Elevated angiopoietin 2 in aqueous of patients with neovascular age related macular degeneration correlates with disease severity at presentation
Angiopoietin 2 (ANG2) is a proangiogenic cytokine which may have an implication in neovascular age related macular degeneration (nAMD). In 24 eyes of 24 subjects presenting with treatment naïve nAMD and 26 eyes of 26 control patients, aqueous humor samples were collected at the time of intervention...
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| Veröffentlicht in: | Scientific reports 2017-03, Vol.7 (1), p.45081-45081, Article 45081 |
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| description | Angiopoietin 2 (ANG2) is a proangiogenic cytokine which may have an implication in neovascular age related macular degeneration (nAMD). In 24 eyes of 24 subjects presenting with treatment naïve nAMD and 26 eyes of 26 control patients, aqueous humor samples were collected at the time of intervention (intravitreal injection of anti-vascular endothelial growth factor or cataract extraction). Best corrected visual acuity (BCVA) with and central macular thickness (CMT) using optical coherence tomography (OCT) were measured before each injection in the nAMD group. Aqueous cytokine levels were determined by immunoassay using a multiplex array (Quansys Biosciences, Logan, UT). Levels of ANG2 in the aqueous were significantly higher in nAMD patients than those of the control group (p |
| doi_str_mv | 10.1038/srep45081 |
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In 24 eyes of 24 subjects presenting with treatment naïve nAMD and 26 eyes of 26 control patients, aqueous humor samples were collected at the time of intervention (intravitreal injection of anti-vascular endothelial growth factor or cataract extraction). Best corrected visual acuity (BCVA) with and central macular thickness (CMT) using optical coherence tomography (OCT) were measured before each injection in the nAMD group. Aqueous cytokine levels were determined by immunoassay using a multiplex array (Quansys Biosciences, Logan, UT). Levels of ANG2 in the aqueous were significantly higher in nAMD patients than those of the control group (p < 0.0001), so were hepatocyte growth factor (HGF), interleukin-8 (IL-8) and tissue inhibitor of metalloproteinase 1 (TIMP 1), all with p < 0.001. ANG2 correlated with worse BCVA (r = 0.44, p-value = 0.027) and greater CMT (r = 0.66, p-value < 0.0001) on optical coherence tomography (OCT). ANG2 is upregulated in patients with nAMD and correlates with severity of disease at presentation.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep45081</identifier><identifier>PMID: 28345626</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/45/127 ; 692/699/3161/1626 ; Acuity ; Aged ; Aged, 80 and over ; Angiopoietin ; Angiopoietin-2 - metabolism ; Aqueous Humor - metabolism ; Biomarkers - metabolism ; Case-Control Studies ; Cataracts ; Cytokines ; Female ; Growth factors ; Hepatocyte growth factor ; Hepatocyte Growth Factor - metabolism ; Humanities and Social Sciences ; Humans ; Injection ; Interleukin 8 ; Interleukin-8 - metabolism ; Macular degeneration ; Macular Degeneration - metabolism ; Macular Degeneration - pathology ; Male ; Metalloproteinase ; Middle Aged ; multidisciplinary ; Science ; Tissue inhibitor of metalloproteinase 1 ; Tissue Inhibitor of Metalloproteinase-1 - metabolism ; Tomography ; Vascular endothelial growth factor ; Visual Acuity</subject><ispartof>Scientific reports, 2017-03, Vol.7 (1), p.45081-45081, Article 45081</ispartof><rights>The Author(s) 2017</rights><rights>Copyright Nature Publishing Group Mar 2017</rights><rights>Copyright © 2017, The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-bc5f7ef0c9d2e45061c39694cb663abc1b8bfdf212c355243f7b145e3b895c23</citedby><cites>FETCH-LOGICAL-c438t-bc5f7ef0c9d2e45061c39694cb663abc1b8bfdf212c355243f7b145e3b895c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366858/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5366858/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28345626$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ng, Danny S.</creatorcontrib><creatorcontrib>Yip, Yolanda W.</creatorcontrib><creatorcontrib>Bakthavatsalam, Malini</creatorcontrib><creatorcontrib>Chen, Li J.