Electroretinographic assessment of rod- and cone-mediated bipolar cell pathways using flicker stimuli in mice
Mouse full-field electroretinograms (ERGs) are dominated by responses of photoreceptors and depolarizing (ON-) bipolar cells, but not much of hyperpolarizing (OFF-) bipolar cells under conventional recording conditions. Here we investigate a novel ERG protocol in mice for functional assessment of th...
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| Veröffentlicht in: | Scientific reports 2015-06, Vol.5 (1), p.10731-10731, Article 10731 |
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| creator | Tanimoto, Naoyuki Sothilingam, Vithiyanjali Kondo, Mineo Biel, Martin Humphries, Peter Seeliger, Mathias W. |
| description | Mouse full-field electroretinograms (ERGs) are dominated by responses of photoreceptors and depolarizing (ON-) bipolar cells, but not much of hyperpolarizing (OFF-) bipolar cells under conventional recording conditions. Here we investigate a novel ERG protocol in mice for functional assessment of the major ON- and OFF-bipolar cell pathways using flicker stimuli for a high luminance with varying frequency up to 30 Hz. Wild-type (WT) and functionally specific transgenic mice (
Cnga3
-/-
, no cone photoreceptor function;
rho
-/-
, no rod photoreceptor function;
mGluR6
-/-
, no ON-bipolar cell function) were examined. The
Cnga3
-/-
flicker ERG was similar to the WT flicker ERG at very low stimulus frequencies, whereas ERGs were comparable between WT and
rho
-/-
mice at 5 Hz and above. Between 5 and 15 Hz, ERGs in
mGluR6
-/-
mice differed in configuration and amplitude from those in WT and
rho
-/-
mice; in contrast, response amplitudes above 15 Hz were comparable among WT,
rho
-/-
and
mGluR6
-/-
mice. In summary, we found three frequency ranges with these conditions that are dominated by activity in the rod pathways (below 5 Hz), cone ON-pathway (between 5 and 15 Hz) and cone OFF-pathway (above 15 Hz) that enables a quick overview of the functionality of the major bipolar cell pathways. |
| doi_str_mv | 10.1038/srep10731 |
| format | Article |
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Cnga3
-/-
, no cone photoreceptor function;
rho
-/-
, no rod photoreceptor function;
mGluR6
-/-
, no ON-bipolar cell function) were examined. The
Cnga3
-/-
flicker ERG was similar to the WT flicker ERG at very low stimulus frequencies, whereas ERGs were comparable between WT and
rho
-/-
mice at 5 Hz and above. Between 5 and 15 Hz, ERGs in
mGluR6
-/-
mice differed in configuration and amplitude from those in WT and
rho
-/-
mice; in contrast, response amplitudes above 15 Hz were comparable among WT,
rho
-/-
and
mGluR6
-/-
mice. In summary, we found three frequency ranges with these conditions that are dominated by activity in the rod pathways (below 5 Hz), cone ON-pathway (between 5 and 15 Hz) and cone OFF-pathway (above 15 Hz) that enables a quick overview of the functionality of the major bipolar cell pathways.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep10731</identifier><identifier>PMID: 26029863</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/1453 ; 631/443/376 ; 64/60 ; 9/30 ; Animals ; Bipolar cells ; Depolarization ; Electroretinograms ; Electroretinography ; Evoked Potentials, Visual ; Humanities and Social Sciences ; Mice ; Mice, Knockout ; multidisciplinary ; Photic Stimulation ; Photoreceptors ; Retinal Cone Photoreceptor Cells - metabolism ; Retinal Rod Photoreceptor Cells - metabolism ; Rodents ; Science ; Signal Transduction ; Transgenic mice</subject><ispartof>Scientific reports, 2015-06, Vol.5 (1), p.10731-10731, Article 10731</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Jun 