Tumor‐derived spheroids: Relevance to cancer stem cells and clinical applications
Recently, many types of in vitro 3‐D culture systems have been developed to recapitulate the in vivo growth conditions of cancer. The cancer 3‐D culture methods aim to preserve the biological characteristics of original tumors better than conventional 2‐D monolayer cultures, and include tumor‐derive...
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| Veröffentlicht in: | Cancer science 2017-03, Vol.108 (3), p.283-289 |
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| creator | Ishiguro, Tatsuya Ohata, Hirokazu Sato, Ai Yamawaki, Kaoru Enomoto, Takayuki Okamoto, Koji |
| description | Recently, many types of in vitro 3‐D culture systems have been developed to recapitulate the in vivo growth conditions of cancer. The cancer 3‐D culture methods aim to preserve the biological characteristics of original tumors better than conventional 2‐D monolayer cultures, and include tumor‐derived organoids, tumor‐derived spheroids, organotypic multicellular spheroids, and multicellular tumor spheroids. The 3‐D culture methods differ in terms of cancer cell sources, protocols for cell handling, and the required time intervals. Tumor‐derived spheroids are unique because they are purposed for the enrichment of cancer stem cells (CSCs) or cells with stem cell‐related characteristics. These spheroids are grown as floating spheres and have been used as surrogate systems to evaluate the CSC‐related characteristics of solid tumors in vitro. Because eradication of CSCs is likely to be of clinical importance due to their association with the malignant nature of cancer cells, such as tumorigenicity or chemoresistance, the investigation of tumor‐derived spheroids may provide invaluable clues to fight against cancer. Spheroid cultures have been established from cancers including glioma, breast, colon, ovary, and prostate cancers, and their biological and biochemical characteristics have been investigated by many research groups. In addition to the investigation of CSCs, tumor‐derived spheroids may prove to be instrumental for a high‐throughput screening platform or for the cultivation of CSC‐related tumor cells found in the circulation or body fluids.
Tumor‐derived spheroid culture is one of the representative 3D culture methods in which cancer cells with stem cell‐like features are expanded in vitro as floating spheres. In this review, we summarize the major discoveries from studies using tumor‐derived spheroids and future clinical applications. |
| doi_str_mv | 10.1111/cas.13155 |
| format | Article |
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Tumor‐derived spheroid culture is one of the representative 3D culture methods in which cancer cells with stem cell‐like features are expanded in vitro as floating spheres. In this review, we summarize the major discoveries from studies using tumor‐derived spheroids and future clinical applications.</description><subject>Biochemical characteristics</subject><subject>Body fluids</subject><subject>Brain tumors</subject><subject>Breast</subject><subject>Cancer</subject><subject>Cancer stem cell</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Chemoresistance</subject><subject>Colon</subject><subject>Genetic engineering</subject><subject>Glioma</subject><subject>Growth conditions</subject><subject>Humans</subject><subject>Investigations</subject><subject>Kinases</subject><subject>Menopause</subject><subject>Methods</subject><subject>Multicellular tumor spheroids</subject><subject>Neoplasms - pathology</subject><subject>Neoplastic Stem Cells - cytology</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Organoids</subject><subject>Ovarian cancer</subject><subject>Prostate cancer</subject><subject>R&D</subject><subject>Research & development</subject><subject>Review</subject><subject>solid tumor</subject><subject>Solid tumors</subject><subject>Spheres</subject><subject>Spheroids</subject><subject>Spheroids, Cellular - cytology</subject><subject>Spheroids, Cellular - pathology</subject><subject>Stem cells</subject><subject>Therapeutic