</creatorcontrib><creatorcontrib>Ng, Tse K.</creatorcontrib><creatorcontrib>Lai, Timothy Y.</creatorcontrib><creatorcontrib>Pang, Calvin P.</creatorcontrib><creatorcontrib>Brelén, Mårten E.</creatorcontrib><title>Elevated angiopoietin 2 in aqueous of patients with neovascular age related macular degeneration correlates with disease severity at presentation</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Angiopoietin 2 (ANG2) is a proangiogenic cytokine which may have an implication in neovascular age related macular degeneration (nAMD). In 24 eyes of 24 subjects presenting with treatment naïve nAMD and 26 eyes of 26 control patients, aqueous humor samples were collected at the time of intervention (intravitreal injection of anti-vascular endothelial growth factor or cataract extraction). Best corrected visual acuity (BCVA) with and central macular thickness (CMT) using optical coherence tomography (OCT) were measured before each injection in the nAMD group. Aqueous cytokine levels were determined by immunoassay using a multiplex array (Quansys Biosciences, Logan, UT). Levels of ANG2 in the aqueous were significantly higher in nAMD patients than those of the control group (p < 0.0001), so were hepatocyte growth factor (HGF), interleukin-8 (IL-8) and tissue inhibitor of metalloproteinase 1 (TIMP 1), all with p < 0.001. ANG2 correlated with worse BCVA (r = 0.44, p-value = 0.027) and greater CMT (r = 0.66, p-value < 0.0001) on optical coherence tomography (OCT). ANG2 is upregulated in patients with nAMD and correlates with severity of disease at presentation.</description><subject>631/45/127</subject><subject>692/699/3161/1626</subject><subject>Acuity</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Angiopoietin</subject><subject>Angiopoietin-2 - metabolism</subject><subject>Aqueous Humor - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>Case-Control Studies</subject><subject>Cataracts</subject><subject>Cytokines</subject><subject>Female</subject><subject>Growth factors</subject><subject>Hepatocyte growth factor</subject><subject>Hepatocyte Growth Factor - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Injection</subject><subject>Interleukin 8</subject><subject>Interleukin-8 - metabolism</subject><subject>Macular degeneration</subject><subject>Macular Degeneration - metabolism</subject><subject>Macular Degeneration - pathology</subject><subject>Male</subject><subject>Metalloproteinase</subject><subject>Middle Aged</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Tissue inhibitor of metalloproteinase 1</subject><subject>Tissue Inhibitor of Metalloproteinase-1 - metabolism</subject><subject>Tomography</subject><subject>Vascular endothelial growth factor</subject><subject>Visual Acuity</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkd9qFDEUxoMottRe-AIS8MYK2-b_Zm4EKa0Khd70PmQyZ6Yps8mYZFb6GL6xaWddVpuLJOT8znfOyYfQe0rOKeH6IieYhCSavkLHjAi5Ypyx1wf3I3Sa8wOpS7JG0OYtOmKaC6mYOka_r0bY2gIdtmHwcYoeig-Y4brZnzPEOePY48kWD6Fk_MuXexwgbm1282gTtgPgBOOzxMYubx0MECDVnBiwi2mJ75I7n8FmwBm2kHx5xLbgKUGu8s8J79Cb3o4ZTnfnCbq7vrq7_L66uf324_LrzcoJrsuqdbJfQ09c0zGo8yvqeKMa4VqluG0dbXXbdz2jzHEpmeD9uqVCAm91Ix3jJ-jLIjvN7QY6V8snO5op-Y1NjyZab_6NBH9vhrg1kiulpa4Cn3YCKdaPysVsfHYwjjY8_ZqhWlMhNJGyoh__Qx_inEKdztCGcK6VIutKnS2USzFXV_t9M5SYJ6vN3urKfjjsfk_-NbYCnxcg11AYIB2UfKH2B3cUtzE</recordid><startdate>20170327</startdate><enddate>20170327</enddate><creator>Ng, Danny S.</creator><creator>Yip, Yolanda W.</creator><creator>Bakthavatsalam, Malini</creator><creator>Chen, Li J.</creator><creator>Ng, Tse K.</creator><creator>Lai, Timothy Y.</creator><creator>Pang, Calvin P.</creator><creator>Brelén, Mårten E.