Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs
Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells...
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| Veröffentlicht in: | Scientific reports 2017-03, Vol.7 (1), p.45607-45607, Article 45607 |
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| creator | Su, Long-Jyun Wu, Meng-Shiue Hui, Yuen Yung Chang, Be-Ming Pan, Lei Hsu, Pei-Chen Chen, Yit-Tsong Ho, Hong-Nerng Huang, Yen-Hua Ling, Thai-Yen Hsu, Hsao-Hsun Chang, Huan-Cheng |
| description | Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well. |
| doi_str_mv | 10.1038/srep45607 |
| format | Article |
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While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep45607</identifier><identifier>PMID: 28358111</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/31 ; 13/51 ; 14/19 ; 14/34 ; 140/125 ; 631/1647/245/2225 ; 631/61/2320 ; 639/925/350/354 ; 639/925/352/2734 ; 64 ; A549 Cells ; Albumin ; Animal models ; Animals ; Biocompatible Materials ; Cell Tracking - methods ; HeLa Cells ; Humanities and Social Sciences ; Humans ; Intravenous administration ; Lung - cytology ; Mesenchymal Stem Cell Transplantation - methods ; Mesenchymal stem cells ; Mesenchymal Stem Cells - physiology ; Mesenchyme ; Microscopy, Fluorescence - methods ; multidisciplinary ; Nanodiamonds - administration & dosage ; Nanodiamonds - chemistry ; Pharmacokinetics ; Placenta ; Rodents ; Science ; Serum Albumin, Human - administration & dosage ; Serum Albumin, Human - chemistry ; Signal-To-Noise Ratio ; Stem cell transplantation ; Stem cells ; Swine ; Swine, Miniature ; Therapeutic applications ; Tissue Distribution</subject><ispartof>Scientific reports, 2017-03, Vol.7 (1), p.45607-45607, Article 45607</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Mar 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-7cc66f42cc5a24fe069f725ab604474b3f1d43f16e60e6e3d7ae1f828d3752573</citedby><cites>FETCH-LOGICAL-c504t-7cc66f42cc5a24fe069f725ab604474b3f1d43f16e60e6e3d7ae1f828d3752573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372358/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372358/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28358111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Long-Jyun</creatorcontrib><creatorcontrib>Wu, Meng-Shiue</creatorcontrib><creatorcontrib>Hui, Yuen Yung</creatorcontrib><creatorcontrib>Chang, Be-Ming</creatorcontrib><creatorcontrib>Pan, Lei</creatorcontrib><creatorcontrib>Hsu, Pei-Chen</creatorcontrib><creatorcontrib>Chen, Yit-Tsong</creatorcontrib><creatorcontrib>Ho, Hong-Nerng</creatorcontrib><creatorcontrib>Huang, Yen-Hua</creatorcontrib><creatorcontrib>Ling, Thai-Yen</creatorcontrib><creatorcontrib>Hsu, Hsao-Hsun</creatorcontrib><creatorcontrib>Chang, Huan-Cheng</creatorcontrib><title>Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well.</description><subject>13/31</subject><subject>13/51</subject><subject>14/19</subject><subject>14/34</subject><subject>140/125</subject><subject>631/1647/245/2225</subject><subject>631/61/2320</subject><subject>639/925/350/354</subject><subject>639/925/352/2734</subject><subject>64</subject><subject>A549 Cells</subject><subject>Albumin</subject><subject>Animal models</subject><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Cell Tracking - methods</subject><subject>HeLa Cells</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Intravenous administration</subject><subject>Lung - cytology</subject><subject>Mesenchymal Stem Cell Transplantation - methods</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - physiology</subject><subject>Mesenchyme</subject><subject>Microscopy, Fluorescence - methods</subject><subject>multidisciplinary</subject><subject>Nanodiamonds - administration & dosage</subject><subject>Nanodiamonds - chemistry</subject><subject>Pharmacokinetics</subject><subject>Placenta</subject><subject>Rodents</subject><subject>Science</subject><subject>Serum Albumin, Human - administration & dosage</subject><subject>Serum Albumin, Human - chemistry</subject><subject>Signal-To-Noise Ratio</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Swine</subject><subject>Swine, Miniature</subject><subject>Therapeutic applications</subject><subject>Tissue Distribution</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNplkV1LHDEUhkOpVFm96B-QgDetsDbfmb0RitQPELyx1yGbOTMbnUnWJCP475tl7bK2uUgC5-E95z0vQl8puaCENz9ygrWQiuhP6IgRIeeMM_Z573-ITnJ-IvVIthB08QUdsobLhlJ6hPrrYYoJsoNQcLAhtt6OMbQZQ7DLAfDLZEPxxRb_Crgk65596HHs8GoabcAjZAhu9TbaAecCI3YwDBn7WvHB2zIlwGvf52N00Nkhw8n7O0O_r389Xt3O7x9u7q5-3s-dJKLMtXNKdYI5Jy0THRC16DSTdqmIEFoseUdbUS8FioAC3moLtGtY03ItmdR8hi63uutpOUK7sZXsYNbJjza9mWi9-VgJfmX6-Gok16wupQp8exdI8WWCXMzo88aUDRCnbGjTMFlXyVlFz_5Bn-KUQrVn6IJw3ihduRn6vqVcirmG1e2GocRsEjS7BCt7uj_9jvybVwXOt0CupdBD2mv5n9ofuYOnGQ</recordid><startdate>20170330</startdate><enddate>20170330</enddate><creator>Su, Long-Jyun</creator><creator>Wu, Meng-Shiue</creator><creator>Hui, Yuen