Preparation, Characterization and Cytotoxic Effects of Pegylated Nanoliposomal Containing Carboplatin on Ovarian Cancer Cell Lines
Carboplatin is a chemotherapeutic agent used against various malignancies such as ovarian carcinoma. The aim of this study is to improve the therapeutic efficacy of carboplatin using pegylated liposomal nanocarriers. Nanoparticles were synthesized using thin film hydration technique and characterize...
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| Veröffentlicht in: | Indian journal of clinical biochemistry 2017-06, Vol.32 (2), p.230-234 |
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| creator | Ebrahimifar, Meysam Nili-Ahmadabadi, Amir Akbarzadeh, Azim Shahemabadi, Hasan Ebrahimi Hasanzadegan, Majid Moradi-Sardareh, Hemen Madadizadeh, Hassan Rezaee-diyan, Jalal |
| description | Carboplatin is a chemotherapeutic agent used against various malignancies such as ovarian carcinoma. The aim of this study is to improve the therapeutic efficacy of carboplatin using pegylated liposomal nanocarriers. Nanoparticles were synthesized using thin film hydration technique and characterized for shape morphology, particle size, zeta potential and drug-release properties. In the next step, A2780S and A2780CP ovarian cancer cell lines were used to determine the efficacy of nanodrug by MTT assay. The particle size and zeta potential of nanodrug were measured 244.3 ± 19.6 nm and −22.9 ± 1.7 mV, respectively. High encapsulation capacity (78.6 ± 3.7 %) confirmed the efficiency of technique. The cytotoxicity results also showed that nanodrug compared to free drug improve the efficacy of carboplatin against both A2780S (
P
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| doi_str_mv | 10.1007/s12291-016-0596-3 |
| format | Article |
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P
< 0.01) and A2780CP (
P
< 0.05) cell lines. In conclusion, the findings of our study suggested pegylated liposomal nanocarriers are proper for carboplatin delivery to ovarian cancer cell lines A2780S and A2780CP.</description><identifier>ISSN: 0970-1915</identifier><identifier>EISSN: 0974-0422</identifier><identifier>DOI: 10.1007/s12291-016-0596-3</identifier><identifier>PMID: 28428700</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Analysis ; Biochemistry ; Biomedical and Life Sciences ; Cancer ; Carboplatin ; Chemistry/Food Science ; Chemotherapy ; Cytotoxicity ; Health aspects ; Life Sciences ; Microbiology ; Morphology ; Nanoparticles ; Original ; Original Article ; Ovarian cancer ; Particle size ; Pathology</subject><ispartof>Indian journal of clinical biochemistry, 2017-06, Vol.32 (2), p.230-234</ispartof><rights>Association of Clinical Biochemists of India 2016</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Indian Journal of Clinical Biochemistry is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c674t-52fb5e06282fa9e3872cd3df0e835229f696b556e0ae8f0472bcd6973ddd90ae3</citedby><cites>FETCH-LOGICAL-c674t-52fb5e06282fa9e3872cd3df0e835229f696b556e0ae8f0472bcd6973ddd90ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382080/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5382080/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,41487,42556,51318,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28428700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ebrahimifar, Meysam</creatorcontrib><creatorcontrib>Nili-Ahmadabadi, Amir</creatorcontrib><creatorcontrib>Akbarzadeh, Azim</creatorcontrib><creatorcontrib>Shahemabadi, Hasan