Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device
The necessity for bone marrow aspiration and the lack of highly sensitive assays to detect residual disease present challenges for effective management of multiple myeloma (MM), a plasma cell cancer. We show that a microfluidic cell capture based on CD138 antigen, which is highly expressed on plasma...
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| Veröffentlicht in: | Scientific reports 2017-04, Vol.7 (1), p.45681-45681, Article 45681 |
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| creator | Qasaimeh, Mohammad A. Wu, Yichao C. Bose, Suman Menachery, Anoop Talluri, Srikanth Gonzalez, Gabriel Fulciniti, Mariateresa Karp, Jeffrey M. Prabhala, Rao H. Karnik, Rohit |
| description | The necessity for bone marrow aspiration and the lack of highly sensitive assays to detect residual disease present challenges for effective management of multiple myeloma (MM), a plasma cell cancer. We show that a microfluidic cell capture based on CD138 antigen, which is highly expressed on plasma cells, permits quantitation of rare circulating plasma cells (CPCs) in blood and subsequent fluorescence-based assays. The microfluidic device is based on a herringbone channel design, and exhibits an estimated cell capture efficiency of ~40–70%, permitting detection of |
| doi_str_mv | 10.1038/srep45681 |
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+
cells/mL in healthy donor blood, with significantly higher numbers in blood samples of MM patients in remission (20–24 CD138
+
cells/mL), and yet higher numbers in MM patients exhibiting disease (45–184 CD138
+
cells/mL). Analysis of CPCs isolated using the device was consistent with serum immunoglobulin assays that are commonly used in MM diagnostics. These results indicate the potential of CD138-based microfluidic CPC capture as a useful ‘liquid biopsy’ that may complement or partially replace bone marrow aspiration.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep45681</identifier><identifier>PMID: 28374831</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/51 ; 13/62 ; 631/1647/277 ; 631/67/1857 ; 639/166/985 ; 692/308/575 ; 9/10 ; Antibodies - metabolism ; Biopsy ; Bone marrow ; Bone Marrow - metabolism ; Bone Marrow - pathology ; Cancer ; CD138 antigen ; Cell Line, Tumor ; Flow cytometry ; Humanities and Social Sciences ; Humans ; Lab-On-A-Chip Devices ; Microfluidics ; multidisciplinary ; Multiple myeloma ; Multiple Myeloma - metabolism ; Multiple Myeloma - pathology ; Peripheral blood ; Plasma ; Plasma cells ; Plasma Cells - cytology ; Quantitation ; Remission ; Science ; Syndecan-1 - metabolism</subject><ispartof>Scientific reports, 2017-04, Vol.7 (1), p.45681-45681, Article 45681</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Apr 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-113c9e89ca2154a0e28b32ac9423e6fa863ed2783c3c93f867aec651b7a770c03</citedby><cites>FETCH-LOGICAL-c438t-113c9e89ca2154a0e28b32ac9423e6fa863ed2783c3c93f867aec651b7a770c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379479/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379479/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,42188,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28374831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qasaimeh, Mohammad A.</creatorcontrib><creatorcontrib>Wu, Yichao C.</creatorcontrib><creatorcontrib>Bose, Suman</creatorcontrib><creatorcontrib>Menachery, Anoop</creatorcontrib><creatorcontrib>Talluri, Srikanth</creatorcontrib><creatorcontrib>Gonzalez, Gabriel</creatorcontrib><creatorcontrib>Fulciniti, Mariateresa</creatorcontrib><creatorcontrib>Karp, Jeffrey M.</creatorcontrib><creatorcontrib>Prabhala, Rao H.