria</topic><topic>Actinobacteria - classification</topic><topic>Actinobacteria - isolation & purification</topic><topic>Actinobacteria - metabolism</topic><topic>Actinomycetes</topic><topic>Antibiotics</topic><topic>Antifungal Agents - metabolism</topic><topic>Antifungal Agents - pharmacology</topic><topic>Antineoplastic Agents - metabolism</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Arthrobacter</topic><topic>Brachybacterium</topic><topic>Brevibacterium</topic><topic>Chemical properties</topic><topic>Cytotoxicity</topic><topic>Cytotoxins - biosynthesis</topic><topic>Cytotoxins - pharmacology</topic><topic>Deoxyribonucleic acid</topic><topic>Distribution</topic><topic>DNA</topic><topic>Dracaena</topic><topic>Dracaena - microbiology</topic><topic>Ethanol</topic><topic>Extraction (Chemistry)</topic><topic>Food science</topic><topic>Genes</topic><topic>Genomics</topic><topic>Health aspects</topic><topic>Hep G2 Cells</topic><topic>Herbal medicine</topic><topic>Humans</topic><topic>Laboratories</topic><topic>Life Sciences</topic><topic>MCF-7 Cells</topic><topic>Metabolites</topic><topic>Methods</topic><topic>Microbacterium</topic><topic>National parks</topic><topic>Nocardia</topic><topic>Nocardioides</topic><topic>Nocardiopsis</topic><topic>Pathogens</topic><topic>Pseudonocardia</topic><topic>Rhodococcus</topic><topic>Streptomyces</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarter, Samira</creatorcontrib><creatorcontrib>Li, Wen-Jun</creatorcontrib><creatorcontrib>Fontana, Angélique</creatorcontrib><creatorcontrib>Phi, Quyet-Tien</creatorcontrib><creatorcontrib>Quach, Ngoc-Tung</creatorcontrib><creatorcontrib>Chu-Ky, Son</creatorcontrib><creatorcontrib>Vu, Thu-Trang</creatorcontrib><creatorcontrib>Liu, Min-Jiao</creatorcontrib><creatorcontrib>Khieu, Thi-Nhan</creatorcontrib><creatorcontrib>Salam, Nimaichand</creatorcontrib><creatorcontrib>Manikprabhu, Deene</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarter, Samira</au><au>Li, Wen-Jun</au><au>Fontana, Angélique</au><au>Phi, Quyet-Tien</au><au>Quach, Ngoc-Tung</au><au>Chu-Ky, Son</au><au>Vu, Thu-Trang</au><au>Liu, Min-Jiao</au><au>Khieu, Thi-Nhan</au><au>Salam, Nimaichand</au><au>Manikprabhu, Deene</au><au>Lee, Young-Mi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>2017</volume><issue>2017</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Dracaena cochinchinensis Lour. is an ethnomedicinally important plant used in traditional Chinese medicine known as dragon’s blood. Excessive utilization of the plant for extraction of dragon’s blood had resulted in the destruction of the important niche. During a study to provide a sustainable way of utilizing the resources, the endophytic Actinobacteria associated with the plant were explored for potential utilization of their medicinal properties. Three hundred and four endophytic Actinobacteria belonging to the genera Streptomyces, Nocardiopsis, Brevibacterium, Microbacterium, Tsukamurella, Arthrobacter, Brachybacterium, Nocardia, Rhodococcus, Kocuria, Nocardioides, and Pseudonocardia were isolated from different tissues of D. cochinchinensis Lour. Of these, 17 strains having antimicrobial and anthracyclines-producing activities were further selected for screening of antifungal and cytotoxic activities against two human cancer cell lines, MCF-7 and Hep G2. Ten of these selected endophytic Actinobacteria showed antifungal activities against at least one of the fungal pathogens, of which three strains exhibited cytotoxic activities with IC50-values ranging between 3 and 33 μg·mL−1. Frequencies for the presence of biosynthetic genes, polyketide synthase- (PKS-) I, PKS-II, and nonribosomal peptide synthetase (NRPS) among these 17 selected bioactive Actinobacteria were 29.4%, 70.6%, and 23.5%, respectively. The results indicated that the medicinal plant D. cochinchinensis Lour. is a good niche of biologically important metabolites-producing Actinobacteria.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>28484706</pmid><doi>10.1155/2017/1308563</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4777-3452</orcidid><orcidid>https://orcid.org/0000-0001-6574-1326</orcidid><orcidid>https://orcid.org/0000-0002-1233-736X</orcidid><orcidid>https://orcid.org/0000-0001-5115-0824</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2314-6133 |
| ispartof | BioMed research international, 2017-01, Vol.2017 (2017), p.1-11 |
| issn | 2314-6133 2314-6141 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5397652 |
| source | MEDLINE; PubMed Central Open Access; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection |
| subjects | Actinobacteria Actinobacteria - classification Actinobacteria - isolation & purification Actinobacteria - metabolism Actinomycetes Antibiotics Antifungal Agents - metabolism Antifungal Agents - pharmacology Antineoplastic Agents - metabolism Antineoplastic Agents - pharmacology Arthrobacter Brachybacterium Brevibacterium Chemical properties Cytotoxicity Cytotoxins - biosynthesis Cytotoxins - pharmacology Deoxyribonucleic acid Distribution DNA Dracaena Dracaena - microbiology Ethanol Extraction (Chemistry) Food science Genes Genomics Health aspects Hep G2 Cells Herbal medicine Humans Laboratories Life Sciences MCF-7 Cells Metabolites Methods Microbacterium National parks Nocardia Nocardioides Nocardiopsis Pathogens Pseudonocardia Rhodococcus Streptomyces Studies |
| title | Endophytic Actinobacteria Associated with Dracaena cochinchinensis Lour.: Isolation, Diversity, and Their Cytotoxic Activities |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T20%3A35%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Endophytic%20Actinobacteria%20Associated%20with%20Dracaena%20cochinchinensis%20Lour.:%20Isolation,%20Diversity,%20and%20Their%20Cytotoxic%20Activities&rft.jtitle=BioMed%20research%20international&rft.au=Sarter,%20Samira&rft.date=2017-01-01&rft.volume=2017&rft.issue=2017&rft.spage=1&rft.epage=11&rft.pages=1-11&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2017/1308563&rft_dat=%3Cgale_pubme%3EA557301270%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1889364426&rft_id=info:pmid/28484706&rft_galeid=A557301270&rfr_iscdi=true |