Aqueous humour concentrations of TGF‐β, PLGF and FGF‐1 and total retinal blood flow in patients with early non‐proliferative diabetic retinopathy
Purpose To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non‐proliferative diabetic retinopathy (NPDR). Methods A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the st...
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| Veröffentlicht in: | Acta ophthalmologica (Oxford, England) England), 2017-05, Vol.95 (3), p.e206-e211 |
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| creator | Khuu, Lee‐Anne Tayyari, Faryan Sivak, Jeremy M. Flanagan, John G. Singer, Shaun Brent, Michael H. Huang, David Tan, Ou Hudson, Christopher |
| description | Purpose
To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non‐proliferative diabetic retinopathy (NPDR).
Methods
A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue™ Doppler Fourier domain optical coherence tomography (Doppler FD‐OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c).
Results
Transforming growth factor beta (TGF‐β1, TGF‐β2) and PLGF were increased while FGF‐1 was reduced in NPDR compared to controls (Bonferroni corrected, p |
| doi_str_mv | 10.1111/aos.13230 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5373930</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1841127044</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3630-4c7971365599d271585ff2209c66f20c4a024e2eda53ff1aeaff71e73193e53a3</originalsourceid><addsrcrecordid>eNqNkc9uEzEQxq0K1JbCoS-AfKQSaf1nbe9ekKKKBKRIRaJI3CzHazeuHDvYu41y4xE48hw8CA_Bk-BkS0QPlerLjOxvfuOZD4BTjM5xORcq5nNMCUUH4BgLxkZU8PrZPmdfj8CLnG8R4pjz6hAcEcFFTRA-Bj_H33oT-wwX_TL2CeoYtAldUp2LIcNo4fV08uf7j9-_3sJPs-kEqtDCye4K7_IudsrDZDoXSpz7GFtofVxDF-CqUAosw7XrFtCo5DcwxFBqVyl6Z822zZ2BrVPzAtADJpayxeYleG6Vz-bVfTwBXybvry8_jGZX04-X49lIU07RqNKiEZhyxpqmJQKzmllLCGo055YgXSlEKkNMqxi1FiujrBXYCIobahhV9AS8G7irfr407TC8l6vkliptZFROPnwJbiFv4p1kVNCGogJ4cw9Isewyd3Lpsjbeq7BdrMR1g-uaC149QVphTASqttKzQapTzDkZu_8RRnJruiymy53pRfv6_xH2yn8uF8HFIFg7bzaPk-T46vOA_AsMcLw9</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1841127044</pqid></control><display><type>article</type><title>Aqueous humour concentrations of TGF‐β, PLGF and FGF‐1 and total retinal blood flow in patients with early non‐proliferative diabetic retinopathy</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>Wiley Online Library (Open Access Collection)</source><creator>Khuu, Lee‐Anne ; Tayyari, Faryan ; Sivak, Jeremy M. ; Flanagan, John G. ; Singer, Shaun ; Brent, Michael H. ; Huang, David ; Tan, Ou ; Hudson, Christopher</creator><creatorcontrib>Khuu, Lee‐Anne ; Tayyari, Faryan ; Sivak, Jeremy M. ; Flanagan, John G. ; Singer, Shaun ; Brent, Michael H. ; Huang, David ; Tan, Ou ; Hudson, Christopher</creatorcontrib><description>Purpose
To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non‐proliferative diabetic retinopathy (NPDR).
Methods
A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue™ Doppler Fourier domain optical coherence tomography (Doppler FD‐OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c).
Results
Transforming growth factor beta (TGF‐β1, TGF‐β2) and PLGF were increased while FGF‐1 was reduced in NPDR compared to controls (Bonferroni corrected, p < 0.003 for all). Total retinal blood flow (TRBF) was significantly reduced in the NPDR group compared to controls (33.1 ± 9.9 versus 43.3 ± 5.3 μl/min, p = 0.002). Aqueous FGF‐1 significantly correlated with TRBF in the NPDR group (r = 0.71, p = 0.01; r2 = 0.51). In a multiple regression analysis, A1c was found to be a significant predictor of aqueous TGF‐β1 and FGF‐1 (p = 0.018 and p = 0.020, respectively).
