A submerged dielectric barrier discharge plasma inactivation mechanism of biofilms produced by Escherichia coli O157:H7, Cronobacter sakazakii, and Staphylococcus aureus
A submerged dielectric barrier discharge plasma reactor (underwater DBD) has been used to inactivate biofilm produced by three different food-borne pathogens, namely Escherichia coli O157:H7 (ATCC 438), Cronobacter sakazakii (ATCC 29004), and Staphylococcus aureus (KCCM 40050). The inactivation that...
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| Veröffentlicht in: | Scientific reports 2016-11, Vol.6 (1), p.37072, Article 37072 |
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| description | A submerged dielectric barrier discharge plasma reactor (underwater DBD) has been used to inactivate biofilm produced by three different food-borne pathogens, namely
Escherichia coli
O157:H7 (ATCC 438),
Cronobacter sakazakii
(ATCC 29004), and
Staphylococcus aureus
(KCCM 40050). The inactivation that were obtained after 90 minutes of plasma operation were found to measure 5.50 log CFU/coupon, 6.88 log CFU/coupon and 4.20 log CFU/coupon for
Escherichia coli
O157:H7 (ATCC 438),
Cronobacter sakazakii
(ATCC 29004), and
Staphylococcus aureus
(KCCM 40050), respectively. Secondary Electron Images (SEI) obtained from Field Emission Scanning Electron Microscopy (FE-SEM) show the biofilm morphology and its removal trend by plasma operation at different time intervals. An attenuated total reflectance Fourier transform infrared (ATR-FTIR) measurement was performed to elucidate the biochemical changes that occur on the bacterial cell and extracellular polymeric substance (EPS) of biofilm during the plasma inactivation process. The ATR-FTIR measurement shows the gradual reduction of carbohydrates, proteins, and lipid and DNA peak regions with increased plasma exposure time. The presence of an EPS layer on the upper surface of the biofilm plays a negative and significant role in its removal from stainless steel (SS) coupons. |
| doi_str_mv | 10.1038/srep37072 |
| format | Article |
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Escherichia coli
O157:H7 (ATCC 438),
Cronobacter sakazakii
(ATCC 29004), and
Staphylococcus aureus
(KCCM 40050). The inactivation that were obtained after 90 minutes of plasma operation were found to measure 5.50 log CFU/coupon, 6.88 log CFU/coupon and 4.20 log CFU/coupon for
Escherichia coli
O157:H7 (ATCC 438),
Cronobacter sakazakii
(ATCC 29004), and
Staphylococcus aureus
(KCCM 40050), respectively. Secondary Electron Images (SEI) obtained from Field Emission Scanning Electron Microscopy (FE-SEM) show the biofilm morphology and its removal trend by plasma operation at different time intervals. An attenuated total reflectance Fourier transform infrared (ATR-FTIR) measurement was performed to elucidate the biochemical changes that occur on the bacterial cell and extracellular polymeric substance (EPS) of biofilm during the plasma inactivation process. The ATR-FTIR measurement shows the gradual reduction of carbohydrates, proteins, and lipid and DNA peak regions with increased plasma exposure time. The presence of an EPS layer on the upper surface of the biofilm plays a negative and significant role in its removal from stainless steel (SS) coupons.