applications</subject><subject>three‐dimensional culture</subject><subject>Tumor cells</subject><subject>Tumor Cells, Cultured</subject><subject>Tumorigenicity</subject><subject>Tumors</subject><subject>tumor‐derived spheroid</subject><issn>1347-9032</issn><issn>1349-7006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc1qFTEYhoNYbK0uvAEJuNHFtPn_cVEoB38KBcHWdUiTjE3JTMZk5kh3XoLX6JWY09OWKghmkxfy8JDvewF4gdEBbufQ2XqAKeb8EdjDlOlOIiQe32TZaUTJLnha6xVCVDDNnoBdopBgjJE9cHa-DLn8-vHThxLXwcM6XYaSo69v4eeQwtqOLsA5Q7cJBdY5DNCFlCq0o4cuxTE6m6CdptTCHPNYn4Gd3qYant_e--DL-3fnq4_d6acPJ6vj085xIXjHibRSBeso10L2DvWWBiu8llJ4aRllXHqKg7c9RtyrC841RV45ySmhiNN9cLT1TsvFELwL41xsMlOJgy3XJtto_nwZ46X5mteGU6mIUE3w-lZQ8rcl1NkMsW6Gs2PISzVYaawkZpj9B8qFEoxy0dBXf6FXeSlj24QhRGmpGEG6UW-2lCu51hL6-39jZDatmtaquWm1sS8fDnpP3tXYgMMt8D2mcP1vk1kdn22VvwFi06x_</recordid><startdate>201703</startdate><enddate>201703</enddate><creator>Ishiguro, Tatsuya</creator><creator>Ohata, Hirokazu</creator><creator>Sato, Ai</creator><creator>Yamawaki, Kaoru</creator><creator>Enomoto, Takayuki</creator><creator>Okamoto, Koji</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TO</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>201703</creationdate><title>Tumor‐derived spheroids: Relevance to cancer stem cells and clinical applications</title><author>Ishiguro, Tatsuya ; 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The cancer 3‐D culture methods aim to preserve the biological characteristics of original tumors better than conventional 2‐D monolayer cultures, and include tumor‐derived organoids, tumor‐derived spheroids, organotypic multicellular spheroids, and multicellular tumor spheroids. The 3‐D culture methods differ in terms of cancer cell sources, protocols for cell handling, and the required time intervals. Tumor‐derived spheroids are unique because they are purposed for the enrichment of cancer stem cells (CSCs) or cells with stem cell‐related characteristics. These spheroids are grown as floating spheres and have been used as surrogate systems to evaluate the CSC‐related characteristics of solid tumors in vitro. Because eradication of CSCs is likely to be of clinical importance due to their association with the malignant nature of cancer cells, such as tumorigenicity or chemoresistance, the investigation of tumor‐derived spheroids may provide invaluable clues to fight against cancer. Spheroid cultures have been established from cancers including glioma, breast, colon, ovary, and prostate cancers, and their biological and biochemical characteristics have been investigated by many research groups. In addition to the investigation of CSCs, tumor‐derived spheroids may prove to be instrumental for a high‐throughput screening platform or for the cultivation of CSC‐related tumor cells found in the circulation or body fluids.
Tumor‐derived spheroid culture is one of the representative 3D culture methods in which cancer cells with stem cell‐like features are expanded in vitro as floating spheres. In this review, we summarize the major discoveries from studies using tumor‐derived spheroids and future clinical applications.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>28064442</pmid><doi>10.1111/cas.13155</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemical characteristics Body fluids Brain tumors Breast Cancer Cancer stem cell Cell adhesion & migration Cell culture Cell Culture Techniques Chemoresistance Colon Genetic engineering Glioma Growth conditions Humans Investigations Kinases Menopause Methods Multicellular tumor spheroids Neoplasms - pathology Neoplastic Stem Cells - cytology Neoplastic Stem Cells - pathology Organoids Ovarian cancer Prostate cancer R&D Research & development Review solid tumor Solid tumors Spheres Spheroids Spheroids, Cellular - cytology Spheroids, Cellular - pathology Stem cells Therapeutic applications three‐dimensional culture Tumor cells Tumor Cells, Cultured Tumorigenicity Tumors tumor‐derived spheroid |
| title | Tumor‐derived spheroids: Relevance to cancer stem cells and clinical applications |
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