Conclusion
Aqueous angiogenic cytokines (TGF‐β1, TGF‐β2 and PLGF) were elevated in conjunction with a reduction in TRBF in patients with NPDR compared to controls. Non‐invasive measurement of TRBF may be useful for predicting aqueous FGF‐1 levels and severity of vasculopathy in DR.</description><identifier>ISSN: 1755-375X</identifier><identifier>EISSN: 1755-3768</identifier><identifier>DOI: 10.1111/aos.13230</identifier><identifier>PMID: 27678201</identifier><language>eng</language><publisher>England</publisher><subject>Aged ; angiogenesis ; Aqueous Humor - metabolism ; aqueous humour cytokines ; Biomarkers - metabolism ; Diabetes Mellitus, Type 2 - metabolism ; Diabetes Mellitus, Type 2 - physiopathology ; Diabetic Retinopathy - diagnosis ; Diabetic Retinopathy - metabolism ; Diabetic Retinopathy - physiopathology ; Female ; fibroblast growth factor ; Fibroblast Growth Factor 1 - metabolism ; Humans ; Male ; non‐proliferative diabetic retinopathy ; optical coherence tomography ; Placenta Growth Factor - metabolism ; Regional Blood Flow - physiology ; retinal blood flow ; Retinal Vessels - diagnostic imaging ; Retinal Vessels - physiopathology ; Tomography, Optical Coherence - methods ; Transforming Growth Factor beta - metabolism ; type 2 diabetes mellitus ; vasoregulatory</subject><ispartof>Acta ophthalmologica (Oxford, England), 2017-05, Vol.95 (3), p.e206-e211</ispartof><rights>2016 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd</rights><rights>2016 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3630-4c7971365599d271585ff2209c66f20c4a024e2eda53ff1aeaff71e73193e53a3</citedby><cites>FETCH-LOGICAL-c3630-4c7971365599d271585ff2209c66f20c4a024e2eda53ff1aeaff71e73193e53a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Faos.13230$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Faos.13230$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,782,786,887,1419,1435,27931,27932,45581,45582,46416,46840</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27678201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khuu, Lee‐Anne</creatorcontrib><creatorcontrib>Tayyari, Faryan</creatorcontrib><creatorcontrib>Sivak, Jeremy M.</creatorcontrib><creatorcontrib>Flanagan, John G.</creatorcontrib><creatorcontrib>Singer, Shaun</creatorcontrib><creatorcontrib>Brent, Michael H.</creatorcontrib><creatorcontrib>Huang, David</creatorcontrib><creatorcontrib>Tan, Ou</creatorcontrib><creatorcontrib>Hudson, Christopher</creatorcontrib><title>Aqueous humour concentrations of TGF‐β, PLGF and FGF‐1 and total retinal blood flow in patients with early non‐proliferative diabetic retinopathy</title><title>Acta ophthalmologica (Oxford, England)</title><addtitle>Acta Ophthalmol</addtitle><description>Purpose
To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non‐proliferative diabetic retinopathy (NPDR).
Methods
A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue™ Doppler Fourier domain optical coherence tomography (Doppler FD‐OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c).
Results
Transforming growth factor beta (TGF‐β1, TGF‐β2) and PLGF were increased while FGF‐1 was reduced in NPDR compared to controls (Bonferroni corrected, p < 0.003 for all). Total retinal blood flow (TRBF) was significantly reduced in the NPDR group compared to controls (33.1 ± 9.9 versus 43.3 ± 5.3 μl/min, p = 0.002). Aqueous FGF‐1 significantly correlated with TRBF in the NPDR group (r = 0.71, p = 0.01; r2 = 0.51). In a multiple regression analysis, A1c was found to be a significant predictor of aqueous TGF‐β1 and FGF‐1 (p = 0.018 and p = 0.020, respectively).