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep37072</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/2196/2197 ; 631/326/2522 ; 631/326/421 ; 631/326/46 ; 631/61/252/22/1434 ; Biofilms ; Carbohydrates ; Colony-forming cells ; Deoxyribonucleic acid ; DNA ; E coli ; Electron microscopy ; Food ; Food contamination ; Fourier transforms ; Humanities and Social Sciences ; Inactivation ; multidisciplinary ; Pathogens ; Plasma ; Reflectance ; Scanning electron microscopy ; Science ; Stainless steel</subject><ispartof>Scientific reports, 2016-11, Vol.6 (1), p.37072, Article 37072</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Nov 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-25f66e64b63439395edafa6978cab3e3097327aaa4ccd16673e59ac69b0cf7d23</citedby><cites>FETCH-LOGICAL-c382t-25f66e64b63439395edafa6978cab3e3097327aaa4ccd16673e59ac69b0cf7d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378952/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378952/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,41125,42194,51581,53796,53798</link.rule.ids></links><search><creatorcontrib>Khan, Muhammad Saiful Islam</creatorcontrib><creatorcontrib>Lee, Eun-Jung</creatorcontrib><creatorcontrib>Kim, Yun-Ji</creatorcontrib><title>A submerged dielectric barrier discharge plasma inactivation mechanism of biofilms produced by Escherichia coli O157:H7, Cronobacter sakazakii, and Staphylococcus aureus</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><description>A submerged dielectric barrier discharge plasma reactor (underwater DBD) has been used to inactivate biofilm produced by three different food-borne pathogens, namely
Escherichia coli
O157:H7 (ATCC 438),
Cronobacter sakazakii
(ATCC 29004), and
Staphylococcus aureus
(KCCM 40050). The inactivation that were obtained after 90 minutes of plasma operation were found to measure 5.50 log CFU/coupon, 6.88 log CFU/coupon and 4.20 log CFU/coupon for
Escherichia coli
O157:H7 (ATCC 438),
Cronobacter sakazakii
(ATCC 29004), and
Staphylococcus aureus
(KCCM 40050), respectively. Secondary Electron Images (SEI) obtained from Field Emission Scanning Electron Microscopy (FE-SEM) show the biofilm morphology and its removal trend by plasma operation at different time intervals. An attenuated total reflectance Fourier transform infrared (ATR-FTIR) measurement was performed to elucidate the biochemical changes that occur on the bacterial cell and extracellular polymeric substance (EPS) of biofilm during the plasma inactivation process. The ATR-FTIR measurement shows the gradual reduction of carbohydrates, proteins, and lipid and DNA peak regions with increased plasma exposure time. The presence of an EPS layer on the upper surface of the biofilm plays a negative and significant role in its removal from stainless steel (SS) coupons.</description><subject>631/1647/2196/2197</subject><subject>631/326/2522</subject><subject>631/326/421</subject><subject>631/326/46</subject><subject>631/61/252/22/1434</subject><subject>Biofilms</subject><subject>Carbohydrates</subject><subject>Colony-forming cells</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E coli</subject><subject>Electron microscopy</subject><subject>Food</subject><subject>Food contamination</subject><subject>Fourier transforms</subject><subject>Humanities and Social Sciences</subject><subject>Inactivation</subject><subject>multidisciplinary</subject><subject>Pathogens</subject><subject>Plasma</subject><subject>Reflectance</subject><subject>Scanning electron microscopy</subject><subject>Science</subject><subject>Stainless steel</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkcFq3DAQhk1poCHNoW8g6Kkl29qSJVk9FMKSNoVADknOYjwe7yqxJVeyA5s36ltWy4aQUl0k5v_0jdAUxYeq_FKVovmaIk1Cl5q_KY55WcsVF5y_fXV-V5ymdF_mJbmpK3Nc_DlnaWlHihvqWOdoIJyjQ9ZCjI5iLiXcQk7ZNEAagTkPOLtHmF3wbKQcepdGFnrWutC7YUxsiqFbMPvaHbvI1ykLtw4YhsGx60rqb5f6jK1j8KHNstwlwQM8wYNzZwx8x25mmLa7IWBAXBKDJdKS3hdHPQyJTp_3k-Lux8Xt-nJ1df3z1_r8aoWi4fOKy14pUnWrRC2MMJI66EEZ3SC0gkRptOAaAGrErlJKC5IGUJm2xF53XJwU3w_eaf8vHZKfIwx2im6EuLMBnP038W5rN-HRSqEbI_eCj8-CGH4vlGZ7H5bo85tt1RgjhDLSZOrTgcIYUh5c_9KhKu1-mvZlmpn9fGBTZvyG4ivjf_BfS3alhA</recordid><startdate>20161115</startdate><enddate>20161115</enddate><creator>Khan, Muhammad Saiful Islam</creator><creator>Lee, Eun-Jung</creator><creator>Kim, Yun-Ji</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20161115</creationdate><title>A submerged dielectric