Ebrahimi</creatorcontrib><creatorcontrib>Hasanzadegan, Majid</creatorcontrib><creatorcontrib>Moradi-Sardareh, Hemen</creatorcontrib><creatorcontrib>Madadizadeh, Hassan</creatorcontrib><creatorcontrib>Rezaee-diyan, Jalal</creatorcontrib><title>Preparation, Characterization and Cytotoxic Effects of Pegylated Nanoliposomal Containing Carboplatin on Ovarian Cancer Cell Lines</title><title>Indian journal of clinical biochemistry</title><addtitle>Ind J Clin Biochem</addtitle><addtitle>Indian J Clin Biochem</addtitle><description>Carboplatin is a chemotherapeutic agent used against various malignancies such as ovarian carcinoma. The aim of this study is to improve the therapeutic efficacy of carboplatin using pegylated liposomal nanocarriers. Nanoparticles were synthesized using thin film hydration technique and characterized for shape morphology, particle size, zeta potential and drug-release properties. In the next step, A2780S and A2780CP ovarian cancer cell lines were used to determine the efficacy of nanodrug by MTT assay. The particle size and zeta potential of nanodrug were measured 244.3 ± 19.6 nm and −22.9 ± 1.7 mV, respectively. High encapsulation capacity (78.6 ± 3.7 %) confirmed the efficiency of technique. The cytotoxicity results also showed that nanodrug compared to free drug improve the efficacy of carboplatin against both A2780S (
P
< 0.01) and A2780CP (
P
< 0.05) cell lines. In conclusion, the findings of our study suggested pegylated liposomal nanocarriers are proper for carboplatin delivery to ovarian cancer cell lines A2780S and A2780CP.</description><subject>Analysis</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer</subject><subject>Carboplatin</subject><subject>Chemistry/Food Science</subject><subject>Chemotherapy</subject><subject>Cytotoxicity</subject><subject>Health aspects</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Morphology</subject><subject>Nanoparticles</subject><subject>Original</subject><subject>Original Article</subject><subject>Ovarian cancer</subject><subject>Particle size</subject><subject>Pathology</subject><issn>0970-1915</issn><issn>0974-0422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kl1rVDEQhg-i2Fr9Ad5IwBsFT53kfCU3QjlULSy2-HEdsjmT05SzyTbJlq6X_nKz3Vp2RclFhpln3jCTtyheUjimAN37SBkTtATaltCItqweFYcgurqEmrHHdzGUVNDmoHgW4xVAVUNNnxYHjNeMdwCHxa-LgEsVVLLevSP9ZQ51wmB_3mWIcgPp18knf2s1OTUGdYrEG3KB43pSCQfyRTk_2aWPfqEm0nuXlHXWjaRXYe6XGbKOZKnzGxWscjntNAbS4zSRmXUYnxdPjJoivri_j4ofH0-_95_L2fmns_5kVuq2q1PZMDNvEFrGmVECK94xPVSDAeRVk_dgWtHOm6ZFUMgN1B2b66EVXTUMg8i56qj4sNVdruYLHDS6FNQkl8EuVFhLr6zcrzh7KUd_I5uKM-CQBd7cCwR_vcKY5MJGnedQDv0qSsoF5RwE7zL6-i_0yq-Cy-NlitfQctHuUKOaUFpnfH5Xb0TlSUfr_FnA60wd_4PKZ8CF1d6hsTm_1_B2ryEzCW_TqFYxyrNvX_dZumV18DEGNA_7oCA3JpNbk8lsMrkxmaxyz6vdRT50_HFVBtgWiLnkRgw70_9X9TeMQtzD</recordid><startdate>20170601</startdate><enddate>20170601</enddate><creator>Ebrahimifar, Meysam</creator><creator>Nili-Ahmadabadi, Amir</creator><creator>Akbarzadeh, Azim</creator><creator>Shahemabadi, Hasan Ebrahimi</creator><creator>Hasanzadegan, Majid</creator><creator>Moradi-Sardareh, Hemen</creator><creator>Madadizadeh, Hassan</creator><creator>Rezaee-diyan, Jalal</creator><general>Springer India</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>04Q</scope><scope>04W</scope><scope>3V.