$Loss-less Nano-fractionator for High Sensitivity, High Coverage Proteomics$

Loss-less Nano-fractionator for High Sensitivity, High Coverage Proteomics

Recent advances in mass spectrometry (MS)-based proteomics now allow very deep coverage of cellular proteomes. To achieve near-comprehensive identification and quantification, the combination of a first HPLC-based peptide fractionation orthogonal to the on-line LC-MS/MS step has proven to be particu...

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Veröffentlicht in:	Molecular & cellular proteomics 2017-04, Vol.16 (4), p.694-705
Hauptverfasser:	Kulak, Nils A., Geyer, Philipp E., Mann, Matthias
Format:	Artikel
Sprache:	eng
Schlagworte:	Araneae Cell lines Chemical Fractionation - instrumentation Chemical Fractionation - methods Chromatography, High Pressure Liquid Flow channels Fractionation HeLa Cells High performance liquid chromatography Humans Liquid chromatography Mass spectrometry Mass spectroscopy Peptides - chemistry Proteins Proteomics Proteomics - instrumentation Proteomics - methods Sensitivity Switches Tandem Mass Spectrometry Technological Innovation and Resources
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