Yung</creator><creator>Chang, Be-Ming</creator><creator>Pan, Lei</creator><creator>Hsu, Pei-Chen</creator><creator>Chen, Yit-Tsong</creator><creator>Ho, Hong-Nerng</creator><creator>Huang, Yen-Hua</creator><creator>Ling, Thai-Yen</creator><creator>Hsu, Hsao-Hsun</creator><creator>Chang, Huan-Cheng</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170330</creationdate><title>Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs</title><author>Su, Long-Jyun ; Wu, Meng-Shiue ; Hui, Yuen Yung ; Chang, Be-Ming ; Pan, Lei ; Hsu, Pei-Chen ; Chen, Yit-Tsong ; Ho, Hong-Nerng ; Huang, Yen-Hua ; Ling, Thai-Yen ; Hsu, Hsao-Hsun ; Chang, Huan-Cheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-7cc66f42cc5a24fe069f725ab604474b3f1d43f16e60e6e3d7ae1f828d3752573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/31</topic><topic>13/51</topic><topic>14/19</topic><topic>14/34</topic><topic>140/125</topic><topic>631/1647/245/2225</topic><topic>631/61/2320</topic><topic>639/925/350/354</topic><topic>639/925/352/2734</topic><topic>64</topic><topic>A549 Cells</topic><topic>Albumin</topic><topic>Animal models</topic><topic>Animals</topic><topic>Biocompatible Materials</topic><topic>Cell Tracking - methods</topic><topic>HeLa Cells</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Intravenous administration</topic><topic>Lung - cytology</topic><topic>Mesenchymal Stem Cell Transplantation - methods</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stem Cells - physiology</topic><topic>Mesenchyme</topic><topic>Microscopy, Fluorescence - methods</topic><topic>multidisciplinary</topic><topic>Nanodiamonds - administration & dosage</topic><topic>Nanodiamonds - chemistry</topic><topic>Pharmacokinetics</topic><topic>Placenta</topic><topic>Rodents</topic><topic>Science</topic><topic>Serum Albumin, Human - administration & dosage</topic><topic>Serum Albumin, Human - chemistry</topic><topic>Signal-To-Noise Ratio</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Swine</topic><topic>Swine, Miniature</topic><topic>Therapeutic applications</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Long-Jyun</creatorcontrib><creatorcontrib>Wu, Meng-Shiue</creatorcontrib><creatorcontrib>Hui, Yuen Yung</creatorcontrib><creatorcontrib>Chang, Be-Ming</creatorcontrib><creatorcontrib>Pan, Lei</creatorcontrib><creatorcontrib>Hsu, Pei-Chen</creatorcontrib><creatorcontrib>Chen, Yit-Tsong</creatorcontrib><creatorcontrib>Ho, Hong-Nerng</creatorcontrib><creatorcontrib>Huang, Yen-Hua</creatorcontrib><creatorcontrib>Ling, Thai-Yen</creatorcontrib><creatorcontrib>Hsu, Hsao-Hsun</creatorcontrib><creatorcontrib>Chang, Huan-Cheng</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Long-Jyun</au><au>Wu, Meng-Shiue</au><au>Hui, Yuen Yung</au><au>Chang, Be-Ming</au><au>Pan, Lei</au><au>Hsu, Pei-Chen</au><au>Chen, Yit-Tsong</au><au>Ho, Hong-Nerng</au><au>Huang, Yen-Hua</au><au>Ling, Thai-Yen</au><au>Hsu, Hsao-Hsun</au><au>Chang, Huan-Cheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-03-30</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>45607</spage><epage>45607</epage><pages>45607-45607</pages><artnum>45607</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Cell therapy is a promising strategy for the treatment of human diseases. While the first use of cells for therapeutic purposes can be traced to the 19th century, there has been a lack of general and reliable methods to study the biodistribution and associated pharmacokinetics of transplanted cells in various animal models for preclinical evaluation. Here, we present a new platform using albumin-conjugated fluorescent nanodiamonds (FNDs) as biocompatible and photostable labels for quantitative tracking of human placenta choriodecidual membrane-derived mesenchymal stem cells (pcMSCs) in miniature pigs by magnetic modulation. With this background-free detection technique and time-gated fluorescence imaging, we have been able to precisely determine the numbers as well as positions of the transplanted FND-labeled pcMSCs in organs and tissues of the miniature pigs after intravenous administration. The method is applicable to single-cell imaging and quantitative tracking of human stem/progenitor cells in rodents and other animal models as well.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28358111</pmid><doi>10.1038/srep45607</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/31 13/51 14/19 14/34 140/125 631/1647/245/2225 631/61/2320 639/925/350/354 639/925/352/2734 64 A549 Cells Albumin Animal models Animals Biocompatible Materials Cell Tracking - methods HeLa Cells Humanities and Social Sciences Humans Intravenous administration Lung - cytology Mesenchymal Stem Cell Transplantation - methods Mesenchymal stem cells Mesenchymal Stem Cells - physiology Mesenchyme Microscopy, Fluorescence - methods multidisciplinary Nanodiamonds - administration & dosage Nanodiamonds - chemistry Pharmacokinetics Placenta Rodents Science Serum Albumin, Human - administration & dosage Serum Albumin, Human - chemistry Signal-To-Noise Ratio Stem cell transplantation Stem cells Swine Swine, Miniature Therapeutic applications Tissue Distribution |
| title | Fluorescent nanodiamonds enable quantitative tracking of human mesenchymal stem cells in miniature pigs |
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