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-be3f0cf71bb5439ca1b6e1b4117d3facccadfea71c30b7c62c6ac59f0f2475623</citedby><cites>FETCH-LOGICAL-c504t-be3f0cf71bb5439ca1b6e1b4117d3facccadfea71c30b7c62c6ac59f0f2475623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377071/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377071/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26029863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tanimoto, Naoyuki</creatorcontrib><creatorcontrib>Sothilingam, Vithiyanjali</creatorcontrib><creatorcontrib>Kondo, Mineo</creatorcontrib><creatorcontrib>Biel, Martin</creatorcontrib><creatorcontrib>Humphries, Peter</creatorcontrib><creatorcontrib>Seeliger, Mathias W.</creatorcontrib><title>Electroretinographic assessment of rod- and cone-mediated bipolar cell pathways using flicker stimuli in mice</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Mouse full-field electroretinograms (ERGs) are dominated by responses of photoreceptors and depolarizing (ON-) bipolar cells, but not much of hyperpolarizing (OFF-) bipolar cells under conventional recording conditions. Here we investigate a novel ERG protocol in mice for functional assessment of the major ON- and OFF-bipolar cell pathways using flicker stimuli for a high luminance with varying frequency up to 30 Hz. Wild-type (WT) and functionally specific transgenic mice (
Cnga3
-/-
, no cone photoreceptor function;
rho
-/-
, no rod photoreceptor function;
mGluR6
-/-
, no ON-bipolar cell function) were examined. The
Cnga3
-/-
flicker ERG was similar to the WT flicker ERG at very low stimulus frequencies, whereas ERGs were comparable between WT and
rho
-/-
mice at 5 Hz and above. Between 5 and 15 Hz, ERGs in
mGluR6
-/-
mice differed in configuration and amplitude from those in WT and
rho
-/-
mice; in contrast, response amplitudes above 15 Hz were comparable among WT,
rho
-/-
and
mGluR6
-/-
mice. In summary, we found three frequency ranges with these conditions that are dominated by activity in the rod pathways (below 5 Hz), cone ON-pathway (between 5 and 15 Hz) and cone OFF-pathway (above 15 Hz) that enables a quick overview of the functionality of the major bipolar cell pathways.</description><subject>631/1647/1453</subject><subject>631/443/376</subject><subject>64/60</subject><subject>9/30</subject><subject>Animals</subject><subject>Bipolar cells</subject><subject>Depolarization</subject><subject>Electroretinograms</subject><subject>Electroretinography</subject><subject>Evoked Potentials, Visual</subject><subject>Humanities and Social Sciences</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>multidisciplinary</subject><subject>Photic Stimulation</subject><subject>Photoreceptors</subject><subject>Retinal Cone Photoreceptor Cells - metabolism</subject><subject>Retinal Rod Photoreceptor Cells - metabolism</subject><subject>Rodents</subject><subject>Science</subject><subject>Signal Transduction</subject><subject>Transgenic mice</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNplkUtr3DAUhUVpaUKSRf9AEXTTFpzoYVn2plBC-oBAN-layNdXM0ptyZXslPz7aph0mLbaXMH9OPccDiGvOLvkTLZXOeHMmZb8GTkVrFaVkEI8P_qfkIuc71l5SnQ1716SE9Ew0bWNPCXTzYiwpJhw8SFukp23HqjNGXOeMCw0OpriUFEbBgoxYDXh4O2CA-39HEebKOA40tku21_2MdM1-7ChbvTwAxPNi5_W0VMf6OQBz8kLZ8eMF0_zjHz_dHN3_aW6_fb56_XH2woUq5eqR-kYOM37XtWyA8v7Bnlfc64H6SwA2MGh1Rwk6zU0AhoLqnPMiVqrRsgz8mGvO6998QslSLKjmZOfbHo00Xrz9yb4rdnEB6Ok1kzzIvD2SSDFnyvmxUw-74LagHHNhjet0kppubv15h_0Pq4plHiGt12nVMskK9S7PQUp5lKZO5jhzOx6NIceC_v62P2B_NNaAd7vgVxWYYPp6OR_ar8B5ZmqHA</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Tanimoto, Naoyuki</creator><creator>Sothilingam, Vithiyanjali</creator><creator>Kondo, Mineo</creator><creator>Biel, Martin</creator><creator>Humphries, Peter</creator><creator>Seeliger, Mathias W.