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170327</creationdate><title>Elevated angiopoietin 2 in aqueous of patients with neovascular age related macular degeneration correlates with disease severity at presentation</title><author>Ng, Danny S. ; Yip, Yolanda W. ; Bakthavatsalam, Malini ; Chen, Li J. ; Ng, Tse K. ; Lai, Timothy Y. ; Pang, Calvin P. ; Brelén, Mårten E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-bc5f7ef0c9d2e45061c39694cb663abc1b8bfdf212c355243f7b145e3b895c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>631/45/127</topic><topic>692/699/3161/1626</topic><topic>Acuity</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Angiopoietin</topic><topic>Angiopoietin-2 - metabolism</topic><topic>Aqueous Humor - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>Case-Control Studies</topic><topic>Cataracts</topic><topic>Cytokines</topic><topic>Female</topic><topic>Growth factors</topic><topic>Hepatocyte growth factor</topic><topic>Hepatocyte Growth Factor - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Injection</topic><topic>Interleukin 8</topic><topic>Interleukin-8 - metabolism</topic><topic>Macular degeneration</topic><topic>Macular Degeneration - metabolism</topic><topic>Macular Degeneration - pathology</topic><topic>Male</topic><topic>Metalloproteinase</topic><topic>Middle Aged</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Tissue inhibitor of metalloproteinase 1</topic><topic>Tissue Inhibitor of Metalloproteinase-1 - metabolism</topic><topic>Tomography</topic><topic>Vascular endothelial growth factor</topic><topic>Visual Acuity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ng, Danny S.</creatorcontrib><creatorcontrib>Yip, Yolanda W.</creatorcontrib><creatorcontrib>Bakthavatsalam, Malini</creatorcontrib><creatorcontrib>Chen, Li J.</creatorcontrib><creatorcontrib>Ng, Tse K.</creatorcontrib><creatorcontrib>Lai, Timothy Y.</creatorcontrib><creatorcontrib>Pang, Calvin P.</creatorcontrib><creatorcontrib>Brelén, Mårten E.</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng, Danny S.</au><au>Yip, Yolanda W.</au><au>Bakthavatsalam, Malini</au><au>Chen, Li J.</au><au>Ng, Tse K.</au><au>Lai, Timothy Y.</au><au>Pang, Calvin P.</au><au>Brelén, Mårten E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elevated angiopoietin 2 in aqueous of patients with neovascular age related macular degeneration correlates with disease severity at presentation</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-03-27</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>45081</spage><epage>45081</epage><pages>45081-45081</pages><artnum>45081</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Angiopoietin 2 (ANG2) is a proangiogenic cytokine which may have an implication in neovascular age related macular degeneration (nAMD). In 24 eyes of 24 subjects presenting with treatment naïve nAMD and 26 eyes of 26 control patients, aqueous humor samples were collected at the time of intervention (intravitreal injection of anti-vascular endothelial growth factor or cataract extraction). Best corrected visual acuity (BCVA) with and central macular thickness (CMT) using optical coherence tomography (OCT) were measured before each injection in the nAMD group. Aqueous cytokine levels were determined by immunoassay using a multiplex array (Quansys Biosciences, Logan, UT). Levels of ANG2 in the aqueous were significantly higher in nAMD patients than those of the control group (p < 0.0001), so were hepatocyte growth factor (HGF), interleukin-8 (IL-8) and tissue inhibitor of metalloproteinase 1 (TIMP 1), all with p < 0.001. ANG2 correlated with worse BCVA (r = 0.44, p-value = 0.027) and greater CMT (r = 0.66, p-value < 0.0001) on optical coherence tomography (OCT). ANG2 is upregulated in patients with nAMD and correlates with severity of disease at presentation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28345626</pmid><doi>10.1038/srep45081</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/45/127 692/699/3161/1626 Acuity Aged Aged, 80 and over Angiopoietin Angiopoietin-2 - metabolism Aqueous Humor - metabolism Biomarkers - metabolism Case-Control Studies Cataracts Cytokines Female Growth factors Hepatocyte growth factor Hepatocyte Growth Factor - metabolism Humanities and Social Sciences Humans Injection Interleukin 8 Interleukin-8 - metabolism Macular degeneration Macular Degeneration - metabolism Macular Degeneration - pathology Male Metalloproteinase Middle Aged multidisciplinary Science Tissue inhibitor of metalloproteinase 1 Tissue Inhibitor of Metalloproteinase-1 - metabolism Tomography Vascular endothelial growth factor Visual Acuity |
| title | Elevated angiopoietin 2 in aqueous of patients with neovascular age related macular degeneration correlates with disease severity at presentation |
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