Conclusion
Aqueous angiogenic cytokines (TGF‐β1, TGF‐β2 and PLGF) were elevated in conjunction with a reduction in TRBF in patients with NPDR compared to controls. Non‐invasive measurement of TRBF may be useful for predicting aqueous FGF‐1 levels and severity of vasculopathy in DR.</description><subject>Aged</subject><subject>angiogenesis</subject><subject>Aqueous Humor - metabolism</subject><subject>aqueous humour cytokines</subject><subject>Biomarkers - metabolism</subject><subject>Diabetes Mellitus, Type 2 - metabolism</subject><subject>Diabetes Mellitus, Type 2 - physiopathology</subject><subject>Diabetic Retinopathy - diagnosis</subject><subject>Diabetic Retinopathy - metabolism</subject><subject>Diabetic Retinopathy - physiopathology</subject><subject>Female</subject><subject>fibroblast growth factor</subject><subject>Fibroblast Growth Factor 1 - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>non‐proliferative diabetic retinopathy</subject><subject>optical coherence tomography</subject><subject>Placenta Growth Factor - metabolism</subject><subject>Regional Blood Flow - physiology</subject><subject>retinal blood flow</subject><subject>Retinal Vessels - diagnostic imaging</subject><subject>Retinal Vessels - physiopathology</subject><subject>Tomography, Optical Coherence - methods</subject><subject>Transforming Growth Factor beta - metabolism</subject><subject>type 2 diabetes mellitus</subject><subject>vasoregulatory</subject><issn>1755-375X</issn><issn>1755-3768</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9uEzEQxq0K1JbCoS-AfKQSaf1nbe9ekKKKBKRIRaJI3CzHazeuHDvYu41y4xE48hw8CA_Bk-BkS0QPlerLjOxvfuOZD4BTjM5xORcq5nNMCUUH4BgLxkZU8PrZPmdfj8CLnG8R4pjz6hAcEcFFTRA-Bj_H33oT-wwX_TL2CeoYtAldUp2LIcNo4fV08uf7j9-_3sJPs-kEqtDCye4K7_IudsrDZDoXSpz7GFtofVxDF-CqUAosw7XrFtCo5DcwxFBqVyl6Z822zZ2BrVPzAtADJpayxeYleG6Vz-bVfTwBXybvry8_jGZX04-X49lIU07RqNKiEZhyxpqmJQKzmllLCGo055YgXSlEKkNMqxi1FiujrBXYCIobahhV9AS8G7irfr407TC8l6vkliptZFROPnwJbiFv4p1kVNCGogJ4cw9Isewyd3Lpsjbeq7BdrMR1g-uaC149QVphTASqttKzQapTzDkZu_8RRnJruiymy53pRfv6_xH2yn8uF8HFIFg7bzaPk-T46vOA_AsMcLw9</recordid><startdate>201705</startdate><enddate>201705</enddate><creator>Khuu, Lee‐Anne</creator><creator>Tayyari, Faryan</creator><creator>Sivak, Jeremy M.</creator><creator>Flanagan, John G.</creator><creator>Singer, Shaun</creator><creator>Brent, Michael H.</creator><creator>Huang, David</creator><creator>Tan, Ou</creator><creator>Hudson, Christopher</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>201705</creationdate><title>Aqueous humour concentrations of TGF‐β, PLGF and FGF‐1 and total retinal blood flow in patients with early non‐proliferative diabetic retinopathy</title><author>Khuu, Lee‐Anne ; Tayyari, Faryan ; Sivak, Jeremy M. ; Flanagan, John G. ; Singer, Shaun ; Brent, Michael H. ; Huang, David ; Tan, Ou ; Hudson, Christopher</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3630-4c7971365599d271585ff2209c66f20c4a024e2eda53ff1aeaff71e73193e53a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aged</topic><topic>angiogenesis</topic><topic>Aqueous Humor - metabolism</topic><topic>aqueous humour cytokines</topic><topic>Biomarkers - metabolism</topic><topic>Diabetes Mellitus, Type 2 - metabolism</topic><topic>Diabetes Mellitus, Type 2 - physiopathology</topic><topic>Diabetic Retinopathy - diagnosis</topic><topic>Diabetic Retinopathy - metabolism</topic><topic>Diabetic Retinopathy - physiopathology</topic><topic>Female</topic><topic>fibroblast growth factor</topic><topic>Fibroblast Growth Factor 1 - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>non‐proliferative diabetic retinopathy</topic><topic>optical coherence tomography</topic><topic>Placenta Growth Factor - metabolism</topic><topic>Regional Blood Flow - physiology</topic><topic>retinal blood flow</topic><topic>Retinal Vessels - diagnostic imaging</topic><topic>Retinal Vessels - physiopathology</topic><topic>Tomography, Optical Coherence - methods</topic><topic>Transforming Growth Factor beta - metabolism</topic><topic>type 2 diabetes mellitus</topic><topic>vasoregulatory</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khuu, Lee‐Anne</creatorcontrib><creatorcontrib>Tayyari, Faryan</creatorcontrib><creatorcontrib>Sivak, Jeremy M.