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150601</creationdate><title>Electroretinographic assessment of rod- and cone-mediated bipolar cell pathways using flicker stimuli in mice</title><author>Tanimoto, Naoyuki ; Sothilingam, Vithiyanjali ; Kondo, Mineo ; Biel, Martin ; Humphries, Peter ; Seeliger, Mathias W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-be3f0cf71bb5439ca1b6e1b4117d3facccadfea71c30b7c62c6ac59f0f2475623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>631/1647/1453</topic><topic>631/443/376</topic><topic>64/60</topic><topic>9/30</topic><topic>Animals</topic><topic>Bipolar cells</topic><topic>Depolarization</topic><topic>Electroretinograms</topic><topic>Electroretinography</topic><topic>Evoked Potentials, Visual</topic><topic>Humanities and Social Sciences</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>multidisciplinary</topic><topic>Photic Stimulation</topic><topic>Photoreceptors</topic><topic>Retinal Cone Photoreceptor Cells - metabolism</topic><topic>Retinal Rod Photoreceptor Cells - metabolism</topic><topic>Rodents</topic><topic>Science</topic><topic>Signal Transduction</topic><topic>Transgenic mice</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tanimoto, Naoyuki</creatorcontrib><creatorcontrib>Sothilingam, Vithiyanjali</creatorcontrib><creatorcontrib>Kondo, Mineo</creatorcontrib><creatorcontrib>Biel, Martin</creatorcontrib><creatorcontrib>Humphries, Peter</creatorcontrib><creatorcontrib>Seeliger, Mathias W.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanimoto, Naoyuki</au><au>Sothilingam, Vithiyanjali</au><au>Kondo, Mineo</au><au>Biel, Martin</au><au>Humphries, Peter</au><au>Seeliger, Mathias W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electroretinographic assessment of rod- and cone-mediated bipolar cell pathways using flicker stimuli in mice</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>10731</spage><epage>10731</epage><pages>10731-10731</pages><artnum>10731</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Mouse full-field electroretinograms (ERGs) are dominated by responses of photoreceptors and depolarizing (ON-) bipolar cells, but not much of hyperpolarizing (OFF-) bipolar cells under conventional recording conditions. Here we investigate a novel ERG protocol in mice for functional assessment of the major ON- and OFF-bipolar cell pathways using flicker stimuli for a high luminance with varying frequency up to 30 Hz. Wild-type (WT) and functionally specific transgenic mice (
Cnga3
-/-
, no cone photoreceptor function;
rho
-/-
, no rod photoreceptor function;
mGluR6
-/-
, no ON-bipolar cell function) were examined. The
Cnga3
-/-
flicker ERG was similar to the WT flicker ERG at very low stimulus frequencies, whereas ERGs were comparable between WT and
rho
-/-
mice at 5 Hz and above. Between 5 and 15 Hz, ERGs in
mGluR6
-/-
mice differed in configuration and amplitude from those in WT and
rho
-/-
mice; in contrast, response amplitudes above 15 Hz were comparable among WT,
rho
-/-
and
mGluR6
-/-
mice. In summary, we found three frequency ranges with these conditions that are dominated by activity in the rod pathways (below 5 Hz), cone ON-pathway (between 5 and 15 Hz) and cone OFF-pathway (above 15 Hz) that enables a quick overview of the functionality of the major bipolar cell pathways.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26029863</pmid><doi>10.1038/srep10731</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 631/1647/1453 631/443/376 64/60 9/30 Animals Bipolar cells Depolarization Electroretinograms Electroretinography Evoked Potentials, Visual Humanities and Social Sciences Mice Mice, Knockout multidisciplinary Photic Stimulation Photoreceptors Retinal Cone Photoreceptor Cells - metabolism Retinal Rod Photoreceptor Cells - metabolism Rodents Science Signal Transduction Transgenic mice |
| title | Electroretinographic assessment of rod- and cone-mediated bipolar cell pathways using flicker stimuli in mice |
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