</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20170601</creationdate><title>Preparation, Characterization and Cytotoxic Effects of Pegylated Nanoliposomal Containing Carboplatin on Ovarian Cancer Cell Lines</title><author>Ebrahimifar, Meysam ; Nili-Ahmadabadi, Amir ; Akbarzadeh, Azim ; Shahemabadi, Hasan Ebrahimi ; Hasanzadegan, Majid ; Moradi-Sardareh, Hemen ; Madadizadeh, Hassan ; Rezaee-diyan, Jalal</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c674t-52fb5e06282fa9e3872cd3df0e835229f696b556e0ae8f0472bcd6973ddd90ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analysis</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cancer</topic><topic>Carboplatin</topic><topic>Chemistry/Food Science</topic><topic>Chemotherapy</topic><topic>Cytotoxicity</topic><topic>Health aspects</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Morphology</topic><topic>Nanoparticles</topic><topic>Original</topic><topic>Original Article</topic><topic>Ovarian cancer</topic><topic>Particle size</topic><topic>Pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ebrahimifar, Meysam</creatorcontrib><creatorcontrib>Nili-Ahmadabadi, Amir</creatorcontrib><creatorcontrib>Akbarzadeh, Azim</creatorcontrib><creatorcontrib>Shahemabadi, Hasan Ebrahimi</creatorcontrib><creatorcontrib>Hasanzadegan, Majid</creatorcontrib><creatorcontrib>Moradi-Sardareh, Hemen</creatorcontrib><creatorcontrib>Madadizadeh, Hassan</creatorcontrib><creatorcontrib>Rezaee-diyan, Jalal</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>India Database</collection><collection>India Database: Science & Technology</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Indian journal of clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ebrahimifar, Meysam</au><au>Nili-Ahmadabadi, Amir</au><au>Akbarzadeh, Azim</au><au>Shahemabadi, Hasan Ebrahimi</au><au>Hasanzadegan, Majid</au><au>Moradi-Sardareh, Hemen</au><au>Madadizadeh, Hassan</au><au>Rezaee-diyan, Jalal</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation, Characterization and Cytotoxic Effects of Pegylated Nanoliposomal Containing Carboplatin on Ovarian Cancer Cell Lines</atitle><jtitle>Indian journal of clinical biochemistry</jtitle><stitle>Ind J Clin Biochem</stitle><addtitle>Indian J Clin Biochem</addtitle><date>2017-06-01</date><risdate>2017</risdate><volume>32</volume><issue>2</issue><spage>230</spage><epage>234</epage><pages>230-234</pages><issn>0970-1915</issn><eissn>0974-0422</eissn><abstract>Carboplatin is a chemotherapeutic agent used against various malignancies such as ovarian carcinoma. The aim of this study is to improve the therapeutic efficacy of carboplatin using pegylated liposomal nanocarriers. Nanoparticles were synthesized using thin film hydration technique and characterized for shape morphology, particle size, zeta potential and drug-release properties. In the next step, A2780S and A2780CP ovarian cancer cell lines were used to determine the efficacy of nanodrug by MTT assay. The particle size and zeta potential of nanodrug were measured 244.3 ± 19.6 nm and −22.9 ± 1.7 mV, respectively. High encapsulation capacity (78.6 ± 3.7 %) confirmed the efficiency of technique. The cytotoxicity results also showed that nanodrug compared to free drug improve the efficacy of carboplatin against both A2780S (
P
< 0.01) and A2780CP (
P
< 0.05) cell lines. In conclusion, the findings of our study suggested pegylated liposomal nanocarriers are proper for carboplatin delivery to ovarian cancer cell lines A2780S and A2780CP.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>28428700</pmid><doi>10.1007/s12291-016-0596-3</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Biochemistry Biomedical and Life Sciences Cancer Carboplatin Chemistry/Food Science Chemotherapy Cytotoxicity Health aspects Life Sciences Microbiology Morphology Nanoparticles Original Original Article Ovarian cancer Particle size Pathology |
| title | Preparation, Characterization and Cytotoxic Effects of Pegylated Nanoliposomal Containing Carboplatin on Ovarian Cancer Cell Lines |
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