</creatorcontrib><creatorcontrib>Flanagan, John G.</creatorcontrib><creatorcontrib>Singer, Shaun</creatorcontrib><creatorcontrib>Brent, Michael H.</creatorcontrib><creatorcontrib>Huang, David</creatorcontrib><creatorcontrib>Tan, Ou</creatorcontrib><creatorcontrib>Hudson, Christopher</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta ophthalmologica (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khuu, Lee‐Anne</au><au>Tayyari, Faryan</au><au>Sivak, Jeremy M.</au><au>Flanagan, John G.</au><au>Singer, Shaun</au><au>Brent, Michael H.</au><au>Huang, David</au><au>Tan, Ou</au><au>Hudson, Christopher</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aqueous humour concentrations of TGF‐β, PLGF and FGF‐1 and total retinal blood flow in patients with early non‐proliferative diabetic retinopathy</atitle><jtitle>Acta ophthalmologica (Oxford, England)</jtitle><addtitle>Acta Ophthalmol</addtitle><date>2017-05</date><risdate>2017</risdate><volume>95</volume><issue>3</issue><spage>e206</spage><epage>e211</epage><pages>e206-e211</pages><issn>1755-375X</issn><eissn>1755-3768</eissn><abstract>Purpose
To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non‐proliferative diabetic retinopathy (NPDR).
Methods
A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue™ Doppler Fourier domain optical coherence tomography (Doppler FD‐OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c).
Results
Transforming growth factor beta (TGF‐β1, TGF‐β2) and PLGF were increased while FGF‐1 was reduced in NPDR compared to controls (Bonferroni corrected, p < 0.003 for all). Total retinal blood flow (TRBF) was significantly reduced in the NPDR group compared to controls (33.1 ± 9.9 versus 43.3 ± 5.3 μl/min, p = 0.002). Aqueous FGF‐1 significantly correlated with TRBF in the NPDR group (r = 0.71, p = 0.01; r2 = 0.51). In a multiple regression analysis, A1c was found to be a significant predictor of aqueous TGF‐β1 and FGF‐1 (p = 0.018 and p = 0.020, respectively).
Conclusion
Aqueous angiogenic cytokines (TGF‐β1, TGF‐β2 and PLGF) were elevated in conjunction with a reduction in TRBF in patients with NPDR compared to controls. Non‐invasive measurement of TRBF may be useful for predicting aqueous FGF‐1 levels and severity of vasculopathy in DR.</abstract><cop>England</cop><pmid>27678201</pmid><doi>10.1111/aos.13230</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged angiogenesis Aqueous Humor - metabolism aqueous humour cytokines Biomarkers - metabolism Diabetes Mellitus, Type 2 - metabolism Diabetes Mellitus, Type 2 - physiopathology Diabetic Retinopathy - diagnosis Diabetic Retinopathy - metabolism Diabetic Retinopathy - physiopathology Female fibroblast growth factor Fibroblast Growth Factor 1 - metabolism Humans Male non‐proliferative diabetic retinopathy optical coherence tomography Placenta Growth Factor - metabolism Regional Blood Flow - physiology retinal blood flow Retinal Vessels - diagnostic imaging Retinal Vessels - physiopathology Tomography, Optical Coherence - methods Transforming Growth Factor beta - metabolism type 2 diabetes mellitus vasoregulatory |
| title | Aqueous humour concentrations of TGF‐β, PLGF and FGF‐1 and total retinal blood flow in patients with early non‐proliferative diabetic retinopathy |
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