</creatorcontrib><creatorcontrib>Karnik, Rohit</creatorcontrib><title>Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The necessity for bone marrow aspiration and the lack of highly sensitive assays to detect residual disease present challenges for effective management of multiple myeloma (MM), a plasma cell cancer. We show that a microfluidic cell capture based on CD138 antigen, which is highly expressed on plasma cells, permits quantitation of rare circulating plasma cells (CPCs) in blood and subsequent fluorescence-based assays. The microfluidic device is based on a herringbone channel design, and exhibits an estimated cell capture efficiency of ~40–70%, permitting detection of <10 CPCs/mL using 1-mL sample volumes, which is difficult using existing techniques. In bone marrow samples, the microfluidic-based plasma cell counts exhibited excellent correlation with flow cytometry analysis. In peripheral blood samples, the device detected a baseline of 2–5 CD138
+
cells/mL in healthy donor blood, with significantly higher numbers in blood samples of MM patients in remission (20–24 CD138
+
cells/mL), and yet higher numbers in MM patients exhibiting disease (45–184 CD138
+
cells/mL). Analysis of CPCs isolated using the device was consistent with serum immunoglobulin assays that are commonly used in MM diagnostics. These results indicate the potential of CD138-based microfluidic CPC capture as a useful ‘liquid biopsy’ that may complement or partially replace bone marrow aspiration.</description><subject>13/1</subject><subject>13/51</subject><subject>13/62</subject><subject>631/1647/277</subject><subject>631/67/1857</subject><subject>639/166/985</subject><subject>692/308/575</subject><subject>9/10</subject><subject>Antibodies - metabolism</subject><subject>Biopsy</subject><subject>Bone marrow</subject><subject>Bone Marrow - metabolism</subject><subject>Bone Marrow - pathology</subject><subject>Cancer</subject><subject>CD138 antigen</subject><subject>Cell Line, Tumor</subject><subject>Flow cytometry</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Lab-On-A-Chip Devices</subject><subject>Microfluidics</subject><subject>multidisciplinary</subject><subject>Multiple myeloma</subject><subject>Multiple Myeloma - metabolism</subject><subject>Multiple Myeloma - pathology</subject><subject>Peripheral blood</subject><subject>Plasma</subject><subject>Plasma cells</subject><subject>Plasma Cells - cytology</subject><subject>Quantitation</subject><subject>Remission</subject><subject>Science</subject><subject>Syndecan-1 - metabolism</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkUtv1DAUhS0EolXpgj-ALLEBpFC_EjsbpDalD6mjsqBry-PcDK48cbDjSvPv8Wja0QDe2Nb5fO71PQi9p-QrJVydpQiTqBtFX6FjRkRdMc7Y64PzETpN6ZGUVbNW0PYtOmKKS6E4PUb5NgVvZhdGHAbcuWjz9jqu8A9v0trgDrxP2I14kf3sJg94sQEfivKQtlh3SbnC5-PslqHfVBcmQY87M805wvaZwQtnYxh8dr2z-BKenIV36M1gfILT5_0EPVx9_9ndVHf317fd-V1lBVdzRSm3LajWGkZrYQgwteTM2FYwDs1gVMOhZ1JxWzg-qEYasE1Nl9JISSzhJ-jbznfKyzX0FsY5Gq-n6NYmbnQwTv-tjO6XXoUnXXPZCtkWg0_PBjH8zpBmvXbJlpGYEUJOmiolaKMaJQv68R_0MeQ4lu9p2hIuRFMzUajPO6rMJJXohn0zlOhtnnqfZ2E_HHa_J1_SK8CXHZCKNK4gHpT8z-0PjLKpmA</recordid><startdate>20170404</startdate><enddate>20170404</enddate><creator>Qasaimeh, Mohammad A.</creator><creator>Wu, Yichao C.</creator><creator>Bose, Suman</creator><creator>Menachery, Anoop</creator><creator>Talluri, Srikanth</creator><creator>Gonzalez, Gabriel</creator><creator>Fulciniti, Mariateresa</creator><creator>Karp, Jeffrey M.</creator><creator>Prabhala, Rao H.</creator><creator>Karnik, Rohit</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170404</creationdate><title>Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device</title><author>Qasaimeh, Mohammad A. ; Wu, Yichao C. ; Bose, Suman ; Menachery, Anoop ; Talluri, Srikanth ; Gonzalez, Gabriel ; Fulciniti, Mariateresa ; Karp, Jeffrey M. ; Prabhala, Rao H. ; Karnik, Rohit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-113c9e89ca2154a0e28b32ac9423e6fa863ed2783c3c93f867aec651b7a770c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/1</topic><topic>13/51</topic><topic>13/62</topic><topic>631/1647/277</topic><topic>631/67/1857</topic><topic>639/166/985</topic><topic>692/308/575</topic><topic>9/10</topic><topic>Antibodies - metabolism</topic><topic>Biopsy</topic><topic>Bone marrow</topic><topic>Bone Marrow - metabolism</topic><topic>Bone Marrow - pathology</topic><topic>Cancer</topic><topic>CD138 antigen</topic><topic>Cell Line, Tumor</topic><topic>Flow cytometry</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Lab-On-A-Chip Devices</topic><topic>Microfluidics</topic><topic>multidisciplinary</topic><topic>Multiple myeloma</topic><topic>Multiple Myeloma - metabolism</topic><topic>Multiple Myeloma - pathology</topic><topic>Peripheral blood</topic><topic>Plasma</topic><topic>Plasma cells</topic><topic>Plasma Cells - cytology</topic><topic>Quantitation</topic><topic>Remission</topic><topic>Science</topic><topic>Syndecan-1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qasaimeh, Mohammad A.</creatorcontrib><creatorcontrib>Wu, Yichao C.</creatorcontrib><creatorcontrib>Bose, Suman</creatorcontrib><creatorcontrib>Menachery, Anoop</creatorcontrib><creatorcontrib>Talluri, Srikanth</creatorcontrib><creatorcontrib>Gonzalez, Gabriel</creatorcontrib><creatorcontrib>Fulciniti, Mariateresa</creatorcontrib><creatorcontrib>Karp, Jeffrey M.</creatorcontrib><creatorcontrib>Prabhala, Rao H.</creatorcontrib><creatorcontrib>Karnik, Rohit</creatorcontrib><collection>SpringerOpen</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qasaimeh, Mohammad A.</au><au>Wu, Yichao C.</au><au>Bose, Suman</au><au>Menachery, Anoop</au><au>Talluri, Srikanth</au><au>Gonzalez, Gabriel</au><au>Fulciniti, Mariateresa</au><au>Karp, Jeffrey M.</au><au>Prabhala, Rao H.</au><au>Karnik, Rohit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-04-04</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>45681</spage><epage>45681</epage><pages>45681-45681</pages><artnum>45681</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The necessity for bone marrow aspiration and the lack of highly sensitive assays to detect residual disease present challenges for effective management of multiple myeloma (MM), a plasma cell cancer. We show that a microfluidic cell capture based on CD138 antigen, which is highly expressed on plasma cells, permits quantitation of rare circulating plasma cells (CPCs) in blood and subsequent fluorescence-based assays. The microfluidic device is based on a herringbone channel design, and exhibits an estimated cell capture efficiency of ~40–70%, permitting detection of <10 CPCs/mL using 1-mL sample volumes, which is difficult using existing techniques. In bone marrow samples, the microfluidic-based plasma cell counts exhibited excellent correlation with flow cytometry analysis. In peripheral blood samples, the device detected a baseline of 2–5 CD138
+
cells/mL in healthy donor blood, with significantly higher numbers in blood samples of MM patients in remission (20–24 CD138
+
cells/mL), and yet higher numbers in MM patients exhibiting disease (45–184 CD138
+
cells/mL). Analysis of CPCs isolated using the device was consistent with serum immunoglobulin assays that are commonly used in MM diagnostics. These results indicate the potential of CD138-based microfluidic CPC capture as a useful ‘liquid biopsy’ that may complement or partially replace bone marrow aspiration.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28374831</pmid><doi>10.1038/srep45681</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/1 13/51 13/62 631/1647/277 631/67/1857 639/166/985 692/308/575 9/10 Antibodies - metabolism Biopsy Bone marrow Bone Marrow - metabolism Bone Marrow - pathology Cancer CD138 antigen Cell Line, Tumor Flow cytometry Humanities and Social Sciences Humans Lab-On-A-Chip Devices Microfluidics multidisciplinary Multiple myeloma Multiple Myeloma - metabolism Multiple Myeloma - pathology Peripheral blood Plasma Plasma cells Plasma Cells - cytology Quantitation Remission Science Syndecan-1 - metabolism |
| title | Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device |
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