barrier discharge plasma inactivation mechanism of biofilms produced by Escherichia coli O157:H7, Cronobacter sakazakii, and Staphylococcus aureus</title><author>Khan, Muhammad Saiful Islam ; Lee, Eun-Jung ; Kim, Yun-Ji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-25f66e64b63439395edafa6978cab3e3097327aaa4ccd16673e59ac69b0cf7d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>631/1647/2196/2197</topic><topic>631/326/2522</topic><topic>631/326/421</topic><topic>631/326/46</topic><topic>631/61/252/22/1434</topic><topic>Biofilms</topic><topic>Carbohydrates</topic><topic>Colony-forming cells</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>E coli</topic><topic>Electron microscopy</topic><topic>Food</topic><topic>Food contamination</topic><topic>Fourier transforms</topic><topic>Humanities and Social Sciences</topic><topic>Inactivation</topic><topic>multidisciplinary</topic><topic>Pathogens</topic><topic>Plasma</topic><topic>Reflectance</topic><topic>Scanning electron microscopy</topic><topic>Science</topic><topic>Stainless steel</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Muhammad Saiful Islam</creatorcontrib><creatorcontrib>Lee, Eun-Jung</creatorcontrib><creatorcontrib>Kim, Yun-Ji</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Muhammad Saiful Islam</au><au>Lee, Eun-Jung</au><au>Kim, Yun-Ji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A submerged dielectric barrier discharge plasma inactivation mechanism of biofilms produced by Escherichia coli O157:H7, Cronobacter sakazakii, and Staphylococcus aureus</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><date>2016-11-15</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>37072</spage><pages>37072-</pages><artnum>37072</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>A submerged dielectric barrier discharge plasma reactor (underwater DBD) has been used to inactivate biofilm produced by three different food-borne pathogens, namely
Escherichia coli
O157:H7 (ATCC 438),
Cronobacter sakazakii
(ATCC 29004), and
Staphylococcus aureus
(KCCM 40050). The inactivation that were obtained after 90 minutes of plasma operation were found to measure 5.50 log CFU/coupon, 6.88 log CFU/coupon and 4.20 log CFU/coupon for
Escherichia coli
O157:H7 (ATCC 438),
Cronobacter sakazakii
(ATCC 29004), and
Staphylococcus aureus
(KCCM 40050), respectively. Secondary Electron Images (SEI) obtained from Field Emission Scanning Electron Microscopy (FE-SEM) show the biofilm morphology and its removal trend by plasma operation at different time intervals. An attenuated total reflectance Fourier transform infrared (ATR-FTIR) measurement was performed to elucidate the biochemical changes that occur on the bacterial cell and extracellular polymeric substance (EPS) of biofilm during the plasma inactivation process. The ATR-FTIR measurement shows the gradual reduction of carbohydrates, proteins, and lipid and DNA peak regions with increased plasma exposure time. The presence of an EPS layer on the upper surface of the biofilm plays a negative and significant role in its removal from stainless steel (SS) coupons.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><doi>10.1038/srep37072</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 631/1647/2196/2197 631/326/2522 631/326/421 631/326/46 631/61/252/22/1434 Biofilms Carbohydrates Colony-forming cells Deoxyribonucleic acid DNA E coli Electron microscopy Food Food contamination Fourier transforms Humanities and Social Sciences Inactivation multidisciplinary Pathogens Plasma Reflectance Scanning electron microscopy Science Stainless steel |
| title | A submerged dielectric barrier discharge plasma inactivation mechanism of biofilms produced by Escherichia coli O157:H7, Cronobacter sakazakii, and Staphylococcus aureus |
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