Investigations in the possibility of early detection of colorectal cancer by gas chromatography/triple-quadrupole mass spectrometry

In developed countries, the number of patients with colorectal cancer has been increasing, and colorectal cancer is one of the most common causes of cancer death. To improve the quality of life of colorectal cancer patients, it is necessary to establish novel screening methods that would allow early...

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Veröffentlicht in:Oncotarget 2017-03, Vol.8 (10), p.17115-17126
Hauptverfasser: Nishiumi, Shin, Kobayashi, Takashi, Kawana, Shuichi, Unno, Yumi, Sakai, Takero, Okamoto, Koji, Yamada, Yasuhide, Sudo, Kazuki, Yamaji, Taiki, Saito, Yutaka, Kanemitsu, Yukihide, Okita, Natsuko Tsuda, Saito, Hiroshi, Tsugane, Shoichiro, Azuma, Takeshi, Ojima, Noriyuki, Yoshida, Masaru
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container_end_page 17126
container_issue 10
container_start_page 17115
container_title Oncotarget
container_volume 8
creator Nishiumi, Shin
Kobayashi, Takashi
Kawana, Shuichi
Unno, Yumi
Sakai, Takero
Okamoto, Koji
Yamada, Yasuhide
Sudo, Kazuki
Yamaji, Taiki
Saito, Yutaka
Kanemitsu, Yukihide
Okita, Natsuko Tsuda
Saito, Hiroshi
Tsugane, Shoichiro
Azuma, Takeshi
Ojima, Noriyuki
Yoshida, Masaru
description In developed countries, the number of patients with colorectal cancer has been increasing, and colorectal cancer is one of the most common causes of cancer death. To improve the quality of life of colorectal cancer patients, it is necessary to establish novel screening methods that would allow early detection of colorectal cancer. We performed metabolome analysis of a plasma sample set from 282 stage 0/I/II colorectal cancer patients and 291 healthy volunteers using gas chromatography/triple-quadrupole mass spectrometry in an attempt to identify metabolite biomarkers of stage 0/I/II colorectal cancer. The colorectal cancer patients included patients with stage 0 (N=79), I (N=80), and II (N=123) in whom invasion and metastasis were absent. Our analytical system detected 64 metabolites in the plasma samples, and the levels of 29 metabolites differed significantly (Bonferroni-corrected p=0.000781) between the patients and healthy volunteers. Based on these results, a multiple logistic regression analysis of various metabolite biomarkers was carried out, and a stage 0/I/II colorectal cancer prediction model was established. The area under the curve, sensitivity, and specificity values of this model for detecting stage 0/I/II colorectal cancer were 0.996, 99.3%, and 93.8%, respectively. The model's sensitivity and specificity values for each disease stage were >90%, and surprisingly, its sensitivity for stage 0, specificity for stage 0, and sensitivity for stage II disease were all 100%. Our predictive model can aid early detection of colorectal cancer and has potential as a novel screening test for cases of colorectal cancer that do not involve lymph node or distant metastasis.
doi_str_mv 10.18632/oncotarget.15081
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subjects Adult
Aged
Aged, 80 and over
Biomarkers, Tumor - blood
Biomarkers, Tumor - metabolism
Colorectal Neoplasms - blood
Colorectal Neoplasms - diagnosis
Colorectal Neoplasms - metabolism
Early Detection of Cancer - methods
Female
Gas Chromatography-Mass Spectrometry - instrumentation
Gas Chromatography-Mass Spectrometry - methods
Humans
Logistic Models
Male
Metabolome
Metabolomics - methods
Middle Aged
Reproducibility of Results
Research Paper
ROC Curve
title Investigations in the possibility of early detection of colorectal cancer by gas chromatography/triple-quadrupole mass spectrometry
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